FastQCFastQC Report
Mon 21 Nov 2022
H3TJ5BGXN_n01_R15_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3TJ5BGXN_n01_R15_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11513997
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGTAGACTCTTCACGAAAGGACGAAACACCGAACCACTACCAACTGCT4072803.5372599106982574No Hit
TAAGTAGACTCTTCACGAAAGGACGAAACACCGAACCAATACCAACTGCT3525893.0622641294764974No Hit
TAAGTAGACTCTTCAGGAAAGGACGAAACACCGAACCACTACCAACTGCT2476392.150764847341892No Hit
TAAGTAGACTCTTCTCGAAAGGACGAAACACCGAACCACTACCAACTGCT2319592.0145827726027723No Hit
TAAGTAGACTCTTCAGGAAAGGACGAAACACCGAACCAATACCAACTGCT2011651.7471343791387126No Hit
TAAGTAGACTCTTCACCAAAGGACGAAACACCGAACCACTACCAACTGCT1982201.721556814718642No Hit
TAAGTAGACTCTTCACCAAAGGACGAAACACCGAACCAATACCAACTGCT1968911.7100143416747462No Hit
TAAGTAGACTCTTCTCGAAAGGACGAAACACCGAACCAATACCAACTGCT1897151.6476901982864853No Hit
TAAGTAGACTCTTCTGGAAAGGACGAAACACCGAACCACTACCAACTGCT1637651.4223123386257615No Hit
TAAGTAGAGTCTTCACGAAAGGACGAAACACCGAACCACTACCAACTGCT1245731.0819266324283392No Hit
TAAGTAGACTCTTCTGGAAAGGACGAAACACCGAACCAATACCAACTGCT1231691.0697327782871577No Hit
TAAGTAGACTCTTCAGCAAAGGACGAAACACCGAACCACTACCAACTGCT1110090.964122189713963No Hit
TAAGTAGAGTCTTCAGGAAAGGACGAAACACCGAACCACTACCAACTGCT1060120.9207228384721657No Hit
TAAGTAGACTCTTCAGCAAAGGACGAAACACCGAACCAATACCAACTGCT1053000.9145390605886036No Hit
TAAGTAGAGTCTTCACGAAAGGACGAAACACCGAACCAATACCAACTGCT1034510.8984803452701959No Hit
TAAGTAGAGTCTTCTGGAAAGGACGAAACACCGAACCACTACCAACTGCT1017840.8840023147478673No Hit
TAAGTAGAGTCTTCTCGAAAGGACGAAACACCGAACCACTACCAACTGCT996070.8650948927639984No Hit
TAAGTAGAGTCTTCAGGAAAGGACGAAACACCGAACCAATACCAACTGCT815340.7081294184808282No Hit
TAAGTAGAGTCTTCTCGAAAGGACGAAACACCGAACCAATACCAACTGCT754450.6552459584625565No Hit
TAAGTACACTCTTCACGAAAGGACGAAACACCGAACCAATACCAACTGCT752180.6532744450081062No Hit
TAAGTACACTCTTCACGAAAGGACGAAACACCGAACCACTACCAACTGCT738980.6418101376958845No Hit
TAAGTAGAGTCTTCTGGAAAGGACGAAACACCGAACCAATACCAACTGCT713570.6197413461198574No Hit
TAAGTAGACTCTTCTCCAAAGGACGAAACACCGAACCACTACCAACTGCT711080.6175787608768701No Hit
TAAGTAGACTCTTCTCCAAAGGACGAAACACCGAACCAATACCAACTGCT671090.5828471207696163No Hit
TAAGTAGAGTCTTCACCAAAGGACGAAACACCGAACCACTACCAACTGCT554080.48122298451180767No Hit
TAAGTACACTCTTCACCAAAGGACGAAACACCGAACCAATACCAACTGCT553490.4807105647152765No Hit
TAAGTAGAGTCTTCACCAAAGGACGAAACACCGAACCAATACCAACTGCT533930.4637225456980751No Hit
TAAGTAAACTCTTCACGAAAGGACGAAACACCGAACCAATACCAACTGCT526610.45736506618857026No Hit
TAAGTACACTCTTCACCAAAGGACGAAACACCGAACCACTACCAACTGCT509060.44212274851209366No Hit
