FastQCFastQC Report
Mon 21 Nov 2022
H3TJ5BGXN_n01_R21_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3TJ5BGXN_n01_R21_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14330402
Sequences flagged as poor quality0
Sequence length151
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATAGCTCCGTTCTACAGGAAAGGACGAAACACCGAACACCTACCAACTC3807072.656638662334804No Hit
GATAGCTCCGTTCTACAGGAAAGGACGAAACACCGAAAACCTACCAACTC2900142.0237673723319136No Hit
GATAGCTCCGTTCTACAGGAAAGGACGAAACAACGAAAACCTACCAACTC2292431.5996969240639585No Hit
GATAGCTCCGTTCTAGAGGAAAGGACGAAACACCGAACACCTACCAACTC2139381.4928960122681834No Hit
GATAGCTCCGTTCTACAGGAAAGGACGAAACAACGAACACCTACCAACTC2079491.4511037443332016No Hit
GATAGCTCCGTTCTTCAGGAAAGGACGAAACACCGAACACCTACCAACTC1746881.2190027886168162No Hit
GATAGCTCCGTTCTAGAGGAAAGGACGAAACACCGAAAACCTACCAACTC1543621.0771644787075758No Hit
GATAGCTCCGTTCTTCAGGAAAGGACGAAACACCGAAAACCTACCAACTC1274000.8890190240301703No Hit
GATAGCTCCGTTCTAGAGGAAAGGACGAAACAACGAAAACCTACCAACTC1163020.8115752789070398No Hit
GATAGCTCCGTTCTACTGGAAAGGACGAAACACCGAACACCTACCAACTC1148960.8017639700547131No Hit
GATAGCTCCGTTCTTGAGGAAAGGACGAAACACCGAACACCTACCAACTC1125640.7854908745756052No Hit
GATAGCTCCGTTCTAGAGGAAAGGACGAAACAACGAACACCTACCAACTC1112650.7764262300527229No Hit
GATAGCTCCGTTCTTCAGGAAAGGACGAAACAACGAAAACCTACCAACTC954290.665919909294938No Hit
GATAGCTCCGTTCTTCAGGAAAGGACGAAACAACGAACACCTACCAACTC908770.6341552735226827No Hit
GATAGCTCCGTTCTACTGGAAAGGACGAAACACCGAAAACCTACCAACTC868420.605998352314192No Hit
GATAGCTCCGTTCTTCTGGAAAGGACGAAACACCGAACACCTACCAACTC856030.5973523980695028No Hit
GATAGCTCCGTTCTTGAGGAAAGGACGAAACACCGAAAACCTACCAACTC768760.5364538971063059No Hit
GATAGCTCCGTTCTACAGGAAAGGACGAAACACCGAACACCTACCAACAC725760.5064477605024619No Hit
GATAGCTCCGTTCTAGTGGAAAGGACGAAACACCGAACACCTACCAACTC700720.48897441955920007No Hit
GATAGCTCGGTTCTACAGGAAAGGACGAAACACCGAACACCTACCAACTC682810.4764765147551339No Hit
GATAGCACCGTTCTACAGGAAAGGACGAAACACCGAACACCTACCAACAC652350.45522100496552714No Hit
GATAGCACCGTTCTACAGGAAAGGACGAAACACCGAACACCTACCAACTC633530.4420880865728679No Hit
GATAGCTCCGTTCTACTGGAAAGGACGAAACAACGAAAACCTACCAACTC623230.43490057013055183No Hit
GATAGCTCCGTTCTTCTGGAAAGGACGAAACACCGAAAACCTACCAACTC615780.42970183250965327No Hit
GATAGCTCCGTTCTTGTGGAAAGGACGAAACACCGAACACCTACCAACTC607130.4236657143323683No Hit
