FastQCFastQC Report
Mon 21 Nov 2022
H3TJ5BGXN_n01_R17_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3TJ5BGXN_n01_R17_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9741540
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACGATCTCTACACGAAAGGACGAAACACAGAACAACTACCAACTCC6113416.275609400567056No Hit
ATAAACAAACTCTACACGAAAGGACGAAACACAGAACAACTACCAACTCC3064153.145447229082876No Hit
ATACACGATCTCTACTCGAAAGGACGAAACACAGAACAACTACCAACTCC2699262.770876062716983No Hit
ATACACGATCTCTAGACGAAAGGACGAAACACAGAACAACTACCAACTCC2357772.420325739051526No Hit
ATACACGATCTCTACAGGAAAGGACGAAACACAGAACAACTACCAACTCC1839291.8880895628411936No Hit
ATAAACTAACTCTACACGAAAGGACGAAACACAGAACAACTACCAACTCC1400731.4378938032385022No Hit
ATACACTATCTCTACACGAAAGGACGAAACACAGAACAACTACCAACTCC1388621.4254625038751574No Hit
ATACACGATCTCTAGTCGAAAGGACGAAACACAGAACAACTACCAACTCC1215031.2472668592440208No Hit
ATACACGATCTCTAGAGGAAAGGACGAAACACAGAACAACTACCAACTCC1193401.2250629777222082No Hit
ATACACAAACTCTACACGAAAGGACGAAACACAGAACAACTACCAACTCC1180941.212272392250096No Hit
ATAAACGATCTCTACACGAAAGGACGAAACACAGAACAACTACCAACTCC1168251.1992457044779368No Hit
ATACACGAACTCTACACGAAAGGACGAAACACAGAACAACTACCAACTCC1118111.1477754030676874No Hit
ATAAACCAACTCTACACGAAAGGACGAAACACAGAACAACTACCAACTCC984631.0107539465012718No Hit
ATACACGATCTCTACACGAAAGGACGAAACACAGAACACCTACCAACTCC953950.9792599527384788No Hit
ATAAACTATCTCTACACGAAAGGACGAAACACAGAACAACTACCAACTCC934900.9597045231041499No Hit
ATACACGATCTCTACTGGAAAGGACGAAACACAGAACAACTACCAACTCC911520.9357042110385011No Hit
ATACACTAACTCTACACGAAAGGACGAAACACAGAACAACTACCAACTCC895510.9192694378917501No Hit
ATACACCAACTCTACACGAAAGGACGAAACACAGAACAACTACCAACTCC831170.8532223857829461No Hit
ATAAACGAACTCTACACGAAAGGACGAAACACAGAACAACTACCAACTCC826920.848859625890773No Hit
ATAAACAAACTCTACTCGAAAGGACGAAACACAGAACAACTACCAACTCC740420.7600646304383085No Hit
ATACACGATCTCTAGTGGAAAGGACGAAACACAGAACAACTACCAACTCC725870.7451285936309865No Hit
ATAAACAAACTCTAGACGAAAGGACGAAACACAGAACAACTACCAACTCC695570.7140246819291406No Hit
ATAAACAATCTCTACACGAAAGGACGAAACACAGAACAACTACCAACTCC609670.6258456055202771No Hit
ATAAACAAACTCTACACGAAAGGACGAAACACAGAACACCTACCAACTCC527020.5410027572642518No Hit
ATAAACAAACTCTACAGGAAAGGACGAAACACAGAACAACTACCAACTCC526360.5403252463162909No Hit
ATACACGATCTCTACACGAAAGGACGAAACACAGAACCACTACCAACTCC503580.516940853294243No Hit
ATAAACGATCTCTACTCGAAAGGACGAAACACAGAACAACTACCAACTCC496570.5097448658015058No Hit
ATACACTATCTCTACTCGAAAGGACGAAACACAGAACAACTACCAACTCC494880.5080100271620298No Hit
ATACACGATCTCTACTCGAAAGGACGAAACACAGAACACCTACCAACTCC469040.48148444701761733No Hit
ATACACTATCTCTAGACGAAAGGACGAAACACAGAACAACTACCAACTCC457620.4697614545544133No Hit
ATACACGAACTCTACTCGAAAGGACGAAACACAGAACAACTACCAACTCC449240.4611591185787874No Hit
ATAAACGATCTCTAGACGAAAGGACGAAACACAGAACAACTACCAACTCC440370.45205378205088725No Hit
