FastQCFastQC Report
Mon 21 Nov 2022
H3TJ5BGXN_n01_R18_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3TJ5BGXN_n01_R18_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10972397
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACGATCTCTACACGAAAGGACGAAACACAGAACAACTACCAACTCC6712906.117988621811624No Hit
ATAAACAAACTCTACACGAAAGGACGAAACACAGAACAACTACCAACTCC3504213.193659507580705No Hit
ATACACGATCTCTACTCGAAAGGACGAAACACAGAACAACTACCAACTCC2949312.687935917739761No Hit
ATACACGATCTCTAGACGAAAGGACGAAACACAGAACAACTACCAACTCC2569552.3418310511367753No Hit
ATACACGATCTCTACAGGAAAGGACGAAACACAGAACAACTACCAACTCC2003431.825881801396723No Hit
ATACACTATCTCTACACGAAAGGACGAAACACAGAACAACTACCAACTCC1592221.451114100228054No Hit
ATAAACTAACTCTACACGAAAGGACGAAACACAGAACAACTACCAACTCC1540771.4042237079099489No Hit
ATACACAAACTCTACACGAAAGGACGAAACACAGAACAACTACCAACTCC1414131.2888068122216139No Hit
ATACACGATCTCTAGTCGAAAGGACGAAACACAGAACAACTACCAACTCC1314351.1978695265947814No Hit
ATACACGATCTCTAGAGGAAAGGACGAAACACAGAACAACTACCAACTCC1280601.1671105228875696No Hit
ATAAACGATCTCTACACGAAAGGACGAAACACAGAACAACTACCAACTCC1264711.1526287282532706No Hit
ATACACGAACTCTACACGAAAGGACGAAACACAGAACAACTACCAACTCC1250141.1393499524306312No Hit
ATAAACCAACTCTACACGAAAGGACGAAACACAGAACAACTACCAACTCC1095920.9987972546017064No Hit
ATACACGATCTCTACACGAAAGGACGAAACACAGAACACCTACCAACTCC1060910.966889914756092No Hit
ATAAACTATCTCTACACGAAAGGACGAAACACAGAACAACTACCAACTCC1042560.9501661305182451No Hit
ATACACTAACTCTACACGAAAGGACGAAACACAGAACAACTACCAACTCC1033090.9415353819224733No Hit
ATACACGATCTCTACTGGAAAGGACGAAACACAGAACAACTACCAACTCC995980.9077141485128546No Hit
ATACACCAACTCTACACGAAAGGACGAAACACAGAACAACTACCAACTCC966880.8811930519830807No Hit
ATAAACGAACTCTACACGAAAGGACGAAACACAGAACAACTACCAACTCC894560.8152822031503235No Hit
ATAAACAAACTCTACTCGAAAGGACGAAACACAGAACAACTACCAACTCC842950.768245990370199No Hit
ATAAACAAACTCTAGACGAAAGGACGAAACACAGAACAACTACCAACTCC791750.7215834425239991No Hit
ATACACGATCTCTAGTGGAAAGGACGAAACACAGAACAACTACCAACTCC771270.7029184233855191No Hit
ATAAACAATCTCTACACGAAAGGACGAAACACAGAACAACTACCAACTCC707380.6446904901454076No Hit
ATAAACAAACTCTACAGGAAAGGACGAAACACAGAACAACTACCAACTCC606020.5523132274561338No Hit
ATAAACAAACTCTACACGAAAGGACGAAACACAGAACACCTACCAACTCC603750.5502443996512338No Hit
ATACACTATCTCTACTCGAAAGGACGAAACACAGAACAACTACCAACTCC568630.5182368082379812No Hit
ATACACGATCTCTACACGAAAGGACGAAACACAGAACCACTACCAACTCC563560.5136161223477422No Hit
ATAAACGATCTCTACTCGAAAGGACGAAACACAGAACAACTACCAACTCC540940.4930007545297531No Hit
ATACACTATCTCTAGACGAAAGGACGAAACACAGAACAACTACCAACTCC520110.4740167531306058No Hit
ATACACGATCTCTACTCGAAAGGACGAAACACAGAACACCTACCAACTCC519090.47308714768523236No Hit
ATACACGAACTCTACTCGAAAGGACGAAACACAGAACAACTACCAACTCC497030.4529821514843111No Hit
ATAAACGATCTCTAGACGAAAGGACGAAACACAGAACAACTACCAACTCC473330.4313824955476912No Hit