GATAGCTCCGTTCTACTGGAAAGGACGAAACAACGAACACCTACCAACTC572780.39969569590580917No Hit
GATAGCTCCGTTCTACAGGAAAGGACGAAACACCGAAAACCTACCAACAC563840.3934572107607309No Hit
GATAGCTCCGTTCTTGAGGAAAGGACGAAACAACGAAAACCTACCAACTC541420.3778121506989127No Hit
GATAGCTCCGTTCTTGAGGAAAGGACGAAACAACGAACACCTACCAACTC540390.3770933990546811No Hit
GATAGCTCCGTTCTACAGGAAAGGACGAAACACCGAACCCCTACCAACTC538730.37593502261834666No Hit
GATAGCACCGTTCTACAGGAAAGGACGAAACACCGAAAACCTACCAACAC515560.35976659970878694No Hit
GATAGCTCGGTTCTACAGGAAAGGACGAAACACCGAAAACCTACCAACTC504730.3522092401874002No Hit
GATAGCTCGGTTCTAGAGGAAAGGACGAAACACCGAACACCTACCAACTC503240.35116949266322045No Hit
GATAGCTCCGTTCTAGTGGAAAGGACGAAACACCGAAAACCTACCAACTC491770.34316553017842766No Hit
GATAGCACCGTTCTACAGGAAAGGACGAAACAACGAAAACCTACCAACAC456100.3182743931398435No Hit
GATAGCACCGTTCTACAGGAAAGGACGAAACAACGAACACCTACCAACAC449840.3139060579040281No Hit
GATAGCTCCGTTCTACAGGAAAGGACGAAACAACGAACACCTACCAACAC447270.31211266787910064No Hit
GATAGCTCCGTTCTACAGGAAAGGACGAAACAACGAAAACCTACCAACAC444880.3104448849376312No Hit
GATAGCTCGGTTCTACAGGAAAGGACGAAACACCGAACACCTACCAACAC439960.3070116246564472No Hit
GATAGCTCCGTTCTTCTGGAAAGGACGAAACAACGAAAACCTACCAACTC421790.29433228739849726No Hit
GATAGCGCGGTTCTACAGGAAAGGACGAAACACCGAACACCTACCAACTC419000.292385377604899No Hit
GATAGCGCCGTTCTACAGGAAAGGACGAAACACCGAACACCTACCAACTC415400.28987323593574No Hit
GATAGCTCCGTTCTACAGGAAAGGACGAAACACCGAAACCCTACCAACTC410420.28639810662673665No Hit
GATAGCTCCGTTCTTGTGGAAAGGACGAAACACCGAAAACCTACCAACTC406910.28394876849930656No Hit
GATAGCTCCGTTCTTCTGGAAAGGACGAAACAACGAACACCTACCAACTC400540.2795036733791557No Hit
GATAGCTCGGTTCTACAGGAAAGGACGAAACAACGAAAACCTACCAACTC400010.2791338303000851No Hit
GATAGCACCGTTCAACAGGAAAGGACGAAACACCGAACACCTACCAACAC395350.27588200247278477No Hit
GATAGCTCGGTTCTTCAGGAAAGGACGAAACACCGAACACCTACCAACTC392520.2739071799939736No Hit
GATAGCACCGTTCAACAGGAAAGGACGAAACAACGAAAACCTACCAACAC388310.2709693698753182No Hit
GATAGCACCGTTCTACAGGAAAGGACGAAACAACGAACACCTACCAACTC384710.2684572282061592No Hit
GATAGCACCGTTCAACAGGAAAGGACGAAACACCGAAAACCTACCAACAC369090.2575573246305302No Hit
GATAGCACCGTTCTACAGGAAAGGACGAAACACCGAAAACCTACCAACTC367890.2567199440741439No Hit
GATAGCGCGGTTCTAGAGGAAAGGACGAAACACCGAACACCTACCAACTC367160.25621053756900886No Hit
