FastQCFastQC Report
Mon 21 Nov 2022
H3TJ5BGXN_n01_R16_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3TJ5BGXN_n01_R16_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14137207
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACGATCTCTACACGAAAGGACGAAACACAGAACAACTACCAACTCC8901876.2967671054119805No Hit
ATAAACAAACTCTACACGAAAGGACGAAACACAGAACAACTACCAACTCC4667993.3019181228654286No Hit
ATACACGATCTCTACTCGAAAGGACGAAACACAGAACAACTACCAACTCC3860902.731020349351891No Hit
ATACACGATCTCTAGACGAAAGGACGAAACACAGAACAACTACCAACTCC3338572.3615485010582358No Hit
ATACACGATCTCTACAGGAAAGGACGAAACACAGAACAACTACCAACTCC2609681.8459657554706528No Hit
ATACACTATCTCTACACGAAAGGACGAAACACAGAACAACTACCAACTCC2083891.4740464647649285No Hit
ATAAACTAACTCTACACGAAAGGACGAAACACAGAACAACTACCAACTCC2051271.4509726001748435No Hit
ATACACAAACTCTACACGAAAGGACGAAACACAGAACAACTACCAACTCC1855691.3126284420960943No Hit
ATAAACGATCTCTACACGAAAGGACGAAACACAGAACAACTACCAACTCC1704691.205818094054929No Hit
ATACACGATCTCTAGTCGAAAGGACGAAACACAGAACAACTACCAACTCC1681121.1891457768143312No Hit
ATACACGATCTCTAGAGGAAAGGACGAAACACAGAACAACTACCAACTCC1644221.1630444401075828No Hit
ATACACGAACTCTACACGAAAGGACGAAACACAGAACAACTACCAACTCC1642481.1618136453685655No Hit
ATAAACCAACTCTACACGAAAGGACGAAACACAGAACAACTACCAACTCC1477161.0448739980959463No Hit
ATAAACTATCTCTACACGAAAGGACGAAACACAGAACAACTACCAACTCC1381520.9772227286478865No Hit
ATACACGATCTCTACACGAAAGGACGAAACACAGAACACCTACCAACTCC1374750.9724339468184912No Hit
ATACACTAACTCTACACGAAAGGACGAAACACAGAACAACTACCAACTCC1348010.953519319622327No Hit
ATACACGATCTCTACTGGAAAGGACGAAACACAGAACAACTACCAACTCC1276620.9030213676576992No Hit
ATACACCAACTCTACACGAAAGGACGAAACACAGAACAACTACCAACTCC1267930.8968744674955951No Hit
ATAAACGAACTCTACACGAAAGGACGAAACACAGAACAACTACCAACTCC1205890.8529902688699402No Hit
ATAAACAAACTCTACTCGAAAGGACGAAACACAGAACAACTACCAACTCC1118070.7908705022144756No Hit
ATAAACAAACTCTAGACGAAAGGACGAAACACAGAACAACTACCAACTCC1031190.7294156476593997No Hit
ATACACGATCTCTAGTGGAAAGGACGAAACACAGAACAACTACCAACTCC984340.6962761456347071No Hit
ATAAACAATCTCTACACGAAAGGACGAAACACAGAACAACTACCAACTCC924600.6540188595951095No Hit
ATAAACAAACTCTACAGGAAAGGACGAAACACAGAACAACTACCAACTCC789730.5586181202552951No Hit
ATAAACAAACTCTACACGAAAGGACGAAACACAGAACACCTACCAACTCC788200.5575358697089178No Hit
ATACACTATCTCTACTCGAAAGGACGAAACACAGAACAACTACCAACTCC727450.5145641568380516No Hit
ATACACGATCTCTACACGAAAGGACGAAACACAGAACCACTACCAACTCC724060.5121662291568624No Hit
ATAAACGATCTCTACTCGAAAGGACGAAACACAGAACAACTACCAACTCC713730.5048592695855695No Hit
ATACACTATCTCTAGACGAAAGGACGAAACACAGAACAACTACCAACTCC665850.47099119366364234No Hit
ATACACGATCTCTACTCGAAAGGACGAAACACAGAACACCTACCAACTCC662860.4688762073017676No Hit
ATACACGAACTCTACTCGAAAGGACGAAACACAGAACAACTACCAACTCC649610.4595037760994799No Hit
ATAAACGATCTCTAGACGAAAGGACGAAACACAGAACAACTACCAACTCC621210.4394149424281614No Hit