TAAGTAGACTCTTCACGAAAGGACGAAACACCTAACCACTACCAACTGCT486270.4223294482359167No Hit
TAAGTAAACTCTTCACGAAAGGACGAAACACCGAACCACTACCAACTGCT477160.4144173391742242No Hit
TAAGTAGACTCTTCACGAAAGGACGAAACACCGAACCCCTACCAACTGCT473990.41166416840303155No Hit
TAAGTAGACTCTTCACGAAAGGACGAAACACCTAACCAATACCAACTGCT471890.4098403013306326No Hit
TAAGTAGACTCTTCTGCAAAGGACGAAACACCGAACCACTACCAACTGCT455620.39570967406018953No Hit
TAAGTAAACTCTTCACCAAAGGACGAAACACCGAACCAATACCAACTGCT416430.3616728404567067No Hit
TAAGTAGAGTCTTCAGCAAAGGACGAAACACCGAACCACTACCAACTGCT406190.3527793172084377No Hit
TAAGTAGACTCTTCTGCAAAGGACGAAACACCGAACCAATACCAACTGCT402020.34915763830753127No Hit
TAAGTAGACTCTTCACGAAAGGACGAAACACCGAACCCATACCAACTGCT394930.3429999156678606No Hit
TAAGTACAGTCTTCACGAAAGGACGAAACACCGAACCACTACCAACTGCT374610.3253518304720767No Hit
TAAGTAGAGTCTTCAGCAAAGGACGAAACACCGAACCAATACCAACTGCT370320.3216259305956046No Hit
TAAGTAAACTCTTCACCAAAGGACGAAACACCGAACCACTACCAACTGCT354760.30811194409725834No Hit
TAAGTATACTCTTCACGAAAGGACGAAACACCGAACCAATACCAACTGCT347960.30220608881520467No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCACTACCAACTGCT344260.2989926087352637No Hit
TAAGTACAGTCTTCACGAAAGGACGAAACACCGAACCAATACCAACTGCT343680.2984888740200297No Hit
TAAGTATACTCTTCACGAAAGGACGAAACACCGAACCACTACCAACTGCT335920.29174925093345083No Hit
TAAGTAGACTCTTCACCAAAGGACGAAACACCTAACCAATACCAACTGCT335270.29118472064913686No Hit
TAAGTACACTCTTCAGGAAAGGACGAAACACCGAACCACTACCAACTGCT323120.28063234687311456No Hit
TAAGTAGACTCTTCAGGAAAGGACGAAACACCGAACCCCTACCAACTGCT322720.280284943621229No Hit
TAAGTACACTCTTCAGGAAAGGACGAAACACCGAACCAATACCAACTGCT321810.2794946012231895No Hit
TAAGTACACTCTTCTCGAAAGGACGAAACACCGAACCACTACCAACTGCT314030.27273760797401636No Hit
TAAGTACACTCTTCTCGAAAGGACGAAACACCGAACCAATACCAACTGCT313680.2724336301286165No Hit
TAAGTAGACTCTTCTCGAAAGGACGAAACACCGAACCCCTACCAACTGCT304350.264330449278387No Hit
TAAGTAGACTCTTCACGAAAGGACGAAACACCGAACCACTACCAACTGGT298690.259414693264207No Hit
TAAGTAGACTCTTCACCAAAGGACGAAACACCTAACCACTACCAACTGCT288510.2505732805037208No Hit
TAAGTAGAGTCTTCTCCAAAGGACGAAACACCGAACCACTACCAACTGCT272060.23628632176992925No Hit
TAAGTAGACTCTTCAGGAAAGGACGAAACACCTAACCACTACCAACTGCT270170.2346448414047702No Hit
TAAGTAGAGTCTTCTGGAAAGGACGAAACACCGAACCACTACCAACTGGT268500.23319443282814822No Hit
TAAGTAGACTCTTCACGAAAGGACGAAACACCGAACCAATACCAACTGGT263800.22911244461849348No Hit
TAAGTAGACTCTTCTCGAAAGGACGAAACACCTAACCACTACCAACTGCT259550.22542128506720993No Hit
TAATTAAACTCTTCACCAAAGGACGAAACACCGAACCAATACCAACTGCT255580.22197330779224625No Hit
TAAGTAGACTCTTCAGGAAAGGACGAAACACCTAACCAATACCAACTGCT251570.21849059019209402No Hit
