FastQCFastQC Report
Fri 13 Jul 2018
H3TN2AFXY_n01_ah9041i-0718.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3TN2AFXY_n01_ah9041i-0718.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15215308
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG1904201.2515027628753883TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG256900.042.8262111
GATCGGA255600.042.508331
CACACGT261850.041.98225812
ACACGTC261400.041.96845613
ACGTCTG260600.041.87281815
AGTCACG260450.041.8623828
CACGTCT262400.041.6970514
GTCACGT262450.041.4576429
AGCACAC267500.041.33102810
GATCTCG225900.041.22342741
CGATCTC225550.041.11781740
CGTCTGA265450.041.1163816
GAGCACA268750.041.0803269
AGAGCAC271700.040.683988
CACGTGA268100.040.60912731
CAGTCAC268800.040.50337627
TCCAGTC267800.040.48659525
TCACGTG269600.040.3414630
TCGGAAG276350.040.205383
CCAGTCA270250.040.0945826
Produced by FastQC (version 0.11.2)