GATAGCTCGGTTCTACAGGAAAGGACGAAACACCGAAAACCTACCAACAC353310.24654577031404978No Hit
GATAGCTCCGTTCTAGAGGAAAGGACGAAACACCGAACACCTACCAACAC350280.24443138440917428No Hit
GATAGCTCGGTTCTTGAGGAAAGGACGAAACACCGAACACCTACCAACTC344690.24053058665067456No Hit
GATAGCTCCGTTCTAGTGGAAAGGACGAAACAACGAAAACCTACCAACTC337690.23564586673842086No Hit
GATAGCTCGGTTCTACAGGAAAGGACGAAACAACGAACACCTACCAACTC337020.23517832926110518No Hit
GATAGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACACCTACCAACTC334630.2335105463196357No Hit
GATAGCTCGGTTCTAGAGGAAAGGACGAAACACCGAAAACCTACCAACTC327390.22845835029610476No Hit
GATAGCTCCGTTCTAGAGGAAAGGACGAAACACCGAACCCCTACCAACTC324520.22645561513208073No Hit
GATAGCACCGTTCTACAGGAAAGGACGAAACAACGAAAACCTACCAACTC323200.22553449652005572No Hit
GATAGCTCCGTTCTAGTGGAAAGGACGAAACAACGAACACCTACCAACTC319540.2229804858230774No Hit
GATAGCTCGGTTCTTGTGGAAAGGACGAAACACCGAACACCTACCAACTC317280.22140341910854977No Hit
GATAGCTCCGTTCTACAGGAAAGGACGAAACAACGAAACCCTACCAACTC316720.22101264151556949No Hit
GATAGCTCGGTTCTAGAGGAAAGGACGAAACACCGAACACCTACCAACAC308360.21517889030607792No Hit
GATAGCACCGTTCAACAGGAAAGGACGAAACAACGAACACCTACCAACAC305620.21326687136899578No Hit
GATAGCACGGTTCTACAGGAAAGGACGAAACACCGAACACCTACCAACAC300870.20995223999996648No Hit
GATAGCGCGGTTCTTGAGGAAAGGACGAAACACCGAACACCTACCAACTC298710.2084449549984711No Hit
GATAGCTCGGTTCTACTGGAAAGGACGAAACACCGAACACCTACCAACTC298450.20826352254458735No Hit
GATAGCTCGGTTCTTCTGGAAAGGACGAAACACCGAACACCTACCAACTC292090.20382540559573972No Hit
GATAGCTCCGTTCTACAGGAAAGGACGAAACAACGAACCCCTACCAACTC287700.20076198839362636No Hit
GATAGCACCGTTCAACAGGAAAGGACGAAACACCGAACACCTACCAACTC276310.1928138512792593No Hit
GATAGCTCCGTTCTTCAGGAAAGGACGAAACACCGAACCCCTACCAACTC271020.18912239865985617No Hit
GATAGCGCGGTTCTTCAGGAAAGGACGAAACACCGAACACCTACCAACTC269710.1882082582191344No Hit
GATAGCGCCGTTCTACAGGAAAGGACGAAACACCGAAAACCTACCAACTC268080.18707081629670963No Hit
GATAGCTCGGTTCTTCAGGAAAGGACGAAACACCGAAAACCTACCAACTC267270.18650558442114884No Hit
GATAGCTCCGTTCTAGAGGAAAGGACGAAACACCGAAAACCTACCAACAC265740.18543792421175623No Hit
GATAGCTCCGTTCTTCAGGAAAGGACGAAACACCGAACACCTACCAACAC263960.18419580971978314No Hit
GATAGCGCCGTTCTAGAGGAAAGGACGAAACACCGAACACCTACCAACTC263920.1841678970345703No Hit
GATAGCTCGGTTCTACAGGAAAGGACGAAACAACGAAAACCTACCAACAC263440.18383294481201573No Hit
GATAGCGCGGTTCTACAGGAAAGGACGAAACACCGAAAACCTACCAACTC262200.18296765157041653No Hit