TAAGTAGAGTCTTGAGGAAAGGACGAAACACCGAACCACTACCAACTGCT246020.2136703700721826No Hit
TAAGTACAGTCTTCAGGAAAGGACGAAACACCGAACCACTACCAACTGCT244730.21254999458485183No Hit
TAAGTAGACTCTTCTGGAAAGGACGAAACACCGAACCCCTACCAACTGCT243850.21178570743070368No Hit
TAAGTAGAGTCTTCTCCAAAGGACGAAACACCGAACCAATACCAACTGCT242210.2103613540979731No Hit
TAAGTAGAGTCTTCTGCAAAGGACGAAACACCGAACCACTACCAACTGCT239770.2082421942614715No Hit
TAAGTAGACTCTTCAGGAAAGGACGAAACACCGAACCCATACCAACTGCT238010.20671361995317525No Hit
TAAGTACAGTCTTCTCGAAAGGACGAAACACCGAACCACTACCAACTGCT235400.2044468137346223No Hit
TAAGTAGACTCTTCTCGAAAGGACGAAACACCTAACCAATACCAACTGCT234830.20395176410068547No Hit
TAAGTATACTCTTCACCAAAGGACGAAACACCGAACCAATACCAACTGCT233020.2023797643859035No Hit
TAAGTAGAGTCTTCAGGAAAGGACGAAACACCGAACCACTACCAACTGGT230210.1999392565414078No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCAATACCAACTGCT229310.19915759922466544No Hit
TAAGTAGACTCTTCTCGAAAGGACGAAACACCGAACCCATACCAACTGCT228590.1985322733712715No Hit
TAAGTAGAGTCTTCACGAAAGGACGAAACACCGAACCACTACCAACTGGT222340.19310409756056043No Hit
TAATTAAACTCTTCACGAAAGGACGAAACACCGAACCAATACCAACTGCT219270.19043777760233915No Hit
TAAGTACACTCTTCAGCAAAGGACGAAACACCGAACCAATACCAACTGCT216840.18832730284713467No Hit
TAAGTAGACTCTTCACCAAAGGACGAAACACCGAACCCCTACCAACTGCT214480.18627762366101017No Hit
TAAGTACAGTCTTCAGGAAAGGACGAAACACCGAACCAATACCAACTGCT213710.18560887240113055No Hit
TAAGTAGACTCTTCACCAAAGGACGAAACACCGAACCCATACCAACTGCT212700.18473167919011965No Hit
TAAGTAAAGTCTTCACGAAAGGACGAAACACCGAACCAATACCAACTGCT212530.1845840328080683No Hit
TAAGTATACTCTTCACCAAAGGACGAAACACCGAACCACTACCAACTGCT212240.1843321654504513No Hit
TAAGTAAAGTCTTCACGAAAGGACGAAACACCGAACCACTACCAACTGCT209040.18155293943536724No Hit
TAAGTAGAGTCTTCTCGAAAGGACGAAACACCGAACCACTACCAACTGGT208390.18098840915105327No Hit
TAAGTACAGTCTTCTCGAAAGGACGAAACACCGAACCAATACCAACTGCT207190.17994619939539674No Hit
TAAGTACACTCTTCAGCAAAGGACGAAACACCGAACCACTACCAACTGCT203970.17714960321771842No Hit
TAAGTAGACTCTTCAGGAAAGGACGAAACACCGAACCACTACCAACTGGT201770.1752388853323481No Hit
TAAGTAGAGTCTTCTGCAAAGGACGAAACACCGAACCAATACCAACTGCT199960.17366688561756616No Hit
TAAGTAGAGTCTTGTCGAAAGGACGAAACACCGAACCACTACCAACTGCT197410.17145218988679603No Hit
TAAGTATAGTCTTCACGAAAGGACGAAACACCGAACCACTACCAACTGCT193700.16823002472555793No Hit
TAAGTACACTCTTCACGAAAGGACGAAACACCGAACCACTACCAACTGGT186180.16169884359011036No Hit
TAAGTATAGTCTTCACGAAAGGACGAAACACCGAACCAATACCAACTGCT185960.16150777180157333No Hit
TAAGTAAACTCTTCAGGAAAGGACGAAACACCGAACCAATACCAACTGCT184810.1605089874524025No Hit
