FastQCFastQC Report
Fri 13 Jul 2018
H3TN2AFXY_n01_ah8643i2-0718.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3TN2AFXY_n01_ah8643i2-0718.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5567962
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG553970.9949241751290687TruSeq Adapter, Index 14 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCGCGTATG222610.39980517108414176TruSeq Adapter, Index 14 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCGCGGATG95720.17191209279086317TruSeq Adapter, Index 14 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTTTG68040.12219910983587892TruSeq Adapter, Index 14 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGGATG64740.11627234524948266TruSeq Adapter, Index 14 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATT60620.10887286946283038TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG218750.043.9804511
GATCGGA216850.043.853581
ACACGTC221550.043.495713
CACACGT222200.043.37859312
CACGTCT222350.043.2683814
ACGTCTG222250.043.2372315
AGCACAC223550.043.16695410
CGTCTGA223300.043.06414416
TCGGAAG226550.043.0094683
GAGCACA224800.042.907299
AGAGCAC226450.042.703958
GTTCCGT219150.042.63738335
GTCACAG224700.042.49582329
CCGTATC206950.042.4764338
AGTTCCG222000.042.46499634
AGTCACA225300.042.46254328
ATCGGAA230050.042.423972
CAGTCAC225200.042.4114627
TCCAGTC224400.042.40223725
CCAGTCA225750.042.1486726
Produced by FastQC (version 0.11.2)