FastQCFastQC Report
Fri 13 Jul 2018
H3TN2AFXY_n02_ah8643i2-0718.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3TN2AFXY_n02_ah8643i2-0718.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5567962
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG444310.797975991933853No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG13200.09.37190538
TTAGGGG10300.07.643382537
TAGGGTG6752.6291673E-86.997689738
TTGGGGG42750.06.52416238
TGGGGCG6651.0611911E-66.425583445
TTTGGGG29050.06.34925137
GTGGGCG5702.11084E-45.91830145
AGGGGGG49400.05.32714139
ATGGGGG15451.4551915E-115.240988338
GGGGAGA14607.8216544E-115.238796722
GTAGGGG11208.352799E-85.22167837
GGGTTGT18750.05.15862230
GGGGGAT16104.7293724E-115.03016421
GGGGGTA17901.8189894E-125.02704222
GGGAGTG11651.9933032E-75.020523525
Produced by FastQC (version 0.11.2)