FastQCFastQC Report
Fri 13 Jul 2018
H3TN2AFXY_n01_ah9722i-0718.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3TN2AFXY_n01_ah9722i-0718.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7442369
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC153300.20598279929414948TruSeq Adapter, Index 9 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC112110.15063751877930268TruSeq Adapter, Index 9 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG27050.032.6175841
GTATGCC20500.029.74301345
GCACACG37500.023.57929612
AGTCACG38300.022.9692929
GTCACGA38550.022.82032830
CGATCAG38800.022.67329234
ACACGTC39300.022.61382914
CACGTCT39350.022.58509415
ACGTCTG39450.022.5850316
CACACGT39700.022.27263513
AGCACAC40800.021.89272911
GATCTCG36350.021.66372740
TCACGAT41200.021.40712731
CCAGTCA41450.021.33229427
CACGATC41600.021.30946232
GAGCACA41750.021.23289910
TCCAGTC41650.021.17583826
CAGTCAC41900.021.1031928
GAACTCC42750.020.68373122
GTCTGAA45300.019.8670918
Produced by FastQC (version 0.11.2)