FastQCFastQC Report
Fri 13 Jul 2018
H3TN2AFXY_n01_ah7797i-0718.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3TN2AFXY_n01_ah7797i-0718.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15485623
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATG984710.6358865897742699TruSeq Adapter, Index 16 (97% over 40bp)
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA718780.46415956271181347No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC535530.34582399429457894No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCCG142650.042.74286335
GTCCCGA138000.042.11253736
ACCCGTC148300.041.2661432
CCCGTCC149450.041.1744233
GCACACG154400.040.99111
TCACCCG149300.040.98974230
CACCCGT150100.040.80125431
ACACGTC155900.040.75535613
ACGTCTG156550.040.55739615
CCGTCCC151550.040.38118734
CACACGT157250.040.30531312
GATCGGA156000.040.2414631
CTCGGGG62850.040.20134416
CCCGATC125650.040.19943638
CACGTCT159200.039.88228614
CGTCTGA159200.039.76922616
TCCCGAT138550.039.34712237
GGCGGTC80600.039.05249438
CCTCGGG65100.038.91557715
AGCACAC163300.038.79841210