ATAAACTAACTCTACTCGAAAGGACGAAACACAGAACAACTACCAACTCC469440.42783723556484515No Hit
ATAAACAAACTCTACACGAAAGGACGAAACACAGAACAACTACCAACTGC465310.42407324488897No Hit
ATACACGAACTCTAGACGAAAGGACGAAACACAGAACAACTACCAACTCC457030.4165270359794674No Hit
ATACACGATCTCTAGACGAAAGGACGAAACACAGAACACCTACCAACTCC446370.4068117476974265No Hit
ATACACTATCTCTACAGGAAAGGACGAAACACAGAACAACTACCAACTCC437440.39867314316097024No Hit
ATAAACTAACTCTAGACGAAAGGACGAAACACAGAACAACTACCAACTCC437240.398490867583446No Hit
ATAAACGATCTCTACAGGAAAGGACGAAACACAGAACAACTACCAACTCC396560.36141601511502No Hit
ATACACGATCTCTACAGGAAAGGACGAAACACAGAACACCTACCAACTCC388060.35366930307024075No Hit
ATAAACTATCTCTACTCGAAAGGACGAAACACAGAACAACTACCAACTCC382000.3481463530712569No Hit
ATACACAAACTCTACTCGAAAGGACGAAACACAGAACAACTACCAACTCC370810.3379480345087769No Hit
ATACACAAACTCTAGACGAAAGGACGAAACACAGAACAACTACCAACTCC358040.32630973888385556No Hit
ATAAACTATCTCTAGACGAAAGGACGAAACACAGAACAACTACCAACTCC349410.3184445477136855No Hit
ATACACTAACTCTACTCGAAAGGACGAAACACAGAACAACTACCAACTCC342360.31201933360595685No Hit
ATACACGAACTCTACAGGAAAGGACGAAACACAGAACAACTACCAACTCC342050.31173680646079427No Hit
ATAAACGAACTCTACTCGAAAGGACGAAACACAGAACAACTACCAACTCC332000.3025774586902023No Hit
ATAAACTAACTCTACAGGAAAGGACGAAACACAGAACAACTACCAACTCC328120.2990413124862325No Hit
ATAAACCAACTCTACTCGAAAGGACGAAACACAGAACAACTACCAACTCC324660.2958879449950635No Hit
ATACACTAACTCTAGACGAAAGGACGAAACACAGAACAACTACCAACTCC323160.29452087816363187No Hit
ATACACAATCTCTACACGAAAGGACGAAACACAGAACAACTACCAACTCC321960.2934272246984866No Hit
ATACACCAACTCTACTCGAAAGGACGAAACACAGAACAACTACCAACTCC302500.27569181100538015No Hit
ATAAACGAACTCTAGACGAAAGGACGAAACACAGAACAACTACCAACTCC300420.27379614499912824No Hit
ATACACCATCTCTACACGAAAGGACGAAACACAGAACAACTACCAACTCC297450.27108935267289364No Hit
ATAAACCAACTCTAGACGAAAGGACGAAACACAGAACAACTACCAACTCC294020.2679633265183533No Hit
ATAAACTATCTCTACAGGAAAGGACGAAACACAGAACAACTACCAACTCC290220.26450009054539314No Hit
ATACACCAACTCTAGACGAAAGGACGAAACACAGAACAACTACCAACTCC284550.2593325779225815No Hit
ATACACGATCTCTAGAGGAAAGGACGAAACACAGAACACCTACCAACTCC275220.2508294222310768No Hit
ATAAACCATCTCTACACGAAAGGACGAAACACAGAACAACTACCAACTCC272140.2480223783372038No Hit
CTAAACAAACTCTACACGAAAGGACGAAACACAGAACAACTACCAACTCC266710.24307359640742127No Hit
ATACACAAACTCTACAGGAAAGGACGAAACACAGAACAACTACCAACTCC266490.24287309327214465No Hit
ATACACGATCTCTAGTCGAAAGGACGAAACACAGAACACCTACCAACTCC265790.24223512875080985No Hit
ATACACAAACTCTACACGAAAGGACGAAACACAGAACACCTACCAACTCC265020.24153336777734163No Hit
ATACACGATCTCTACTCGAAAGGACGAAACACAGAACCACTACCAACTCC261640.23845291051718234No Hit
ATAAACTAACTCTACACGAAAGGACGAAACACAGAACACCTACCAACTCC252440.23006823395106832No Hit
ATAAACGATCTCTAGAGGAAAGGACGAAACACAGAACAACTACCAACTCC249510.2273978967403385No Hit
ATACACTATCTCTAGAGGAAAGGACGAAACACAGAACAACTACCAACTCC246790.22491894888600913No Hit
