FastQCFastQC Report
Thu 12 Mar 2020
H3V73AFX2_n01_CD4_CFSE_low_corrected.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3V73AFX2_n01_CD4_CFSE_low_corrected.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11948325
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACGATCTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTAT138344711.578585282874378No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCAC129730310.85761393333375No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTAT119659910.014784499082507No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA10421858.722435989981859No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTATG9006577.537935233599688No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACT8911937.458727478537787No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTATG8175626.842482105232324No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGT7815716.541259967401289No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCA7501856.278578796609567No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA7241626.060782578311185No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTC6322755.291745914176254No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCC6110675.114248231446667No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTCGC1120450.0142.96474
CGCGCGG778900.0142.823274
ATACTGT1275150.0142.750781
TCGCGCG779900.0142.704773
TCGATTC810300.0142.64232
GATCTCT1484300.0142.61747
ATCTCTT2766900.0142.605098
GATCGCG780600.0142.601271
CGATTCC807850.0142.598923
CGTTACC1396850.0142.597156
GATCATG951300.0142.536122
ATCGCGC780850.0142.516572
CGATCTC1483000.0142.495576
CGCATCT1131550.0142.41558
TACACGA1484250.0142.394472
TCTCTTG2769300.0142.33599
ATACACG1484500.0142.322661
AACAATG980400.0142.286362
ACTGTAT1278900.0142.285253
CACGATC1483600.0142.282624
Produced by FastQC (version 0.11.5)