FastQCFastQC Report
Fri 13 Jul 2018
H3TN2AFXY_n01_ah8643a2-0718.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3TN2AFXY_n01_ah8643a2-0718.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9098094
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATG671000.7375171107267082TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG97900.040.7692511
GATCGGA97100.040.615471
ACACGTC100000.040.0255913
CACGTCT101350.039.49244314
CGTCTGA101100.039.43453616
CACACGT101550.039.39250612
AGCACAC102000.039.35106310
ACGTCTG101550.039.2595715
GAGCACA105750.038.062229
CAGTCAC106150.037.51587327
GAACTCC105300.037.49841321
AGAGCAC108250.037.391038
TCGGAAG109100.037.358683
CACAGTC106000.037.3354831
TCCAGTC107150.036.9559725
GTCACAG107800.036.87903629
CCAGTCA107850.036.73697326
AGTCACA109150.036.5465828
GTCTGAA109150.036.50555817
ATCTCGT94800.036.50142342
Produced by FastQC (version 0.11.2)