FastQCFastQC Report
Fri 13 Jul 2018
H3TN2AFXY_n02_ah9722i-0718.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3TN2AFXY_n02_ah9722i-0718.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7442369
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG113730.1528142450340745Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC19650.037.54216844
CGTGTAG22400.035.8541316
GCGTCGT24650.035.50214412
GTCGCCG20200.035.18364745
AGCGTCG25250.034.83742511
CTCGGTG18900.034.155738
GTGGTCG22850.033.56532342
GTCGTGT25750.033.11232814
GTGTAGG24400.032.9157117
GTAGGGA24700.032.42462519
ATCTCGG19950.032.3597736
TGTAGGG25100.032.17718
AGATCGG27800.031.9691451
GATCTCG20650.030.9360435
GGTGGTC24400.030.60410541
AAGAGCG30000.029.7780518
TCGTGTA28850.029.63191415
TGGTCGC25300.029.5144343
GATCGGA30700.028.7788352
TCGGAAG30750.028.4233534
Produced by FastQC (version 0.11.2)