TAAGTAGACTCTTCTCGAAAGGACGAAACACCGAACCACTACCAACTGGT183250.159154114770049No Hit
TAAGTACAGTCTTCTGGAAAGGACGAAACACCGAACCACTACCAACTGCT183060.1589890982254034No Hit
TAATTAAACTCTTCACCAAAGGACGAAACACCGAACCACTACCAACTGCT182280.15831166188422666No Hit
TAAGTAGACTCTTGACGAAAGGACGAAACACCGAACCACTACCAACTGCT182270.1583029768029295No Hit
TAAGTAGAGTCTTGACGAAAGGACGAAACACCGAACCACTACCAACTGCT180790.15701758477095312No Hit
TAAGTAGAGTCTTCACGAAAGGACGAAACACCGAACCAATACCAACTGGT179530.1559232645275138No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCACTACCAACTGGT179190.1556279717634111No Hit
TAAGTAGAGTCTTGAGGAAAGGACGAAACACCGAACCAATACCAACTGCT178930.1554021596496855No Hit
TAAGTAAACTCTTCAGGAAAGGACGAAACACCGAACCACTACCAACTGCT174800.1518152210739676No Hit
TAAGTAAACTCTTCTCGAAAGGACGAAACACCGAACCAATACCAACTGCT174740.1517631105861848No Hit
TAATTAAACTCTTCACGAAAGGACGAAACACCGAACCACTACCAACTGCT174700.15172837026099625No Hit
TAAGTACAGTCTTCACCAAAGGACGAAACACCGAACCAATACCAACTGCT174580.1516241492854306No Hit
TAAGTAGAGTCTTCTGGAAAGGACGAAACACCGAACCAATACCAACTGGT173490.15067747542404258No Hit
TAAGTACACTCTTCACGAAAGGACGAAACACCGAACCAATACCAACTGGT173280.1504950887168027No Hit
TAAGTACAGTCTTCACCAAAGGACGAAACACCGAACCACTACCAACTGCT171590.1490273099775864No Hit
TAAGTAAACTCTTCTCGAAAGGACGAAACACCGAACCACTACCAACTGCT169330.1470644816044333No Hit
TAAGTAGAGTCTTCAGGAAAGGACGAAACACCGAACCAATACCAACTGGT167130.145153763719063No Hit
TAAGTAGACTCTTCTGGAAAGGACGAAACACCGAACCCATACCAACTGCT167030.14506691290609158No Hit
TAAGTAGACTCTTCAGCAAAGGACGAAACACCTAACCAATACCAACTGCT166260.144398161646212No Hit
TAAGTAGACTCTTCTGGAAAGGACGAAACACCTAACCACTACCAACTGCT163710.1421834659154419No Hit
TAAGTAGACTCTTCAGGAAAGGACGAAACACCGAACCAATACCAACTGGT160270.13919579794922649No Hit
TAAGTAGACTCTTGACGAAAGGACGAAACACCGAACCAATACCAACTGCT159470.13850099144545547No Hit
TAAGTACACTCTTCTGGAAAGGACGAAACACCGAACCACTACCAACTGCT159470.13850099144545547No Hit
TAAGTAGACTCTTGAGGAAAGGACGAAACACCGAACCACTACCAACTGCT159220.13828386441302704No Hit
TAAGTAGAGTCTTCTGGAAAGGACGAAACACCGAACCCCTACCAACTGCT158410.13758037282795887No Hit
TAAGTATACTCTTCAGGAAAGGACGAAACACCGAACCACTACCAACTGCT153210.13306413055344726No Hit
TAAGTAGAGTCTTCTCGAAAGGACGAAACACCGAACCAATACCAACTGGT152310.13228247323670486No Hit
TAAGTATACTCTTCAGGAAAGGACGAAACACCGAACCAATACCAACTGCT152270.1322477329115163No Hit
TAAGTAGACTCTTCAGCAAAGGACGAAACACCTAACCACTACCAACTGCT151400.13149213083866532No Hit
TAAGTAGACTCTTCTGGAAAGGACGAAACACCGAACCACTACCAACTGGT150410.13063230779024868No Hit
TAAGTACAGTCTTCTGGAAAGGACGAAACACCGAACCAATACCAACTGCT149190.12957272787199786No Hit
TAAGTAGAGTCTTGTCGAAAGGACGAAACACCGAACCAATACCAACTGCT148920.12933823067697517No Hit
