FastQCFastQC Report
Fri 13 Jul 2018
H3TN2AFXY_n02_ah9723a-0718.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3TN2AFXY_n02_ah9723a-0718.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6209181
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC4350.019.12979344
GCGTCGT7150.012.90097712
CTCGGTG6400.012.65219438
AGCGTCG7150.012.58672511
GTCGCCG6400.012.29915845
GTCGTGT8600.011.24884214
TGGTCGC7000.011.24521743
GTGGTCG7150.011.00930342
CGTGTAG7950.010.75343616
GATCGGA8700.010.6201572
CGTCGTG9700.09.97344213
ATCTCGG7900.09.68098636
GAGCGTC9950.09.49712210
GTGTAGG9050.09.19787417
TCGTGTA11500.09.19455615
GTAGGGA9300.09.19237919
AGATCGG10250.09.02117351
AGAGCGT11000.08.3865149
GAGTGTA11350.08.32399428
TGTAGGG10400.08.22017518