FastQCFastQC Report
Fri 13 Jul 2018
H3TN2AFXY_n01_ah7797k-0718.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3TN2AFXY_n01_ah7797k-0718.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17395710
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC295920.1701109066545717TruSeq Adapter, Index 11 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGGCT48650.035.0553231
TCACGGC50400.033.70419730
GTCACGG50950.033.38452529
GCACACG52000.032.7969511
AGTCACG51800.032.79327828
ACACGTC53500.031.83535413
CACACGT54950.031.07718812
GATCGGA54700.030.6558861
CGGCTAC56000.030.29360233
ACGTCTG56800.029.82732215
CACGTCT58150.029.32831414
ACGGCTA59300.028.987232
CGTCTGA60100.028.52655816
AGCACAC60900.028.22563410
CATCTCG52500.028.02760939
GTATGCC55100.027.93015545
GAACTCC63900.026.68930221
GGCTACA62200.026.55053534
CAGTCAC65450.026.40092727
CGTATGC59500.025.94036344
Produced by FastQC (version 0.11.2)