TAAGTAGACTCTTCTCGAAAGGACGAAACACCGAACCAATACCAACTGGT148770.1292079544575181No Hit
TAAGTACACTCTTCTCCAAAGGACGAAACACCGAACCAATACCAACTGCT148440.12892134677471256No Hit
TAAGTAGAGTCTTGACGAAAGGACGAAACACCGAACCAATACCAACTGCT148040.12857394352282706No Hit
TAAGTACACTCTTCTGGAAAGGACGAAACACCGAACCAATACCAACTGCT147350.12797467291332454No Hit
TAAGTATACTCTTCTCGAAAGGACGAAACACCGAACCACTACCAACTGCT146490.12722775592177069No Hit
TAAGTATACTCTTCTCGAAAGGACGAAACACCGAACCAATACCAACTGCT143850.12493489445932633No Hit
TAAGTAGAGTCTTCACGAAAGGACGAAACACCGAACCCCTACCAACTGCT142990.12418797746777249No Hit
TAAGTAAACTCTTCACGAAAGGACGAAACACCGAACCAATACCAACTGGT140960.12242490596445353No Hit
TAAGTACACTCTTCTCCAAAGGACGAAACACCGAACCACTACCAACTGCT139500.12115688409507142No Hit
TAAGTAGACTCTTCTGGAAAGGACGAAACACCTAACCAATACCAACTGCT139260.12094844214394013No Hit
TAATTACACTCTTCACCAAAGGACGAAACACCGAACCAATACCAACTGCT139060.12077474051799734No Hit
TAATTACACTCTTCACGAAAGGACGAAACACCGAACCAATACCAACTGCT138510.12029706104665477No Hit
TAAGTAAACTCTTCACGAAAGGACGAAACACCGAACCACTACCAACTGGT138440.12023626547757482No Hit
TAAGTAGACTCTTGTGGAAAGGACGAAACACCGAACCACTACCAACTGCT138320.12013204450200916No Hit
TAAGTAGAGTCTTCAGGAAAGGACGAAACACCGAACCCCTACCAACTGCT137150.11911588999024404No Hit
TAAGTAAACTCTTCAGCAAAGGACGAAACACCGAACCAATACCAACTGCT133780.11618901759310864No Hit
TAAGTATAGTCTTCAGGAAAGGACGAAACACCGAACCACTACCAACTGCT132070.11470386869129809No Hit
TAAGTAGACTCTTGAGGAAAGGACGAAACACCGAACCAATACCAACTGCT130870.11366165893564155No Hit
TAAGTATAGTCTTCTCGAAAGGACGAAACACCGAACCACTACCAACTGCT129990.11289737178149344No Hit
TAAGTAGAGTCTTCTCGAAAGGACGAAACACCGAACCCCTACCAACTGCT129880.11280183588722491No Hit
TAAGTAGACTCTTCAGCAAAGGACGAAACACCGAACCCCTACCAACTGCT127780.110977968814826No Hit
TAAGTAGACTCTTCACCAAAGGACGAAACACCGAACCACTACCAACTGGT125550.10904119568556428No Hit
TAAGTAGACTCTTCAGCAAAGGACGAAACACCGAACCCATACCAACTGCT121610.105619273654492No Hit
TAAGTAGACTCTTGTCGAAAGGACGAAACACCGAACCACTACCAACTGCT120490.10464654454921257No Hit
TAAGTAGACTCTTCACCAAAGGACGAAACACCGAACCAATACCAACTGGT120140.10434256670381276No Hit
TAAGTAAACTCTTCAGCAAAGGACGAAACACCGAACCACTACCAACTGCT119050.10339589284242474No Hit
TAAGTATAGTCTTCAGGAAAGGACGAAACACCGAACCAATACCAACTGCT118310.10275319682643655No Hit
TAAGTAAAGTCTTCACCAAAGGACGAAACACCGAACCAATACCAACTGCT118090.10256212503789953No Hit
TAAGTAGAGTCTTCACGAAAGGACGAAACACCTAACCAATACCAACTGCT117890.10238842341195678No Hit
TAAGTAGAGTCTTCACGAAAGGACGAAACACCTAACCACTACCAACTGCT116190.10091195959144336No Hit
TAAGTACACTCTTCACGAAAGGACGAAACACCTAACCAATACCAACTGCT116170.10089458942884907No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)