FastQCFastQC Report
Mon 21 Nov 2022
H3TJ5BGXN_n01_R20_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3TJ5BGXN_n01_R20_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14476247
Sequences flagged as poor quality0
Sequence length151
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATAGCTCCGTTCTACAGGAAAGGACGAAACACCGAACACCTACCAACTC4004162.7660207787280777No Hit
GATAGCTCCGTTCTACAGGAAAGGACGAAACACCGAAAACCTACCAACTC3189632.2033542257188623No Hit
GATAGCTCCGTTCTACAGGAAAGGACGAAACAACGAAAACCTACCAACTC2741201.893584711562327No Hit
GATAGCTCCGTTCTACAGGAAAGGACGAAACAACGAACACCTACCAACTC2422531.6734516895159361No Hit
GATAGCTCCGTTCTAGAGGAAAGGACGAAACACCGAACACCTACCAACTC1939031.339456283109842No Hit
GATAGCTCCGTTCTTCAGGAAAGGACGAAACACCGAACACCTACCAACTC1661891.148011635888777No Hit
GATAGCTCCGTTCTAGAGGAAAGGACGAAACACCGAAAACCTACCAACTC1448601.0006737243430566No Hit
GATAGCTCCGTTCTTCAGGAAAGGACGAAACACCGAAAACCTACCAACTC1260360.870640021547021No Hit
GATAGCTCCGTTCTAGAGGAAAGGACGAAACAACGAAAACCTACCAACTC1201480.8299664961505561No Hit
GATAGCTCCGTTCTAGAGGAAAGGACGAAACAACGAACACCTACCAACTC1119090.7730525736401154No Hit
GATAGCTCCGTTCTACTGGAAAGGACGAAACACCGAACACCTACCAACTC1065480.7360194945554603No Hit
GATAGCTCCGTTCTTCAGGAAAGGACGAAACAACGAAAACCTACCAACTC1035960.7156274689151132No Hit
GATAGCTCCGTTCTTCAGGAAAGGACGAAACAACGAACACCTACCAACTC960690.6636319482528862No Hit
GATAGCTCCGTTCTTGAGGAAAGGACGAAACACCGAACACCTACCAACTC922000.6369054078726344No Hit
GATAGCACCGTTCTACAGGAAAGGACGAAACACCGAACACCTACCAACTC916230.6329195681726072No Hit
GATAGCACCGTTCTACAGGAAAGGACGAAACACCGAACACCTACCAACAC877370.6060755940403615No Hit
GATAGCTCCGTTCTACTGGAAAGGACGAAACACCGAAAACCTACCAACTC842370.5818980568651531No Hit
GATAGCTCCGTTCTACAGGAAAGGACGAAACACCGAACACCTACCAACAC751340.5190157366063178No Hit
GATAGCTCCGTTCTTCTGGAAAGGACGAAACACCGAACACCTACCAACTC718150.496088523496456No Hit
GATAGCACCGTTCTACAGGAAAGGACGAAACACCGAAAACCTACCAACAC706310.48790960806347117No Hit
GATAGCACCGTTCTACAGGAAAGGACGAAACAACGAAAACCTACCAACAC668580.4618462229885964No Hit
GATAGCTCCGTTCTACTGGAAAGGACGAAACAACGAAAACCTACCAACTC665470.4596978761138851No Hit
GATAGCTCCGTTCTTGAGGAAAGGACGAAACACCGAAAACCTACCAACTC655570.4528590870271832No Hit
GATAGCACCGTTCTACAGGAAAGGACGAAACAACGAACACCTACCAACAC650890.4496262049134696No Hit
GATAGCACCGTTCAACAGGAAAGGACGAAACAACGAAAACCTACCAACAC614730.42464735507759716No Hit
