FastQCFastQC Report
Fri 13 Jul 2018
H3TN2AFXY_n01_ah8643i1-0718.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3TN2AFXY_n01_ah8643i1-0718.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7818844
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC639040.8173075201398058TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGC91650.042.6172929
GCACACG94150.042.2264711
AGTCACG93750.041.97465528
GATCGGA93950.041.7839931
ACACGTC96200.041.37341313
TCACGCC94500.041.3320130
CACACGT96750.041.1149612
ACGTCTG96900.041.0513115
CACGCCA96100.040.78433231
CGTCTGA97750.040.62529416
CACGTCT98100.040.59502414
AGCACAC98800.040.3757310
GAGCACA100900.039.7360889
CAGTCAC99350.039.65398827
TCGGAAG102150.039.38983
TCCAGTC100200.039.29514725
GTATGCC84800.038.9757545
CCAGTCA101250.038.88763826
CGCCAAT101350.038.71607233
GAACTCC102000.038.64581721
Produced by FastQC (version 0.11.2)