FastQCFastQC Report
Fri 13 Jul 2018
H3TN2AFXY_n01_ah9041a-0718.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3TN2AFXY_n01_ah9041a-0718.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17341
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG680.39213424831324606TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC206.8443414E-444.99710513
CACACGT206.8443414E-444.99710512
ACGTCTG206.8443414E-444.99710515
CACGTCT206.8443414E-444.99710514
CTCCAGT206.8443414E-444.99710524
ACTCCAG206.8443414E-444.99710523
TCCAGTC206.8443414E-444.99710525
AGCACAC206.8443414E-444.99710510
GCACACG206.8443414E-444.99710511
AGTCACG206.8443414E-444.99710528
CAGTCAC250.002051028735.9976927
CCAGTCA250.002051028735.9976926
AAGAGCA250.002051028735.997697
GAGCACA250.002051028735.997699
GTCACGT300.005011544629.99807429
GAAGAGC300.005011544629.9980746
AGAGCAC300.005011544629.9980748
ATCGGAA300.005011544629.9980742
GGAAGAG300.005011544629.9980745
Produced by FastQC (version 0.11.2)