GATAGCTCCGTTCTACAGGAAAGGACGAAACACCGAACCCCTACCAACTC602740.4163648216281472No Hit
GATAGCTCCGTTCTACAGGAAAGGACGAAACACCGAAAACCTACCAACAC600260.4146516704225895No Hit
GATAGCACCGTTCAACAGGAAAGGACGAAACACCGAACACCTACCAACAC597640.41284180906833107No Hit
GATAGCTCGGTTCTACAGGAAAGGACGAAACACCGAACACCTACCAACTC594600.4107418172679701No Hit
GATAGCTCCGTTCTACTGGAAAGGACGAAACAACGAACACCTACCAACTC592480.4092773493019289No Hit
GATAGCACCGTTCTACAGGAAAGGACGAAACAACGAACACCTACCAACTC591010.4082618927405701No Hit
GATAGCACCGTTCTACAGGAAAGGACGAAACACCGAAAACCTACCAACTC579810.4005250808445034No Hit
GATAGCACCGTTCAACAGGAAAGGACGAAACACCGAAAACCTACCAACAC566410.3912685380402807No Hit
GATAGCTCCGTTCTAGTGGAAAGGACGAAACACCGAACACCTACCAACTC558070.3855073763248168No Hit
GATAGCTCCGTTCTTCTGGAAAGGACGAAACACCGAAAACCTACCAACTC542450.3747172868769094No Hit
GATAGCACCGTTCTACAGGAAAGGACGAAACAACGAAAACCTACCAACTC531270.36699429071637146No Hit
GATAGCTCCGTTCTACAGGAAAGGACGAAACAACGAAAACCTACCAACAC521510.36025221177837047No Hit
GATAGCTCCGTTCTACAGGAAAGGACGAAACAACGAACACCTACCAACAC520680.3596788587539298No Hit
GATAGCTCCGTTCTTGAGGAAAGGACGAAACAACGAAAACCTACCAACTC507500.35057428904052274No Hit
GATAGCTCCGTTCTTGAGGAAAGGACGAAACAACGAACACCTACCAACTC500500.34573878160548105No Hit
GATAGCACCGTTCAACAGGAAAGGACGAAACAACGAACACCTACCAACAC484900.33496250789310245No Hit
GATAGCTCCGTTCTACAGGAAAGGACGAAACACCGAAACCCTACCAACTC481960.33293159477038486No Hit
GATAGCTCGGTTCTACAGGAAAGGACGAAACACCGAAAACCTACCAACTC454120.3137000909144476No Hit
GATAGCTCCGTTCTTGTGGAAAGGACGAAACACCGAACACCTACCAACTC438430.30286164639218993No Hit
GATAGCACCGTTCAACAGGAAAGGACGAAACACCGAACACCTACCAACTC413560.28568177926226324No Hit
GATAGCTCCGTTCTAGTGGAAAGGACGAAACACCGAAAACCTACCAACTC407880.2817581103721151No Hit
GATAGCTCCGTTCTTCTGGAAAGGACGAAACAACGAAAACCTACCAACTC404080.2791331206216639No Hit
GATAGCTCCGTTCTACAGGAAAGGACGAAACAACGAAACCCTACCAACTC397420.2745324806906099No Hit
GATAGCTCGGTTCTACAGGAAAGGACGAAACAACGAAAACCTACCAACTC395830.27343412971607906No Hit
GATAGCGCCGTTCTACAGGAAAGGACGAAACACCGAACACCTACCAACTC390600.26982131487532646No Hit
GATAGCTCGGTTCTACAGGAAAGGACGAAACACCGAACACCTACCAACAC386270.26683020813336494No Hit
GATAGCTCGGTTCTAGAGGAAAGGACGAAACACCGAACACCTACCAACTC381410.26347298439989314No Hit
GATAGCTCCGTTCTTCTGGAAAGGACGAAACAACGAACACCTACCAACTC372220.2571246539244598No Hit
GATAGCGCGGTTCTACAGGAAAGGACGAAACACCGAACACCTACCAACTC357040.24663851065818373No Hit
