FastQCFastQC Report
Fri 13 Jul 2018
H3TN2AFXY_n01_ah9724i1-0718.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3TN2AFXY_n01_ah9724i1-0718.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7225446
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG8700.09.56861211
CGGGGGT3003.224315E-58.99966744
AGTCACG9300.08.46742928
CGTTTCG9150.07.868561733
ACGTCTG9850.07.53779315
ACACGTC10150.07.536667313
GTCACGT11000.07.36336529
CACACGT10500.07.28544512
AGCACAC11700.06.730520710
CACGTCT11050.06.71920914
TCCAGTC14000.06.58904225
CGTCTGA12500.06.47980616
AGATCGG9809.276846E-116.43118241
CAGTCAC13300.06.42833427
GTTTCGG12200.06.270260334
GATCGGA12751.8189894E-126.00243661
GAGCACA13150.05.98841339
CCAGTCA14500.05.741167526
TCGGAAG14151.8189894E-125.72547773
GAACTCC13559.094947E-125.645587421