GATAGCTCCGTTCTTGTGGAAAGGACGAAACAACGAAAACCTACCAACTC261910.18276528460262315No Hit
GATAGCACGGTTCTACAGGAAAGGACGAAACACCGAACACCTACCAACTC260710.18192790404623682No Hit
GATAGCTCGGTTCTAGTGGAAAGGACGAAACACCGAACACCTACCAACTC260340.18166971170801768No Hit
GATAGCTCCGTTCTTGTGGAAAGGACGAAACAACGAACACCTACCAACTC257800.17989725619699992No Hit
GATAGCACGGTTCTACAGGAAAGGACGAAACACCGAAAACCTACCAACAC251660.1756126590168231No Hit
GATAGCACCGTTCTAGAGGAAAGGACGAAACACCGAACACCTACCAACTC251430.17545216107684908No Hit
GATAGCTCGGTTCTACAGGAAAGGACGAAACAACGAACACCTACCAACAC250460.17477527846043678No Hit
GATAGCACCGTTCTAGAGGAAAGGACGAAACACCGAACACCTACCAACAC246460.17198400993914895No Hit
GATAGCTCCGTTCAACAGGAAAGGACGAAACACCGAACACCTACCAACTC245610.17139086537837528No Hit
GATAGCTCCGTTCAACAGGAAAGGACGAAACACCGAACACCTACCAACAC244170.1703860087107117No Hit
GATAGCGCCGTTCTACAGGAAAGGACGAAACACCGAACACCTACCAACAC243710.17006501283076358No Hit
GATAGCTCGGTTCTAGAGGAAAGGACGAAACACCGAAAACCTACCAACAC240880.16809019035195244No Hit
GATAGCGCGGTTCTACAGGAAAGGACGAAACACCGAACACCTACCAACAC240180.16760171836072707No Hit
GATAGCGCGGTTCTTCTGGAAAGGACGAAACACCGAACACCTACCAACTC236880.16529892183066464No Hit
GATAGCTCGGTTCTAGAGGAAAGGACGAAACAACGAAAACCTACCAACTC234520.1636520734031048No Hit
GATAGCTCGGTTCTTCAGGAAAGGACGAAACACCGAACACCTACCAACAC232920.16253556599458968No Hit
GATAGCTCCGTTCAACAGGAAAGGACGAAACACCGAAAACCTACCAACAC231160.16130740784522304No Hit
GATAGCTCCGTTCTAGAGGAAAGGACGAAACACCGAAACCCTACCAACTC228560.15949308330638598No Hit
GATAGCGCGGTTCTAGTGGAAAGGACGAAACACCGAACACCTACCAACTC228100.15917208742643785No Hit
GATAGCTCGGTTCTAGAGGAAAGGACGAAACAACGAACACCTACCAACTC225180.15713446140589776No Hit
GATAGCTCCGTTCTACTGGAAAGGACGAAACACCGAACCCCTACCAACTC214010.1493398440602015No Hit
GATAGCGCGGTTCTACTGGAAAGGACGAAACACCGAACACCTACCAACTC211930.14788838442913185No Hit
GATAGCTCCGTTCAACAGGAAAGGACGAAACAACGAAAACCTACCAACAC208500.14549487167212757No Hit
GATAGCGCGGTTCTAGAGGAAAGGACGAAACACCGAACACCTACCAACAC208210.14529250470433416No Hit
GATAGCGCCGTTCTACAGGAAAGGACGAAACACCGAAAACCTACCAACAC208150.14525063567651486No Hit
GATAGCTCGGTTCTTGAGGAAAGGACGAAACACCGAAAACCTACCAACTC207770.14498546516699254No Hit
GATAGCACGGTTCTACAGGAAAGGACGAAACAACGAAAACCTACCAACAC207100.1445179276896768No Hit
GATAGCGCCGTTCTACAGGAAAGGACGAAACAACGAACACCTACCAACTC206040.14377824153153554No Hit
