FastQCFastQC Report
Fri 13 Jul 2018
H3TN2AFXY_n01_ah6978a-0718.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3TN2AFXY_n01_ah6978a-0718.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7778934
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG23800.024.3051241
GCACACG27300.021.26291712
CACACGT28200.020.50452813
GATCGGA28450.020.0922642
ACGTCTG28700.019.99077616
ACACGTC28850.019.96456714
CACGTCT29250.019.76846915
GTATGCC17250.019.30360445
GATCTCG24000.019.21800840
CGTCTGA30250.019.1895917
CGATCTC24450.018.86430239
AGCACAC31300.018.76125711
CAGTCAC31650.018.482728
CATCACG31000.018.36219434
CACGATC25750.018.26143337
GAGCACA32750.017.93060710
TCCAGTC32550.017.62604726
CGTATGC31900.017.49148845
CCAGTCA33350.017.27069927
AGAGCAC35200.017.193949