GATAGCACGGTTCTACAGGAAAGGACGAAACACCGAACACCTACCAACAC354930.24518095055990688No Hit
GATAGCTCCGTTCTACAGGAAAGGACGAAACAACGAACCCCTACCAACTC350920.2424108955864044No Hit
GATAGCTCGGTTCTACAGGAAAGGACGAAACAACGAACACCTACCAACTC329450.22757970349635512No Hit
GATAGCACCGTTCTAGAGGAAAGGACGAAACACCGAACACCTACCAACTC322110.22250932855732564No Hit
GATAGCTCCGTTCTAGAGGAAAGGACGAAACACCGAACACCTACCAACAC321120.22182544964865547No Hit
GATAGCTCGGTTCTACAGGAAAGGACGAAACACCGAAAACCTACCAACAC309050.21348765325709074No Hit
GATAGCTCCGTTCTAGTGGAAAGGACGAAACAACGAAAACCTACCAACTC308640.2132044306787526No Hit
GATAGCTCCGTTCTAGAGGAAAGGACGAAACACCGAACCCCTACCAACTC308610.21318370707545956No Hit
GATAGCTCCGTTCTTGTGGAAAGGACGAAACACCGAAAACCTACCAACTC307650.2125205517700824No Hit
GATAGCTCGGTTCTTCAGGAAAGGACGAAACACCGAACACCTACCAACTC305300.21089720284546126No Hit
GATAGCACGGTTCTACAGGAAAGGACGAAACACCGAACACCTACCAACTC304520.2103583891598423No Hit
GATAGCTCCGTTCAACAGGAAAGGACGAAACACCGAACACCTACCAACAC302730.2091218808300245No Hit
GATAGCTCCGTTCAACAGGAAAGGACGAAACACCGAACACCTACCAACTC300720.20773339940939112No Hit
GATAGCACGGTTCTACAGGAAAGGACGAAACACCGAAAACCTACCAACAC298020.2058682751130179No Hit
GATAGCACCGTTCTAGAGGAAAGGACGAAACACCGAACACCTACCAACAC292570.20210348718144971No Hit
GATAGCTCCGTTCTAGTGGAAAGGACGAAACAACGAACACCTACCAACTC286490.19790350358072778No Hit
GATAGCTCCGTTCAACAGGAAAGGACGAAACACCGAAAACCTACCAACAC282730.19530614530133397No Hit
GATAGCACCGTTCAACAGGAAAGGACGAAACAACGAACACCTACCAACTC280570.1938140458642354No Hit
GATAGCTCCGTTCAACAGGAAAGGACGAAACAACGAAAACCTACCAACAC276430.19095418860979643No Hit
GATAGCTCCGTTCTTCAGGAAAGGACGAAACACCGAACCCCTACCAACTC271050.1872377557525787No Hit
GATAGCACGGTTCTACAGGAAAGGACGAAACAACGAAAACCTACCAACAC270940.1871617692071709No Hit
GATAGCGCCGTTCTACAGGAAAGGACGAAACACCGAAAACCTACCAACTC260880.18021245423623955No Hit
GATAGCGCGGTTCTAGAGGAAAGGACGAAACACCGAACACCTACCAACTC260340.1798394293769649No Hit
GATAGCTCGGTTCTACAGGAAAGGACGAAACAACGAAAACCTACCAACAC259840.17949403598874764No Hit
GATAGCACCGTTCTTCAGGAAAGGACGAAACACCGAACACCTACCAACTC259160.17902430098077216No Hit
GATAGCTCCGTTCTTCAGGAAAGGACGAAACACCGAACACCTACCAACAC252230.17423714862008088No Hit
GATAGCACGGTTCTACAGGAAAGGACGAAACAACGAACACCTACCAACAC251380.17364997986011155No Hit
GATAGCTCGGTTCTAGAGGAAAGGACGAAACACCGAAAACCTACCAACTC249660.17246182660464415No Hit