ATACACGAACTCTACACGAAAGGACGAAACACAGAACACCTACCAACTCC246030.2242263016914171No Hit
ATACACTAACTCTACAGGAAAGGACGAAACACAGAACAACTACCAACTCC244360.22270430061908986No Hit
ATAAACGATCTCTAGTCGAAAGGACGAAACACAGAACAACTACCAACTCC242570.22107293420024812No Hit
ATAAACAAACTCTACACGAAAGGACGAAACACAGAACCACTACCAACTCC242070.22061724525643758No Hit
ATACACTATCTCTACACGAAAGGACGAAACACAGAACACCTACCAACTCC239390.2181747525176131No Hit
ATAAACAATCTCTACTCGAAAGGACGAAACACAGAACAACTACCAACTCC232640.21202295177617073No Hit
ATACACAAACTCTACACGAAAGGACGAAACACAGAACAACTACCAACTGC232180.211603717947865No Hit
ATAAACGAACTCTACAGGAAAGGACGAAACACAGAACAACTACCAACTCC229240.20892426695825897No Hit
ATACACGATCTCTAGACGAAAGGACGAAACACAGAACCACTACCAACTCC226040.2060078577178715No Hit
ATAAACAATCTCTAGACGAAAGGACGAAACACAGAACAACTACCAACTCC224680.20476838379070683No Hit
ATACACGAACTCTAGTCGAAAGGACGAAACACAGAACAACTACCAACTCC222060.20238057372513954No Hit
ATACACTATCTCTAGTCGAAAGGACGAAACACAGAACAACTACCAACTCC220930.20135071671212773No Hit
ATACACGATCTCTACTGGAAAGGACGAAACACAGAACACCTACCAACTCC219150.1997284640721622No Hit
ATAAACAAACTCTAGAGGAAAGGACGAAACACAGAACAACTACCAACTCC218230.19888999641555077No Hit
ATAAACCAACTCTACAGGAAAGGACGAAACACAGAACAACTACCAACTCC216750.19754115714187157No Hit
ATAAACAAACTCTAGTCGAAAGGACGAAACACAGAACAACTACCAACTCC216680.1974773606897381No Hit
ATAAACTAACTCTACACGAAAGGACGAAACACAGAACAACTACCAACTGC212570.1937315975716154No Hit
ATACACGATCTCTACACGAAAGGACGAAACACCGAACAACTACCAACTCC211230.19251035120220314No Hit
ATACACCAACTCTACAGGAAAGGACGAAACACAGAACAACTACCAACTCC205620.1873975212526488No Hit
ATACACGAACTCTAGAGGAAAGGACGAAACACAGAACAACTACCAACTCC202800.1848274356095573No Hit
ATAAACGATCTCTACACGAAAGGACGAAACACAGAACACCTACCAACTCC200940.1831322727385821No Hit
ATACACGATCTCTAGTGGAAAGGACGAAACACAGAACACCTACCAACTCC196950.17949587496697395No Hit
ATAAACGATCTCTACTGGAAAGGACGAAACACAGAACAACTACCAACTCC193820.17664326217871992No Hit
ATACACTAACTCTACACGAAAGGACGAAACACAGAACACCTACCAACTCC193090.17597795632075655No Hit
ATACACGAACTCTACACGAAAGGACGAAACACAGAACAACTACCAACTGC190850.1739364698524853No Hit
ATACACCAACTCTACACGAAAGGACGAAACACAGAACACCTACCAACTCC190650.17375419427496105No Hit
ATAAACCAACTCTACACGAAAGGACGAAACACAGAACACCTACCAACTCC190610.17371773915945624No Hit
ATAAACAAACTCTTCACGAAAGGACGAAACACAGAACAACTACCAACTCC189480.17268788214644437No Hit
ATAAACAAACTCTACACGAAAGGACGAAACACAGAACAACTACCAACTCG185660.16920641861573182No Hit
ATACACTATCTCTACTGGAAAGGACGAAACACAGAACAACTACCAACTCC180140.1641756126760634No Hit
ATACACGATCTCTACAGGAAAGGACGAAACACAGAACCACTACCAACTCC175120.1596004956802055No Hit
ATAAACAATCTCTACAGGAAAGGACGAAACACAGAACAACTACCAACTCC168620.15367653941066842No Hit
ATAAACTATCTCTAGAGGAAAGGACGAAACACAGAACAACTACCAACTCC166880.15209074188620775No Hit
ATAAACAAACTCTACTGGAAAGGACGAAACACAGAACAACTACCAACTCC159680.14552882109533585No Hit
ATACACTAACTCTACACGAAAGGACGAAACACAGAACAACTACCAACTGC158300.14427111961041875No Hit
ATAAACCAACTCTACACGAAAGGACGAAACACAGAACAACTACCAACTGC157980.14397947868638No Hit