ATAAACTAACTCTACTCGAAAGGACGAAACACAGAACAACTACCAACTCC429230.440618218474697No Hit
ATACACGAACTCTAGACGAAAGGACGAAACACAGAACAACTACCAACTCC410350.42123729923605513No Hit
ATACACGATCTCTAGACGAAAGGACGAAACACAGAACACCTACCAACTCC409060.41991307329231314No Hit
ATAAACAAACTCTACACGAAAGGACGAAACACAGAACAACTACCAACTGC407450.41826035719198407No Hit
ATAAACTAACTCTAGACGAAAGGACGAAACACAGAACAACTACCAACTCC394990.4054697717198718No Hit
ATACACTATCTCTACAGGAAAGGACGAAACACAGAACAACTACCAACTCC381200.3913138990344443No Hit
ATAAACGATCTCTACAGGAAAGGACGAAACACAGAACAACTACCAACTCC363110.37274393986987686No Hit
ATACACGATCTCTACAGGAAAGGACGAAACACAGAACACCTACCAACTCC357600.3670877499861418No Hit
ATAAACTATCTCTACTCGAAAGGACGAAACACAGAACAACTACCAACTCC342360.35144340627867876No Hit
ATAAACTATCTCTAGACGAAAGGACGAAACACAGAACAACTACCAACTCC314170.3225054765468294No Hit
ATACACAAACTCTACTCGAAAGGACGAAACACAGAACAACTACCAACTCC313710.3220332719467353No Hit
ATAAACGAACTCTACTCGAAAGGACGAAACACAGAACAACTACCAACTCC311660.31992888188109886No Hit
ATACACGAACTCTACAGGAAAGGACGAAACACAGAACAACTACCAACTCC307230.31538134627584546No Hit
ATAAACTAACTCTACAGGAAAGGACGAAACACAGAACAACTACCAACTCC303670.3117268932838134No Hit
ATACACAAACTCTAGACGAAAGGACGAAACACAGAACAACTACCAACTCC300930.30891419631803596No Hit
ATACACTAACTCTACTCGAAAGGACGAAACACAGAACAACTACCAACTCC297530.3054239884042975No Hit
ATAAACCAACTCTACTCGAAAGGACGAAACACAGAACAACTACCAACTCC291480.29921347138132165No Hit
ATACACTAACTCTAGACGAAAGGACGAAACACAGAACAACTACCAACTCC284080.29161713651024374No Hit
ATAAACGAACTCTAGACGAAAGGACGAAACACAGAACAACTACCAACTCC280690.28813719391389864No Hit
ATACACAATCTCTACACGAAAGGACGAAACACAGAACAACTACCAACTCC264630.27165109418018096No Hit
ATAAACCAACTCTAGACGAAAGGACGAAACACAGAACAACTACCAACTCC262700.26966988792326474No Hit
ATACACCAACTCTACTCGAAAGGACGAAACACAGAACAACTACCAACTCC260300.26720621174886106No Hit
ATAAACTATCTCTACAGGAAAGGACGAAACACAGAACAACTACCAACTCC260100.26700090540099414No Hit
ATACACGATCTCTAGAGGAAAGGACGAAACACAGAACACCTACCAACTCC254210.26095463345631187No Hit
ATACACCATCTCTACACGAAAGGACGAAACACAGAACAACTACCAACTCC245160.25166452121533145No Hit
ATACACCAACTCTAGACGAAAGGACGAAACACAGAACAACTACCAACTCC243550.2500118051150023No Hit
ATACACGATCTCTAGTCGAAAGGACGAAACACAGAACACCTACCAACTCC242320.24874917107562047No Hit
ATAAACCATCTCTACACGAAAGGACGAAACACAGAACAACTACCAACTCC235690.24194326564383042No Hit
ATACACGATCTCTACTCGAAAGGACGAAACACAGAACCACTACCAACTCC235490.24173795929596348No Hit
ATAAACGATCTCTAGAGGAAAGGACGAAACACAGAACAACTACCAACTCC233750.23995179406952083No Hit
CTAAACAAACTCTACACGAAAGGACGAAACACAGAACAACTACCAACTCC232100.23825801669961835No Hit
ATAAACTAACTCTACACGAAAGGACGAAACACAGAACACCTACCAACTCC232070.23822722074743832No Hit
ATAAACGATCTCTAGTCGAAAGGACGAAACACAGAACAACTACCAACTCC225270.23124680491996133No Hit
ATACACAAACTCTACAGGAAAGGACGAAACACAGAACAACTACCAACTCC223620.2295530275500588No Hit
