FastQCFastQC Report
Thu 12 Mar 2020
H3V73AFX2_n01_CD4_CFSE_high_corrected.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3V73AFX2_n01_CD4_CFSE_high_corrected.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9903959
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACGATCTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTAT117434411.857318876219097No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCAC109166311.022491106839194No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTAT101373710.235674440897826No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA8626068.709708915394339No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTATG7597237.670902110963908No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACT7520957.593882406015615No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTATG6909366.976361675164447No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCA6736396.801714344738301No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGT6557376.620958346051312No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA5377065.429202604736147No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTC5219395.270003641977921No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCC3990684.029378554576003No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACTGT1075650.0143.11461
AGGTCGC932300.0143.095544
ATCTCTT2323450.0142.788968
ACACGAT1241500.0142.752153
ATACACG1240750.0142.735181
ACTGTAT1078300.0142.64673
CACGATC1240050.0142.62294
CGCGCGG578900.0142.616784
TCTCTTG2325550.0142.520699
CGTTACC1179900.0142.514946
ATCGCGC578450.0142.459822
CTGTATC1078450.0142.43994
CGATCTC1242600.0142.411356
GATCATG803850.0142.385542
CGATTCC724750.0142.382983
AACAATG815300.0142.364272
GATCTCT1244850.0142.35227
TCGATTC727350.0142.293262
ACGATCT1244050.0142.280095
GATCGCG579550.0142.256651
Produced by FastQC (version 0.11.5)