FastQCFastQC Report
Fri 13 Jul 2018
H3TN2AFXY_n01_ah9722b-0718.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3TN2AFXY_n01_ah9722b-0718.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7754210
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG27350.024.2678811
ACACGTC28450.023.56682813
ACGTCTG29350.022.6908515
GTCACAC28800.022.42108329
GATCGGA30150.022.0986021
CACACGT30450.021.87114712
CGTCTGA30600.021.83760316
CACGTCT30800.021.6226114
AGCACAC32950.020.34829310
CAGTCAC32950.019.8020327
GAACTCC33750.019.46610321
AGTCACA33300.019.4587728
TCCAGTC33850.019.27553725
ATCGGGG15300.019.26402340
CCAGTCA33950.019.21876126
GAGCACA34750.019.164799
AGATCGG17050.018.6146621
TCGGAAG37350.018.2563693
AGAGCAC36600.018.1960778
AATCGGG19750.018.11328339