FastQCFastQC Report
Thu 12 Mar 2020
H3V73AFX2_n01_CD4_CFSE_high.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3V73AFX2_n01_CD4_CFSE_high.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8084
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACGATCTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTAT95211.77634834240475No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCAC87210.786739238000989No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTAT7919.784760019792182No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA7509.277585353785254No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTATG6107.545769421078674No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCA5787.1499257793171695No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACT5476.766452251360712No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTATG4956.123206333498269No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGT4825.962394854032657No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA4535.603661553686294No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTC3694.564571994062345No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCC3033.7481444829292427No Hit
GATAGCTCGCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA270.33399307273626916No Hit
ATACACTATCTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTAT190.23503216229589313No Hit
ATACTCTATCTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTAT100.12370113805047006No Hit
ATACAGTATCTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTAT100.12370113805047006No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTCTT202.073757E-6144.000027
AACAATG202.073757E-6144.000022
CAATGGT202.073757E-6144.000024
GTATCTC800.0144.000026
ATGGTCT202.073757E-6144.000026
TATCTCT800.0144.000027
AATGGTC202.073757E-6144.000025
TGTATCT800.0144.000025
CTGTATC800.0144.000024
ACAATGG202.073757E-6144.000023
GTAGAGT351.0913936E-11144.04
TCCTTGG750.0144.07
AAGTAGA351.0913936E-11144.02
ACACGAT600.0144.03
ATCTCTT1400.0144.08
CGATAGG550.0144.06
TCGATTC750.0144.02
CGATACA351.0913936E-11144.05
TACAGGT351.0913936E-11144.08
CGTTACC850.0144.06
Produced by FastQC (version 0.11.5)