GATAGCTCCGTTCTAGAGGAAAGGACGAAACACCGAAAACCTACCAACAC247990.17130821268799848No Hit
GATAGCTCGGTTCTACAGGAAAGGACGAAACAACGAACACCTACCAACAC245910.16987137619301468No Hit
GATAGCTCGGTTCTAGAGGAAAGGACGAAACACCGAACACCTACCAACAC235900.16295660056090505No Hit
GATAGCTCGGTTCTTGAGGAAAGGACGAAACACCGAACACCTACCAACTC234520.1620033148094254No Hit
GATAGCTCCGTTCTAGAGGAAAGGACGAAACACCGAAACCCTACCAACTC231200.15970990271166277No Hit
GATAGCACCGTTCTAGAGGAAAGGACGAAACACCGAAAACCTACCAACAC230510.15923325983592296No Hit
GATAGCTCGGTTCTACTGGAAAGGACGAAACACCGAACACCTACCAACTC228840.15807964591927728No Hit
GATAGCGCGGTTCTACAGGAAAGGACGAAACACCGAAAACCTACCAACTC228400.1577756997376461No Hit
GATAGCACCGTTCTTCAGGAAAGGACGAAACACCGAACACCTACCAACAC224710.15522669653260268No Hit
GATAGCGCCGTTCTACAGGAAAGGACGAAACAACGAAAACCTACCAACTC223860.15463952777263335No Hit
GATAGCTCCGTTCAACAGGAAAGGACGAAACAACGAACACCTACCAACAC223730.15454972549169685No Hit
GATAGCGCCGTTCTACAGGAAAGGACGAAACACCGAACACCTACCAACAC219790.1518280255925448No Hit
GATAGCTCCGTTCTTGTGGAAAGGACGAAACAACGAAAACCTACCAACTC218560.15097835785753033No Hit
GATAGCGCCGTTCTACAGGAAAGGACGAAACAACGAACACCTACCAACTC217170.15001816423828634No Hit
GATAGCTCGGTTCTTCAGGAAAGGACGAAACACCGAAAACCTACCAACTC215960.14918231223880057No Hit
GATAGCACCGTTCTAGAGGAAAGGACGAAACAACGAAAACCTACCAACAC213690.1476142262562942No Hit
GATAGCTCCGTTCTACTGGAAAGGACGAAACACCGAACCCCTACCAACTC212680.1469165316120953No Hit
GATAGCGCCGTTCTAGAGGAAAGGACGAAACACCGAACACCTACCAACTC211820.14632245498436164No Hit
GATAGCACCGTTCTAGAGGAAAGGACGAAACAACGAACACCTACCAACAC211630.14619120549683906No Hit
GATAGCTCCGTTCTAGAGGAAAGGACGAAACAACGAACACCTACCAACAC209960.14503759158019341No Hit
GATAGCTCCGTTCTTGTGGAAAGGACGAAACAACGAACACCTACCAACTC209190.14450568576233883No Hit
GATAGCTCCGTTCTAGAGGAAAGGACGAAACAACGAAAACCTACCAACAC208110.14375963604378952No Hit
GATAGCTCCGTTCTTCAGGAAAGGACGAAACACCGAAACCCTACCAACTC205910.14223990513563356No Hit
GATAGCACCGTTCTAGAGGAAAGGACGAAACAACGAACACCTACCAACTC202640.13998103237669268No Hit
GATAGCACCGTTCAACAGGAAAGGACGAAACACCGAAAACCTACCAACTC201220.13900011515415564No Hit
GATAGCCCCGTTCTACAGGAAAGGACGAAACACCGAACACCTACCAACAC201060.13888958926992612No Hit
GATAGCTCGGTTCTAGAGGAAAGGACGAAACAACGAAAACCTACCAACTC200820.13872380044358182No Hit
GATAGCGCGGTTCTTCAGGAAAGGACGAAACACCGAACACCTACCAACTC199610.13788794844409605No Hit
GATAGCTCGGTTCTTCTGGAAAGGACGAAACACCGAACACCTACCAACTC198430.1370728200479033No Hit
