FastQCFastQC Report
Fri 13 Jul 2018
H3TN2AFXY_n02_ah7797a-0718.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3TN2AFXY_n02_ah7797a-0718.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8973310
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG166010.18500419577614058No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGGG4700.011.96676316
GGCGGTC6200.011.60890238
CCTCGGG4900.011.01931415
CCAGGCG8550.09.20843135
TCGGGGA7400.08.81654517
CCCAGGC9650.08.6249834
GCGGTCT9550.08.00762339
CAGGCGG9250.07.781970536
GTCTCCC9900.07.49687342
TAAGCCG7953.092282E-117.357678416
CTGACCC7851.8553692E-107.16285740
CGGTCTC10250.07.02169640
TCCTCGG10900.06.60488614
CCTGACC7951.2943929E-86.507118739
AGGCGGT11951.8189894E-126.211698537
TCCCAGG14803.6379788E-125.471758433
GGTCTCC13704.0745363E-105.253429441
CCAGGGA11053.352343E-75.09445245