ATAAACTAACTCTACTCGAAAGGACGAAACACAGAACAACTACCAACTCC620550.4389480892512927No Hit
ATAAACAAACTCTACACGAAAGGACGAAACACAGAACAACTACCAACTGC613920.43425833688365745No Hit
ATACACGAACTCTAGACGAAAGGACGAAACACAGAACAACTACCAACTCC588300.41613594538157356No Hit
ATACACGATCTCTAGACGAAAGGACGAAACACAGAACACCTACCAACTCC576180.407562823406349No Hit
ATAAACTAACTCTAGACGAAAGGACGAAACACAGAACAACTACCAACTCC571770.40444339536090823No Hit
ATACACTATCTCTACAGGAAAGGACGAAACACAGAACAACTACCAACTCC564370.39920898095359286No Hit
ATAAACGATCTCTACAGGAAAGGACGAAACACAGAACAACTACCAACTCC521440.36884230385818073No Hit
ATACACGATCTCTACAGGAAAGGACGAAACACAGAACACCTACCAACTCC502070.35514087047038356No Hit
ATAAACTATCTCTACTCGAAAGGACGAAACACAGAACAACTACCAACTCC497710.3520568100898572No Hit
ATACACAAACTCTACTCGAAAGGACGAAACACAGAACAACTACCAACTCC479160.3389354064066544No Hit
ATACACAAACTCTAGACGAAAGGACGAAACACAGAACAACTACCAACTCC460250.3255593555360688No Hit
ATAAACTATCTCTAGACGAAAGGACGAAACACAGAACAACTACCAACTCC455500.3221994273692109No Hit
ATACACGAACTCTACAGGAAAGGACGAAACACAGAACAACTACCAACTCC446930.31613740960290104No Hit
ATAAACGAACTCTACTCGAAAGGACGAAACACAGAACAACTACCAACTCC444420.31436195282420354No Hit
ATAAACTAACTCTACAGGAAAGGACGAAACACAGAACAACTACCAACTCC440820.3118154809503744No Hit
ATACACTAACTCTACTCGAAAGGACGAAACACAGAACAACTACCAACTCC436670.3088799647624881No Hit
ATAAACCAACTCTACTCGAAAGGACGAAACACAGAACAACTACCAACTCC429250.30363140328920696No Hit
ATACACTAACTCTAGACGAAAGGACGAAACACAGAACAACTACCAACTCC416130.29435092801569646No Hit
ATACACAATCTCTACACGAAAGGACGAAACACAGAACAACTACCAACTCC413360.2923915593794446No Hit
ATACACCAACTCTACTCGAAAGGACGAAACACAGAACAACTACCAACTCC401710.2841508934544143No Hit
ATAAACGAACTCTAGACGAAAGGACGAAACACAGAACAACTACCAACTCC394780.27924893509729326No Hit
ATAAACCAACTCTAGACGAAAGGACGAAACACAGAACAACTACCAACTCC389300.27537263902268677No Hit
ATACACCATCTCTACACGAAAGGACGAAACACAGAACAACTACCAACTCC381980.27019481287923425No Hit
ATAAACTATCTCTACAGGAAAGGACGAAACACAGAACAACTACCAACTCC376510.26632559033761055No Hit
ATACACCAACTCTAGACGAAAGGACGAAACACAGAACAACTACCAACTCC366310.2591105866950947No Hit
ATAAACCATCTCTACACGAAAGGACGAAACACAGAACAACTACCAACTCC356680.2522987744326019No Hit
ATACACGATCTCTAGAGGAAAGGACGAAACACAGAACACCTACCAACTCC348810.24673190397509212No Hit
ATACACAAACTCTACACGAAAGGACGAAACACAGAACACCTACCAACTCC344650.24378931425422293No Hit
ATACACAAACTCTACAGGAAAGGACGAAACACAGAACAACTACCAACTCC343250.24279901963662273No Hit
ATAAACTAACTCTACACGAAAGGACGAAACACAGAACACCTACCAACTCC334670.23672992833732998No Hit
CTAAACAAACTCTACACGAAAGGACGAAACACAGAACAACTACCAACTCC334390.23653186941380994No Hit
ATACACGATCTCTAGTCGAAAGGACGAAACACAGAACACCTACCAACTCC334180.23638332522116992No Hit
ATACACGATCTCTACTCGAAAGGACGAAACACAGAACCACTACCAACTCC332070.23489080976178675No Hit
ATAAACGATCTCTAGAGGAAAGGACGAAACACAGAACAACTACCAACTCC325750.2304203369166201No Hit