ATACACAAACTCTACACGAAAGGACGAAACACAGAACACCTACCAACTCC221530.22740757621484897No Hit
ATACACGAACTCTACACGAAAGGACGAAACACAGAACACCTACCAACTCC216910.22266499957912197No Hit
ATACACTATCTCTAGAGGAAAGGACGAAACACAGAACAACTACCAACTCC216740.22249048918343506No Hit
ATAAACGAACTCTACAGGAAAGGACGAAACACAGAACAACTACCAACTCC216480.222223590931208No Hit
ATACACTAACTCTACAGGAAAGGACGAAACACAGAACAACTACCAACTCC214180.21986256793073786No Hit
ATAAACAAACTCTACACGAAAGGACGAAACACAGAACCACTACCAACTCC207950.21346727519468175No Hit
ATACACTATCTCTACACGAAAGGACGAAACACAGAACACCTACCAACTCC206520.2119993348074329No Hit
ATACACGATCTCTAGACGAAAGGACGAAACACAGAACCACTACCAACTCC202970.20835514713279418No Hit
ATACACGATCTCTACTGGAAAGGACGAAACACAGAACACCTACCAACTCC199760.20505998024952934No Hit
ATAAACAATCTCTACTCGAAAGGACGAAACACAGAACAACTACCAACTCC198730.20400265255801445No Hit
ATACACGAACTCTAGTCGAAAGGACGAAACACAGAACAACTACCAACTCC195130.200307138296409No Hit
ATACACGATCTCTACACGAAAGGACGAAACACCGAACAACTACCAACTCC194930.200101831948542No Hit
ATACACTATCTCTAGTCGAAAGGACGAAACACAGAACAACTACCAACTCC194760.1999273215528551No Hit
ATAAACTAACTCTACACGAAAGGACGAAACACAGAACAACTACCAACTGC194360.19951670885712114No Hit
ATAAACAATCTCTAGACGAAAGGACGAAACACAGAACAACTACCAACTCC194150.19930113719186082No Hit
ATAAACCAACTCTACAGGAAAGGACGAAACACAGAACAACTACCAACTCC192580.19768948236110512No Hit
ATAAACAAACTCTAGAGGAAAGGACGAAACACAGAACAACTACCAACTCC192100.1971967471262244No Hit
ATACACAAACTCTACACGAAAGGACGAAACACAGAACAACTACCAACTGC192080.1971762164914377No Hit
ATAAACAAACTCTAGTCGAAAGGACGAAACACAGAACAACTACCAACTCC187980.19296743636016483No Hit
ATACACGAACTCTAGAGGAAAGGACGAAACACAGAACAACTACCAACTCC184710.18961067757253985No Hit
ATAAACGATCTCTACACGAAAGGACGAAACACAGAACACCTACCAACTCC183790.18866626837235181No Hit
ATAAACGATCTCTACTGGAAAGGACGAAACACAGAACAACTACCAACTCC180810.18560720378913398No Hit
ATACACGATCTCTAGTGGAAAGGACGAAACACAGAACACCTACCAACTCC180690.18548401998041378No Hit
ATACACCAACTCTACAGGAAAGGACGAAACACAGAACAACTACCAACTCC177270.1819732814318886No Hit
ATAAACCAACTCTACACGAAAGGACGAAACACAGAACACCTACCAACTCC170550.1750749881435584No Hit
ATACACGAACTCTACACGAAAGGACGAAACACAGAACAACTACCAACTGC169110.17359678243891624No Hit
ATACACTAACTCTACACGAAAGGACGAAACACAGAACACCTACCAACTCC166160.17056851380787844No Hit
ATAAACAAACTCTTCACGAAAGGACGAAACACAGAACAACTACCAACTCC165580.16997312539906423No Hit
ATAAACAAACTCTACACGAAAGGACGAAACACAGAACAACTACCAACTCG164150.16850518501181538No Hit
ATACACGATCTCTACAGGAAAGGACGAAACACAGAACCACTACCAACTCC160240.16449144591101614No Hit
ATACACCAACTCTACACGAAAGGACGAAACACAGAACACCTACCAACTCC159890.16413215980224893No Hit
ATACACTATCTCTACTGGAAAGGACGAAACACAGAACAACTACCAACTCC156600.16075487037983727No Hit
ATAAACTATCTCTAGAGGAAAGGACGAAACACAGAACAACTACCAACTCC148670.15261447368691192No Hit
ATAAACGATCTCTAGTGGAAAGGACGAAACACAGAACAACTACCAACTCC144960.14880604093397964No Hit