GATAGCACCGTTCTAGAGGAAAGGACGAAACACCGAAAACCTACCAACTC197310.13629913885829664No Hit
GATAGCTCGGTTCTTGTGGAAAGGACGAAACACCGAACACCTACCAACTC193950.13397809528947663No Hit
GATAGCTCGGTTCTAGAGGAAAGGACGAAACAACGAACACCTACCAACTC193020.13333566358739252No Hit
GATAGCTCCGTTCTTCAGGAAAGGACGAAACACCGAAAACCTACCAACAC192690.1331077039511691No Hit
GATAGCGCGGTTCTTGAGGAAAGGACGAAACACCGAACACCTACCAACTC188380.13013041294473632No Hit
GATAGCTCCGTTCAACAGGAAAGGACGAAACAACGAACACCTACCAACTC187810.1297366644821686No Hit
GATAGCTCGGTTCTTCAGGAAAGGACGAAACACCGAACACCTACCAACAC186210.12863140563987338No Hit
GATAGCACCGTTCAACAGGAAAGGACGAAACAACGAAAACCTACCAACTC186180.12861068203658035No Hit
GATAGCGCGGTTCTACAGGAAAGGACGAAACAACGAAAACCTACCAACTC186010.12849324828458647No Hit
GATAGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACACCTACCAACTC185990.12847943254905778No Hit
GATAGCTCGGTTCTAGAGGAAAGGACGAAACACCGAAAACCTACCAACAC185490.1281340391608405No Hit
GATAGCGCCGTTCTACAGGAAAGGACGAAACACCGAAAACCTACCAACAC185290.12799588180555363No Hit
GATAGCACCGTTCTACTGGAAAGGACGAAACACCGAACACCTACCAACTC185250.12796825033449624No Hit
GATAGCGCGGTTCTACAGGAAAGGACGAAACACCGAACACCTACCAACAC182630.12615838898023776No Hit
GATAGCACGGTTCTACAGGAAAGGACGAAACAACGAACACCTACCAACTC180140.12443832990691578No Hit
GATAGCTCCGTTCTAGAGGAAAGGACGAAACAACGAAACCCTACCAACTC179830.1242241860062211No Hit
GATAGCACCGTTCTAGAGGAAAGGACGAAACAACGAAAACCTACCAACTC178260.12313965076721889No Hit
GATAGCACGGTTCTACAGGAAAGGACGAAACACCGAAAACCTACCAACTC176950.12223472009008965No Hit
GATAGCTCCGTTCAACAGGAAAGGACGAAACACCGAAAACCTACCAACTC176050.12161301199129858No Hit
GATAGCACCGTTCTTCAGGAAAGGACGAAACACCGAAAACCTACCAACAC174550.12057683182664677No Hit
GATAGCTCGGTTCTTCAGGAAAGGACGAAACAACGAAAACCTACCAACTC174140.12029360924830862No Hit
GATAGCTCCGTTCTAGAGGAAAGGACGAAACAACGAACCCCTACCAACTC173800.12005874174432088No Hit
GATAGCTCGGTTCTAGTGGAAAGGACGAAACACCGAACACCTACCAACTC171730.11862881311710141No Hit
GATAGCACGGTTCTAGAGGAAAGGACGAAACACCGAACACCTACCAACAC171610.11854591870392928No Hit
GATAGCTCCGTTCTACTGGAAAGGACGAAACACCGAAACCCTACCAACTC169600.11715743728329586No Hit
GATAGCCCCGTTCTACAGGAAAGGACGAAACACCGAAAACCTACCAACAC167980.11603836270547194No Hit
GATAGCGCCGTTCTTCAGGAAAGGACGAAACACCGAACACCTACCAACTC167890.11597619189559283No Hit
GATAGCTCCGTTCTTCAGGAAAGGACGAAACAACGAACACCTACCAACAC167160.11547191754879563No Hit