GATAGCTCCGTTCTAGAGGAAAGGACGAAACAACGAACACCTACCAACAC205770.1435898309063486No Hit
GATAGCTCGGTTCTACTGGAAAGGACGAAACACCGAAAACCTACCAACTC205260.14323394416988441No Hit
GATAGCGCGGTTCTAGAGGAAAGGACGAAACACCGAAAACCTACCAACTC200470.13989140011564227No Hit
GATAGCGCCGTTCTACAGGAAAGGACGAAACAACGAAAACCTACCAACTC200230.13972392400436498No Hit
GATAGCTCCGTTCTAGAGGAAAGGACGAAACAACGAAAACCTACCAACAC198900.13879582722103678No Hit
GATAGCTCCGTTCTTCAGGAAAGGACGAAACACCGAAAACCTACCAACAC197040.13749788735863794No Hit
GATAGCTCGGTTCTTCAGGAAAGGACGAAACAACGAAAACCTACCAACTC196820.1373443675899671No Hit
GATAGCACGGTTCTACAGGAAAGGACGAAACAACGAACACCTACCAACAC196500.1371210661082641No Hit
GATAGCACCGTTCTTCAGGAAAGGACGAAACACCGAACACCTACCAACTC196200.1369117209691675No Hit
GATAGCGCCGTTCTTCAGGAAAGGACGAAACACCGAACACCTACCAACTC194090.1354393268241882No Hit
GATAGCTCCGTTCTTGAGGAAAGGACGAAACACCGAACCCCTACCAACTC193700.13516717814336263No Hit
GATAGCTCGGTTCTTGAGGAAAGGACGAAACACCGAACACCTACCAACAC193120.1347624442077759No Hit
GATAGCTCCGTTCTTCAGGAAAGGACGAAACACCGAAACCCTACCAACTC192580.13438562295740203No Hit
GATAGCACCGTTCTAGAGGAAAGGACGAAACACCGAAAACCTACCAACAC192070.13402973622093783No Hit
GATAGCGCGGTTCTACAGGAAAGGACGAAACACCGAAAACCTACCAACAC191130.1333737881184352No Hit
GATAGCGCGGTTCTACAGGAAAGGACGAAACAACGAAAACCTACCAACTC186810.13035921811544435No Hit
GATAGCTCGGTTCTTCAGGAAAGGACGAAACACCGAAAACCTACCAACAC182520.1273655826263632No Hit
GATAGCTCGGTTCTTCTGGAAAGGACGAAACACCGAAAACCTACCAACTC182420.12729580091333098No Hit
GATAGCACCGTTCTTCAGGAAAGGACGAAACACCGAACACCTACCAACAC180710.12610253362048043No Hit
GATAGCTCCGTTCTACTGGAAAGGACGAAACACCGAACACCTACCAACAC179020.12492322267023634No Hit
GATAGCACCGTTCAACAGGAAAGGACGAAACAACGAACACCTACCAACTC178810.12477668107286871No Hit
GATAGCTCGGTTCTTCAGGAAAGGACGAAACAACGAACACCTACCAACTC177940.12416958016948862No Hit
GATAGCGCGGTTCTACAGGAAAGGACGAAACAACGAACACCTACCAACTC173530.1210922066247688No Hit
GATAGCTCGGTTCTTGTGGAAAGGACGAAACACCGAAAACCTACCAACTC173520.12108522845346557No Hit
GATAGCCCCGTTCTACAGGAAAGGACGAAACACCGAACACCTACCAACAC173030.12074329805960782No Hit
GATAGCTCCGTTCAACAGGAAAGGACGAAACAACGAACACCTACCAACAC167530.11690530384283707No Hit
GATAGCTCCGTTCTTCTGGAAAGGACGAAACACCGAACCCCTACCAACTC167040.11656337344897931No Hit
GATAGCACGGTTCTAGAGGAAAGGACGAAACACCGAACACCTACCAACAC166720.11634007196727628No Hit
