FastQCFastQC Report
Fri 13 Jul 2018
H3TN2AFXY_n01_ah9723i-0718.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3TN2AFXY_n01_ah9723i-0718.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5427676
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTAT63660.1172877673612058TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG13850.024.3767851
CACGAGT16550.020.12012332
ACACGTC17100.019.34140814
AGTCACG17600.019.17544729
GCACACG17850.018.90688112
GTCACGA17800.018.7071930
ACGTCTG18750.017.99968116
GATCGGA18950.017.8133222
CACACGT19050.017.71589913
CACGTCT19300.017.60299315
ACGAGTG19450.017.46723733
TCACGAG19550.017.26280431
CGTCTGA20000.016.87470217
CGAGTGG20400.016.32294334
TCGTATG15500.016.25747945
AGCACAC20950.015.89441511
CAGTCAC21650.015.69227528
TATCTCG19700.015.64664542
TCCAGTC22150.015.33804826
GAGCACA22200.015.20215410
Produced by FastQC (version 0.11.2)