ATAAACGATCTCTAGTCGAAAGGACGAAACACAGAACAACTACCAACTCC318880.2255608197573962No Hit
ATACACTAACTCTACAGGAAAGGACGAAACACAGAACAACTACCAACTCC316750.2240541572320473No Hit
ATACACGAACTCTACACGAAAGGACGAAACACAGAACACCTACCAACTCC316590.22394098070432156No Hit
ATAAACAAACTCTACACGAAAGGACGAAACACAGAACCACTACCAACTCC312140.22079325852694948No Hit
ATACACTATCTCTAGAGGAAAGGACGAAACACAGAACAACTACCAACTCC310400.21956246378793207No Hit
ATAAACGAACTCTACAGGAAAGGACGAAACACAGAACAACTACCAACTCC307610.21758894808571452No Hit
ATACACTATCTCTACACGAAAGGACGAAACACAGAACACCTACCAACTCC305170.215863006037897No Hit
ATACACAAACTCTACACGAAAGGACGAAACACAGAACAACTACCAACTGC300710.21270821032754206No Hit
ATAAACAATCTCTACTCGAAAGGACGAAACACAGAACAACTACCAACTCC298780.21134301846185036No Hit
ATAAACCAACTCTACAGGAAAGGACGAAACACAGAACAACTACCAACTCC288580.20412801481933454No Hit
ATAAACAATCTCTAGACGAAAGGACGAAACACAGAACAACTACCAACTCC287860.2036187204445687No Hit
ATACACGATCTCTAGACGAAAGGACGAAACACAGAACCACTACCAACTCC284050.2009237043780996No Hit
ATAAACAAACTCTAGTCGAAAGGACGAAACACAGAACAACTACCAACTCC282850.20007488042015653No Hit
ATAAACAAACTCTAGAGGAAAGGACGAAACACAGAACAACTACCAACTCC282190.19960802724328786No Hit
ATACACTATCTCTAGTCGAAAGGACGAAACACAGAACAACTACCAACTCC280800.19862480615867054No Hit
ATACACGAACTCTAGTCGAAAGGACGAAACACAGAACAACTACCAACTCC279560.19774768806879606No Hit
ATAAACTAACTCTACACGAAAGGACGAAACACAGAACAACTACCAACTGC279060.19739401141965313No Hit
ATACACGATCTCTACTGGAAAGGACGAAACACAGAACACCTACCAACTCC278730.1971605848312188No Hit
ATACACGATCTCTACACGAAAGGACGAAACACCGAACAACTACCAACTCC276840.19582368709745848No Hit
ATACACCAACTCTACAGGAAAGGACGAAACACAGAACAACTACCAACTCC269240.19044780203048595No Hit
ATAAACGATCTCTACACGAAAGGACGAAACACAGAACACCTACCAACTCC266740.1886794187847713No Hit
ATACACGAACTCTAGAGGAAAGGACGAAACACAGAACAACTACCAACTCC259060.18324694545393583No Hit
ATAAACGATCTCTACTGGAAAGGACGAAACACAGAACAACTACCAACTCC254590.18008507621059805No Hit
ATAAACAAACTCTTCACGAAAGGACGAAACACAGAACAACTACCAACTCC249440.17644220672442584No Hit
ATAAACCAACTCTACACGAAAGGACGAAACACAGAACACCTACCAACTCC249320.17635732432863155No Hit
ATAAACAAACTCTACACGAAAGGACGAAACACAGAACAACTACCAACTCG247870.17533166204611703No Hit
ATACACGATCTCTAGTGGAAAGGACGAAACACAGAACACCTACCAACTCC247130.1748082206053855No Hit
ATACACCAACTCTACACGAAAGGACGAAACACAGAACACCTACCAACTCC246280.1742069703018425No Hit
ATACACTAACTCTACACGAAAGGACGAAACACAGAACACCTACCAACTCC245820.173881587784631No Hit
ATACACGAACTCTACACGAAAGGACGAAACACAGAACAACTACCAACTGC244300.17280641077123648No Hit
ATACACTATCTCTACTGGAAAGGACGAAACACAGAACAACTACCAACTCC228660.16174340518604557No Hit
ATACACGATCTCTACAGGAAAGGACGAAACACAGAACCACTACCAACTCC223810.15831274168935913No Hit
ATAAACAATCTCTACAGGAAAGGACGAAACACAGAACAACTACCAACTCC217250.15367250405260388No Hit
ATAAACTATCTCTAGAGGAAAGGACGAAACACAGAACAACTACCAACTCC214210.15152215002581487No Hit
ATAAACGAACTCTACACGAAAGGACGAAACACAGAACACCTACCAACTCC208630.14757511862137973No Hit
