FastQCFastQC Report
Fri 13 Jul 2018
H3TN2AFXY_n02_ah9723i-0718.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3TN2AFXY_n02_ah9723i-0718.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5427676
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC8600.032.95104644
GTCGCCG9800.027.3085145
AGATCGG12250.027.0642971
CGTGTAG11050.026.87552316
AGCGTCG12550.026.35256211
GCGTCGT12450.026.20208712
TGGTCGC10700.026.0641243
GTCGTGT12150.025.92393914
GATCGGA13100.025.4577962
GTGGTCG11350.025.16639342
CGTCGTG12850.025.03722413
CTCGGTG9950.024.86581638
GTGTAGG12200.024.34239617
ATCTCGG10350.023.47235136
GTAGGGA12600.023.21186419
AGAGCGT14400.023.1257889
GAGCGTC14400.022.81159410
AAGAGCG14750.022.5795368
TGTAGGG13150.022.41211118
TCGTGTA15150.020.64179215
Produced by FastQC (version 0.11.2)