FastQCFastQC Report
Fri 13 Jul 2018
H3TN2AFXY_n01_ah9723a-0718.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3TN2AFXY_n01_ah9723a-0718.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6209181
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG25450.033.68240411
CCGTACG25300.033.17066633
ACGTCTG27350.031.34249315
CGTACGT26750.031.20441234
ACACGTC27650.031.16517313
CACGTCT27700.030.94646814
CACACGT28400.030.57981512
CGTCTGA28900.030.20645516
GATCGGA28550.029.6437421
AGCACAC30250.028.78425410
GAACTCC30200.028.31017521
GTACGTA30150.028.20787635
CAGTCAC31450.027.25650827
ACCGTAC31400.027.15660332
CCAGTCA32450.026.76322726
GTCACCG31600.026.69992829
GAGCACA33400.026.3390249
TCCAGTC32850.026.23187425
AGAGCAC33700.025.904268
AGTCACC32850.025.88942128