ATAAACCAACTCTACACGAAAGGACGAAACACAGAACAACTACCAACTGC208150.14723558903820252No Hit
ATAAACAAACTCTACTGGAAAGGACGAAACACAGAACAACTACCAACTCC207520.14678995646028242No Hit
ATAAACTATCTCTACACGAAAGGACGAAACACAGAACACCTACCAACTCC206950.1463867650802595No Hit
ATACACTAACTCTACACGAAAGGACGAAACACAGAACAACTACCAACTGC206090.14577844124373365No Hit
ATACACGAACTCTACTGGAAAGGACGAAACACAGAACAACTACCAACTCC202770.14343002829342458No Hit
ATACACCAACTCTACACGAAAGGACGAAACACAGAACAACTACCAACTGC200720.14197995403193855No Hit
ATAAACGATCTCTAGTGGAAAGGACGAAACACAGAACAACTACCAACTCC198080.14011254132446388No Hit
ATAAACTAACTCTAGTCGAAAGGACGAAACACAGAACAACTACCAACTCC197650.13980837940620094No Hit
ATAAACAAACTCTACTCGAAAGGACGAAACACAGAACACCTACCAACTCC196700.13913639377282938No Hit
ATAAACTAACTCTAGAGGAAAGGACGAAACACAGAACAACTACCAACTCC196040.1386695405959607No Hit
ATAAACTATCTCTAGTCGAAAGGACGAAACACAGAACAACTACCAACTCC193090.13658284836601742No Hit
ATACACGATCTCTACACGAAAGGACGAAACACAGAACAACTACCAACTGC189280.13388783229954826No Hit
ATAAACAAACTCTAGACGAAAGGACGAAACACAGAACACCTACCAACTCC181580.12844121190274713No Hit
ATAAACGAACTCTAGTCGAAAGGACGAAACACAGAACAACTACCAACTCC173710.12287434144523737No Hit
ATACACTATCTCTACACGAAAGGACGAAACACAGAACCACTACCAACTCC169750.12007322238402536No Hit
ATAAACAAACTCTACTCGAAAGGACGAAACACAGAACAACTACCAACTGC168950.11950733974539667No Hit
ATAAACGAACTCTACACGAAAGGACGAAACACAGAACAACTACCAACTGC168710.11933757495380806No Hit
ATACACGAACTCTTCACGAAAGGACGAAACACAGAACAACTACCAACTCC168420.11913244249730516No Hit
ATACACGATCTCTAGTCGAAAGGACGAAACACAGAACCACTACCAACTCC161990.11458416078932705No Hit
ATAAACAATCTCTACACGAAAGGACGAAACACAGAACACCTACCAACTCC160570.11357971910576113No Hit
ATAAACGAACTCTAGAGGAAAGGACGAAACACAGAACAACTACCAACTCC158690.1122498949049837No Hit
ATAAACTATCTCTACTGGAAAGGACGAAACACAGAACAACTACCAACTCC157600.1114788798098521No Hit
ATACACTAACTCTAGTCGAAAGGACGAAACACAGAACAACTACCAACTCC157530.11142936507897211No Hit
ATACACTATCTCTAGTGGAAAGGACGAAACACAGAACAACTACCAACTCC156410.11063712938489195No Hit
ATACACCATCTCTACTCGAAAGGACGAAACACAGAACAACTACCAACTCC156090.11041077632944046No Hit
ATACACGATCTCTAGAGGAAAGGACGAAACACAGAACCACTACCAACTCC154680.1094134081788574No Hit
ATACACTAACTCTAGAGGAAAGGACGAAACACAGAACAACTACCAACTCC152290.10772283379595418No Hit
ATAAACAAACTCTAGACGAAAGGACGAAACACAGAACAACTACCAACTGC151850.1074115983447084No Hit
ATAAACAAACTCTACAGGAAAGGACGAAACACAGAACACCTACCAACTCC148970.10537442084564512No Hit
ATAAACTAACTCTACTGGAAAGGACGAAACACAGAACAACTACCAACTCC147390.10425680263435345No Hit
ATAAACTAACTCTTCACGAAAGGACGAAACACAGAACAACTACCAACTCC146620.10371214059467333No Hit
ATACACCATCTCTAGACGAAAGGACGAAACACAGAACAACTACCAACTCC146140.10337261101149613No Hit
ATACACGAACTCTAGTGGAAAGGACGAAACACAGAACAACTACCAACTCC142580.10085443326959843No Hit
ATACACAATCTCTACTCGAAAGGACGAAACACAGAACAACTACCAACTCC142310.10066344787906126No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)