ATAAACAATCTCTACAGGAAAGGACGAAACACAGAACAACTACCAACTCC144060.14788216236857826No Hit
ATACACGAACTCTACTGGAAAGGACGAAACACAGAACAACTACCAACTCC143390.14719438610322394No Hit
ATAAACGAACTCTACACGAAAGGACGAAACACAGAACACCTACCAACTCC140950.1446896486592469No Hit
ATAAACTAACTCTAGTCGAAAGGACGAAACACAGAACAACTACCAACTCC140150.14386842326777902No Hit
ATAAACCAACTCTACACGAAAGGACGAAACACAGAACAACTACCAACTGC140030.14374523945905884No Hit
ATAAACAAACTCTACTGGAAAGGACGAAACACAGAACAACTACCAACTCC139930.14364258628512533No Hit
ATACACTAACTCTACACGAAAGGACGAAACACAGAACAACTACCAACTGC139560.14326276954157144No Hit
ATAAACTATCTCTAGTCGAAAGGACGAAACACAGAACAACTACCAACTCC138550.14222597248484325No Hit
ATAAACTATCTCTACACGAAAGGACGAAACACAGAACACCTACCAACTCC138490.14216438058048317No Hit
ATAAACTAACTCTAGAGGAAAGGACGAAACACAGAACAACTACCAACTCC137140.14077856273238112No Hit
ATACACGATCTCTACACGAAAGGACGAAACACAGAACAACTACCAACTGC133860.13741153862736283No Hit
ATAAACAAACTCTACTCGAAAGGACGAAACACAGAACACCTACCAACTCC132370.1358820063357539No Hit
ATACACCAACTCTACACGAAAGGACGAAACACAGAACAACTACCAACTGC131740.13523529133997295No Hit
ATAAACGAACTCTAGTCGAAAGGACGAAACACAGAACAACTACCAACTCC124400.12770054837325515No Hit
ATAAACAAACTCTAGACGAAAGGACGAAACACAGAACACCTACCAACTCC121030.12424113641169672No Hit
ATAAACGAACTCTACACGAAAGGACGAAACACAGAACAACTACCAACTGC117030.12013500945435732No Hit
ATACACGAACTCTTCACGAAAGGACGAAACACAGAACAACTACCAACTCC116780.11987837651952361No Hit
ATACACGATCTCTAGTCGAAAGGACGAAACACAGAACCACTACCAACTCC114980.11803061938872086No Hit
ATAAACAAACTCTACTCGAAAGGACGAAACACAGAACAACTACCAACTGC114010.11703488360156608No Hit
ATACACTATCTCTACACGAAAGGACGAAACACAGAACCACTACCAACTCC113780.11679878130151906No Hit
ATACACTATCTCTAGTGGAAAGGACGAAACACAGAACAACTACCAACTCC112530.1155156166273505No Hit
ATACACGATCTCTAGAGGAAAGGACGAAACACAGAACCACTACCAACTCC112120.11509473861422322No Hit
ATAAACGAACTCTAGAGGAAAGGACGAAACACAGAACAACTACCAACTCC111860.11482784036199614No Hit
ATAAACTATCTCTACTGGAAAGGACGAAACACAGAACAACTACCAACTCC110830.11377051267048126No Hit
ATAAACAATCTCTACACGAAAGGACGAAACACAGAACACCTACCAACTCC107120.10996207991754896No Hit
ATACACTAACTCTAGTCGAAAGGACGAAACACAGAACAACTACCAACTCC106750.10958226317399507No Hit
ATACACTAACTCTAGAGGAAAGGACGAAACACAGAACAACTACCAACTCC105960.10877130309992056No Hit
ATAAACTAACTCTACTGGAAAGGACGAAACACAGAACAACTACCAACTCC103620.106369218829877No Hit
ATACACCATCTCTACTCGAAAGGACGAAACACAGAACAACTACCAACTCC103010.10574303446888274No Hit
ATACACGAACTCTAGTGGAAAGGACGAAACACAGAACAACTACCAACTCC102890.10561985066016256No Hit
ATAAACTAACTCTTCACGAAAGGACGAAACACAGAACAACTACCAACTCC102520.10524003391660867No Hit
ATAAACAAACTCTAGACGAAAGGACGAAACACAGAACAACTACCAACTGC101850.10455225765125431No Hit
ATAAACAAACTCTACAGGAAAGGACGAAACACAGAACACCTACCAACTCC100160.10281741901177843No Hit
ATACACCATCTCTAGACGAAAGGACGAAACACAGAACAACTACCAACTCC97780.10037427347216149No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)