FastQCFastQC Report
Mon 21 Nov 2022
H3TJ5BGXN_n01_R22_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3TJ5BGXN_n01_R22_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGTAGACTCTTCAGGAAAGGACGAAACACCGAACCACTACCAACTGCT34.838709677419355No Hit
TAAGTAGACTCTTCACGAAAGGACGAAACACCGAACCAATACCAACTGCT34.838709677419355No Hit
TAAGTAGACTCTTCAGCAAAGGACGAAACACCGAACCAATACCAACTGCT23.225806451612903No Hit
TAAGTAGAGTCTTCTGGAAAGGACGAAACACCGAACCACTACCAACTGCT23.225806451612903No Hit
TAATTAAACTCTTCACGAAAGGACGAAACACCGAACCAATACCAACTGGT23.225806451612903No Hit
TAAGTAAACTCTTCTCGAAAGGACGAAACACCGAACCAATACCAACTGGT11.6129032258064515No Hit
TAAGTAGACTCTTCAGGAAAGGACGAAACACCGAACCAATACCAACTGCT11.6129032258064515No Hit
TAAGTAGACTCTTCAGCAAAGGACGAAACACCGAACCAATACCAACTGGT11.6129032258064515No Hit
TAAGTAAACTCTTCACCAAAGGACGAAACACCGAACCCCTACCAACTGCT11.6129032258064515No Hit
TAAGTATACTCTTCTGCAAAGGACGAAACACCGAACCACTACCAACTGCT11.6129032258064515No Hit
TAATTATACTCTTCTCGAAAGGACGAAACACCGAACCCATACCAACTGGA11.6129032258064515No Hit
TAAGTAAACTCTTCTCCAAAGGACGAAACACCGAACCAATACCAACTGCT11.6129032258064515No Hit
TAAGTAGAGTCTTCTGGAAAGGACGAAACACCGAACCACTACCAACTGGA11.6129032258064515No Hit
TAAGTACAGTCTTCTCCAAAGGACGAAACACCTAACCAATACCAACTGCT11.6129032258064515No Hit
TAATTAAACTCTTCACCAAAGGACGAAACACCGAACCACTACCAACTGCA11.6129032258064515No Hit
TAATTAAACTCTTCAGGAAAGGACGAAACACCTAACCAATACCAACTGGT11.6129032258064515No Hit
TAAGTAGAGTCTTCTGGAAAGGACGAAACACCGAACCACTACCAACTGGT11.6129032258064515No Hit
TAAGTAGACTCTTATCCAAAGGACGAAACACCGAACCACTACCAACTGCT11.6129032258064515No Hit
TAAGTACACTCTTCACGAAAGGACGAAACACCGAACCAATACCAACTGCT11.6129032258064515No Hit
TAAGTAAACTCTTCTCCAAAGGACGAAACACCTAACCAATACCAACTGCA11.6129032258064515No Hit
TAAGTAGACTCTTCACCAAAGGACGAAACACCGAACCAATACCAACTGCT11.6129032258064515No Hit
TAAGTACACTCTTGACCAAAGGACGAAACACCTAACCAATACCAACTGCT11.6129032258064515No Hit
GATAGCTCCGTTCTAGAGGAAAGGACGAAACAACGAAAACCTACCAACTC11.6129032258064515No Hit
TAAGTAGAGTCTTCAGGAAAGGACGAAACACCGAACCAATACCAACTGCT11.6129032258064515No Hit
TAATTATACTCTTCACGAAAGGACGAAACACAGAACCAATACCAACTGCT11.6129032258064515No Hit
TAAGTAGACTCTTCTCCAAAGGACGAAACACCGAACCACTACCAACTGCT11.6129032258064515No Hit
TAATTATACTCTTCTGCAAAGGACGAAACACCTAACCAATACCAACTGCT11.6129032258064515No Hit
TAAGTAGACTCTTCTCCAAAGGACAAACACCGAAACCCTAACCAATGGTA11.6129032258064515No Hit
TAAGTAGAGTCTTGTCCAAAGGACGAAACACCTAACCACTACCAACTGGA11.6129032258064515No Hit
TAAGTAGAGTCTTCTCGAAAGGACGAAACACCGAACCACTACCAACTGGT11.6129032258064515No Hit
TAAGTAGACTCTTGTGGAAAGGACGAAACACCGAACCACTACCAACTGCT11.6129032258064515No Hit
TAAGTACACTCTTCACGAAAGGACGAAACACCGAACCACTACCAACTGGT11.6129032258064515No Hit
TAAGTAGAGTCTTCTCGAAAGGACGAAACACCGAACCACTACCAACTGCT11.6129032258064515No Hit
TAAGTAGACTCTTCAAGAAAGGACGAAACACCGAACCACTACCAACTGCT11.6129032258064515No Hit
TAATTAAAGTCTTCACGAAAGGACGAAACACCGAACCACTACCAACTGCT11.6129032258064515No Hit
TAAGTACACTCTTCACCAAAGGACGAAACACCGAACCACTACCAACTGGT11.6129032258064515No Hit
TAATTAGAGTCTTCTCGAAAGGACGAAACACCGAACCACTACCAACTGCT11.6129032258064515No Hit
TAAGTACACTCTTCTCGAAAGGACGAAACACCGAACCACTACCAACTGGT11.6129032258064515No Hit
TAATTAAACTCTTCACGAAAGGACGAAACACCGAACCAATACCAACTGCT11.6129032258064515No Hit
TAATTACAGTCTTCAGGAAAGGACGAAACACCTAACCACTACCAACTGCT11.6129032258064515No Hit
TAAGTAGACTCTTCTCGAAAGGACGAAACACCGAACCAATACCAACTGCT11.6129032258064515No Hit
TAAGTAGACTCTTCACGAAAGGACGAAACACCGAACCACTACCAACTGCT11.6129032258064515No Hit
TAAGTAGACTCTTCACGAAAGGACGAAACACCGAACCACTACCAACAGCT11.6129032258064515No Hit
TAAGTATACTCTTCACGAAAGGACGAAACACCGAACCAATACCAACTGGT11.6129032258064515No Hit
TAAGTAGACTCTTCAGCAAAGGACGAAACACCGAACCACTACCAACTGCT11.6129032258064515No Hit
TAAGTAGAGTCTTCACCAAAGGACGAAACACCGAACCAATACCAACTGCT11.6129032258064515No Hit
TAAGTAGAGTCTTCTGGAAAGGACGAAACACCTAACCACTACCAACTGCT11.6129032258064515No Hit
TAAGTAGAGTCTTCACGAAAGGACGAAACACCGAACCAATACCAACTGCT11.6129032258064515No Hit
TAAGTAGACTCTTGAGGAAAGGACGAAACACCGAACCCATACCAACTGCT11.6129032258064515No Hit
TAATTAGAGTCTTCACGAAAGGACGAAACACCGAACCACTACCAACTGCA11.6129032258064515No Hit
TAAGTATAGTCTTCTCGAAAGGACGAAACACCGAACCAATACCAACTGCT11.6129032258064515No Hit
TAAGTAAACTCTTCACGAAAGGACGAAACACCGAACCAATACCAACTGCT11.6129032258064515No Hit
TAATTAAACTCTTCACCAAAGGACGAAACACCGAACCAATACCAACTGGA11.6129032258064515No Hit
TAAGTACACTCTTGTGGAAAGGACGAAACACCGAACCACTACCAACTGGT11.6129032258064515No Hit
TAAGTACACTCTTCTCGAAAGGACGAAACACCGAACCACTACCAACTGCT11.6129032258064515No Hit

[WARN]Adapter Content

Adapter graph