GATAGCTCCGTTCTAGAGGAAAGGACGAAACAACGAAACCCTACCAACTC166190.11597022888820564No Hit
GATAGCTCCGTTCTAGAGGAAAGGACGAAACAACGAACCCCTACCAACTC165450.1154538442117674No Hit
GATAGCTCGGTTCTAGAGGAAAGGACGAAACAACGAACACCTACCAACAC163710.1142396424050072No Hit
GATAGCACCGTTCTAGAGGAAAGGACGAAACAACGAAAACCTACCAACAC163130.11383490846942046No Hit
GATAGCTCGGTTCTAGAGGAAAGGACGAAACAACGAAAACCTACCAACAC163070.11379303944160114No Hit
GATAGCACCGTTCTAGAGGAAAGGACGAAACAACGAACACCTACCAACAC162490.11338830550601442No Hit
GATAGCGCGGTTCTAGAGGAAAGGACGAAACACCGAAAACCTACCAACAC162180.11317198219561461No Hit
GATAGCTCCGTTCTACTGGAAAGGACGAAACACCGAAACCCTACCAACTC160360.11190195501842866No Hit
GATAGCGCGGTTCTTGAGGAAAGGACGAAACACCGAACACCTACCAACAC159040.11098083640640366No Hit
GATAGCTCCGTTCTTCAGGAAAGGACGAAACAACGAACACCTACCAACAC158210.11040164818823645No Hit
GATAGCGCCGTTCTAGAGGAAAGGACGAAACACCGAAAACCTACCAACTC157840.11014345585001734No Hit
GATAGCTCGGTTCTAGTGGAAAGGACGAAACACCGAAAACCTACCAACTC157530.10992713253961753No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACACCTACCAACTC156070.10890831952934747No Hit
GATAGCTCGGTTCTACTGGAAAGGACGAAACACCGAACACCTACCAACAC155920.10880364695979917No Hit
GATAGCGCGGTTCTTCAGGAAAGGACGAAACACCGAAAACCTACCAACTC153160.10687767168011057No Hit
GATAGCGCGGTTCTTCAGGAAAGGACGAAACACCGAACACCTACCAACAC152790.10661947934189145No Hit
GATAGCGCGGTTCTTGTGGAAAGGACGAAACACCGAAAACCTACCAACTC152420.10636128700367233No Hit
GATAGCTCCGTTCTTGAGGAAAGGACGAAACACCGAACACCTACCAACAC149440.10428179195531291No Hit
GATAGCGCGGTTCTTGAGGAAAGGACGAAACACCGAAAACCTACCAACTC148510.1036328220241135No Hit
GATAGCGCCGTTCTACAGGAAAGGACGAAACAACGAAAACCTACCAACAC148130.10336765151459115No Hit
GATAGCACGGTTCTAGAGGAAAGGACGAAACACCGAACACCTACCAACTC148100.1033467170006815No Hit
GATAGCTCCGTTCTTCAGGAAAGGACGAAACAACGAAAACCTACCAACAC147460.10290011403727543No Hit
GATAGCTCCGTTCTAGTGGAAAGGACGAAACACCGAACCCCTACCAACTC146050.10191619188352147No Hit
GATAGCACCGTTCTAGAGGAAAGGACGAAACAACGAACACCTACCAACTC145960.1018533883417925No Hit
GATAGCTCGGTTCTTGAGGAAAGGACGAAACACCGAAAACCTACCAACAC145810.10174871577224422No Hit
GATAGCGCCGTTCTTGAGGAAAGGACGAAACACCGAACACCTACCAACTC145180.10130909098014138No Hit
GATAGCTCGGTTCTACTGGAAAGGACGAAACAACGAAAACCTACCAACTC143860.1003879723681164No Hit
GATAGCCCCGTTCTACAGGAAAGGACGAAACACCGAAAACCTACCAACAC143640.10023445259944558No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)