FastQCFastQC Report
Fri 13 Jul 2018
H3TN2AFXY_n02_ah7797i-0718.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3TN2AFXY_n02_ah7797i-0718.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15485623
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA643550.41557901803498637No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC551650.3562336497537103No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG236580.15277396330777263No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGGG67100.039.39684316
CCTCGGG67650.038.84374615
TCGGGGA87100.038.64174317
GGCGGTC88100.038.3474338
TAAGCCG84650.037.1557716
CCAGGCG90600.037.04340735
GTCTCCC91400.036.9360242
CTGACCC84450.036.51443540
CAGGCGG92250.036.47802436
CGGTCTC93200.036.440840
CCCAGGC92200.036.2055134
GCGGTCT94150.036.02659639
CCAGGGA87850.035.883945
GGTCTCC96050.035.31257641
AGGCGGT95800.035.29041337
CCTGACC87950.034.9602839
TCCAGGG91150.034.622374
AGCCGAT91550.034.55176518
GACGTCA91400.034.45688225
TCCTCGG77550.034.00090414
Produced by FastQC (version 0.11.2)