FastQCFastQC Report
Fri 13 Jul 2018
H3TN2AFXY_n01_ah9724i-0718.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3TN2AFXY_n01_ah9724i-0718.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5852894
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG12350.022.0547811
GCACACG13000.020.76823412
ACACGTC13400.020.14828714
CACGTCT14200.019.33005715
ACGTCTG14200.019.01333416
CACACGT14800.018.24236713
GATCGGA15900.017.5518762
CAGTCAC16200.016.80474928
AGCACAC16100.016.62963511
GAGCACA16600.016.12874210
GAACTCC16750.015.98444422
CGTCTGA17150.015.87400817
TCCAGTC17450.015.343101526
CCAGTCA17600.015.34017227
AGTCACA17900.015.33445829
GTCACAT18400.014.67320730
TCGGAAG18650.014.3602954
GTCTGAA19500.014.19174718
ACTCCAG18800.014.12166224
TATCTCG13700.013.63084842