GATAGCTCCGTTCTACTGGAAAGGACGAAACACCGAACACCTACCAACAC166870.11527158938362961No Hit
GATAGCGCGGTTCTACAGGAAAGGACGAAACAACGAACACCTACCAACTC166200.11480876224341849No Hit
GATAGCTCCGTTCTTGAGGAAAGGACGAAACACCGAACCCCTACCAACTC165840.11456007900390204No Hit
GATAGCTCGGTTCTACTGGAAAGGACGAAACACCGAAAACCTACCAACTC164110.11336501788067031No Hit
GATAGCACCGTTCTTCAGGAAAGGACGAAACACCGAAAACCTACCAACTC163890.11321304478985471No Hit
GATAGCTCCGTTCTTCAGGAAAGGACGAAACAACGAAACCCTACCAACTC162120.1119903521955656No Hit
GATAGCACCGTTCTTCAGGAAAGGACGAAACAACGAAAACCTACCAACAC161320.11143772277441798No Hit
GATAGCTCCGTTCTTCAGGAAAGGACGAAACAACGAAAACCTACCAACAC161220.11136864409677454No Hit
GATAGCACCGTTCTTCAGGAAAGGACGAAACAACGAACACCTACCAACTC160670.11098871136973555No Hit
GATAGCACGGTTCAACAGGAAAGGACGAAACACCGAACACCTACCAACAC159040.10986272892414727No Hit
GATAGCTCGGTTCTTCAGGAAAGGACGAAACAACGAACACCTACCAACTC158250.109317007370764No Hit
GATAGCACCGTTCTTCAGGAAAGGACGAAACAACGAACACCTACCAACAC157520.10881273302396678No Hit
GATAGCGCGGTTCTACTGGAAAGGACGAAACACCGAACACCTACCAACTC157440.10875747008185202No Hit
GATAGCACGGTTCAACAGGAAAGGACGAAACACCGAAAACCTACCAACAC156040.10779036859484367No Hit
GATAGCCCCGTTCTACAGGAAAGGACGAAACAACGAAAACCTACCAACAC155260.10725155490922474No Hit
GATAGCGCGGTTCTTCTGGAAAGGACGAAACACCGAACACCTACCAACTC154820.10694760872759355No Hit
GATAGCACGGTTCAACAGGAAAGGACGAAACAACGAAAACCTACCAACAC153750.10620846687680861No Hit
GATAGCACGGTTCTAGAGGAAAGGACGAAACACCGAACACCTACCAACTC153020.1057041925300114No Hit
GATAGCGCCGTTCTACAGGAAAGGACGAAACAACGAAAACCTACCAACAC152650.10544860142273063No Hit
GATAGCTCCGTTCTTCTGGAAAGGACGAAACACCGAACCCCTACCAACTC152630.10543478568720195No Hit
GATAGCACCGTTCTACTGGAAAGGACGAAACACCGAACACCTACCAACAC152460.10531735193520808No Hit
GATAGCTCCGTTCTTCAGGAAAGGACGAAACAACGAACCCCTACCAACTC151910.10493741920816908No Hit
GATAGCGCGGTTCTAGAGGAAAGGACGAAACACCGAAAACCTACCAACTC150580.10401867279551116No Hit
GATAGCTCGGTTCTTCAGGAAAGGACGAAACACCGAAAACCTACCAACAC146980.10153184040034685No Hit
GATAGCGCGGTTCTACAGGAAAGGACGAAACACCGAAAACCTACCAACAC146770.10138677517729561No Hit
GATAGCCCCGTTCTACAGGAAAGGACGAAACAACGAACACCTACCAACAC145990.10084796149167667No Hit
GATAGCGCCGTTCTACAGGAAAGGACGAAACAACGAACACCTACCAACAC145310.1003782264837012No Hit
GATAGCCCCGTTCTACAGGAAAGGACGAAACACCGAACACCTACCAACTC145090.1002262533928856No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)