ATACACGAACTCTACTGGAAAGGACGAAACACAGAACAACTACCAACTCC157960.1439612511286276No Hit
ATAAACTATCTCTACACGAAAGGACGAAACACAGAACACCTACCAACTCC157480.14352378974256946No Hit
ATAAACGAACTCTACACGAAAGGACGAAACACAGAACACCTACCAACTCC157380.14343265195380736No Hit
ATACACCAACTCTACACGAAAGGACGAAACACAGAACAACTACCAACTGC157170.14324126259740694No Hit
ATAAACTATCTCTAGTCGAAAGGACGAAACACAGAACAACTACCAACTCC154990.14125445880239293No Hit
ATAAACGATCTCTAGTGGAAAGGACGAAACACAGAACAACTACCAACTCC153930.1402883982415146No Hit
ATAAACTAACTCTAGTCGAAAGGACGAAACACAGAACAACTACCAACTCC153240.13965954749905604No Hit
ATAAACAAACTCTACTCGAAAGGACGAAACACAGAACACCTACCAACTCC150780.13741755789550816No Hit
ATAAACTAACTCTAGAGGAAAGGACGAAACACAGAACAACTACCAACTCC148750.13556746078363732No Hit
ATACACGATCTCTACACGAAAGGACGAAACACAGAACAACTACCAACTGC143190.13050019972846408No Hit
ATAAACAAACTCTAGACGAAAGGACGAAACACAGAACACCTACCAACTCC140250.12782074873885807No Hit
ATAAACGAACTCTAGTCGAAAGGACGAAACACAGAACAACTACCAACTCC132540.12079402522529946No Hit
ATACACTATCTCTACACGAAAGGACGAAACACAGAACCACTACCAACTCC131470.11981885088554489No Hit
ATAAACAAACTCTACTCGAAAGGACGAAACACAGAACAACTACCAACTGC130420.11886190410354273No Hit
ATACACGAACTCTTCACGAAAGGACGAAACACAGAACAACTACCAACTCC129930.1184153289386084No Hit
ATACACGATCTCTAGTCGAAAGGACGAAACACAGAACCACTACCAACTCC129480.11800520888917891No Hit
ATACACTATCTCTAGTGGAAAGGACGAAACACAGAACAACTACCAACTCC126050.11487918273463858No Hit
ATAAACAATCTCTACACGAAAGGACGAAACACAGAACACCTACCAACTCC125530.1144052662330756No Hit
ATACACTAACTCTAGTCGAAAGGACGAAACACAGAACAACTACCAACTCC125310.11420476309779896No Hit
ATAAACGAACTCTACACGAAAGGACGAAACACAGAACAACTACCAACTGC125170.114077170193532No Hit
ATACACGATCTCTAGAGGAAAGGACGAAACACAGAACCACTACCAACTCC123350.11241846243806163No Hit
ATACACTAACTCTAGAGGAAAGGACGAAACACAGAACAACTACCAACTCC122240.11140683298280221No Hit
ATACACCATCTCTACTCGAAAGGACGAAACACAGAACAACTACCAACTCC121470.11070507200933397No Hit
ATAAACGAACTCTAGAGGAAAGGACGAAACACAGAACAACTACCAACTCC121290.11054102398956216No Hit
ATAAACTATCTCTACTGGAAAGGACGAAACACAGAACAACTACCAACTCC120770.11006710748799921No Hit
ATAAACAAACTCTAGACGAAAGGACGAAACACAGAACAACTACCAACTGC116520.10619375146560957No Hit
ATAAACAAACTCTACAGGAAAGGACGAAACACAGAACACCTACCAACTCC115250.1050363015483308No Hit
ATACACCATCTCTAGACGAAAGGACGAAACACAGAACAACTACCAACTCC114180.10406112720857622No Hit
ATACACGAACTCTAGTGGAAAGGACGAAACACAGAACAACTACCAACTCC114000.10389707918880442No Hit
ATAAACTAACTCTACTGGAAAGGACGAAACACAGAACAACTACCAACTCC113530.10346873158162251No Hit
ATACACAATCTCTACTCGAAAGGACGAAACACAGAACAACTACCAACTCC112860.10285810839691636No Hit
ATACACAATCTCTAGACGAAAGGACGAAACACAGAACAACTACCAACTCC112710.1027214017137732No Hit
ATAAACTAACTCTTCACGAAAGGACGAAACACAGAACAACTACCAACTCC112210.10226571276996266No Hit
ATACACAAACTCTAGTCGAAAGGACGAAACACAGAACAACTACCAACTCC110390.10060700501449227No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)