FastQCFastQC Report
Fri 13 Jul 2018
H3TN2AFXY_n01_ah8038i-0718.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3TN2AFXY_n01_ah8038i-0718.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6000568
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC666571.1108448400218112TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG94050.042.55812511
ACACGTC94800.042.2217813
GATCGGA93850.042.0423241
ACGTCTG95450.041.8635415
CACACGT95600.041.79750412
CACGTCT95950.041.71573614
CGTCTGA96900.041.39997516
AGCACAC96900.041.3296310
GTATGCC90050.040.82570345
GAGCACA98450.040.7246449
ACTGACC95950.040.70708532
TCCAGTC98400.040.4252325
TCGGAAG100500.040.3293763
CGTATGC91450.040.151544
AGAGCAC100650.040.080388
CAGTCAC99900.040.0659827
CCAGTCA99650.039.9858726
AGTCACT100300.039.68187728
GTCTGAA101800.039.51775717
GAACTCC101300.039.4903721
Produced by FastQC (version 0.11.2)