FastQCFastQC Report
Mon 21 Nov 2022
H3TJ5BGXN_n01_R14_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3TJ5BGXN_n01_R14_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15717511
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGTAGACTCTTCACGAAAGGACGAAACACCGAACCACTACCAACTGCT5520463.5122991165713198No Hit
TAAGTAGACTCTTCACGAAAGGACGAAACACCGAACCAATACCAACTGCT5062543.2209552772064227No Hit
TAAGTAGACTCTTCAGGAAAGGACGAAACACCGAACCACTACCAACTGCT3163532.012742348327289No Hit
TAAGTAGACTCTTCACCAAAGGACGAAACACCGAACCAATACCAACTGCT3121481.985988748472961No Hit
TAAGTAGACTCTTCACCAAAGGACGAAACACCGAACCACTACCAACTGCT2992371.9038446990748088No Hit
TAAGTAGACTCTTCTCGAAAGGACGAAACACCGAACCACTACCAACTGCT2833561.8028045280197353No Hit
TAAGTAGACTCTTCAGGAAAGGACGAAACACCGAACCAATACCAACTGCT2724081.7331497334406192No Hit
TAAGTAGACTCTTCTCGAAAGGACGAAACACCGAACCAATACCAACTGCT2449131.5582174556773016No Hit
TAAGTAGACTCTTCTGGAAAGGACGAAACACCGAACCACTACCAACTGCT1888471.20150703250661No Hit
TAAGTAGACTCTTCAGCAAAGGACGAAACACCGAACCAATACCAACTGCT1576481.0030086824815965No Hit
TAAGTAGACTCTTCAGCAAAGGACGAAACACCGAACCACTACCAACTGCT1572201.000285605017232No Hit
TAAGTAGAGTCTTCACGAAAGGACGAAACACCGAACCACTACCAACTGCT1566590.9967163375931469No Hit
TAAGTAGACTCTTCTGGAAAGGACGAAACACCGAACCAATACCAACTGCT1509260.9602410966978168No Hit
TAAGTAGAGTCTTCACGAAAGGACGAAACACCGAACCAATACCAACTGCT1362530.8668866209159961No Hit
TAAGTAGAGTCTTCAGGAAAGGACGAAACACCGAACCACTACCAACTGCT1249560.7950113729839287No Hit
TAAGTACACTCTTCACGAAAGGACGAAACACCGAACCAATACCAACTGCT1232360.7840681644822772No Hit
TAAGTACACTCTTCACGAAAGGACGAAACACCGAACCACTACCAACTGCT1155720.7353072633446861No Hit
TAAGTAGAGTCTTCTCGAAAGGACGAAACACCGAACCACTACCAACTGCT1122330.714063441724329No Hit
TAAGTAGAGTCTTCTGGAAAGGACGAAACACCGAACCACTACCAACTGCT1097680.6983802969821367No Hit
TAAGTAGAGTCTTCAGGAAAGGACGAAACACCGAACCAATACCAACTGCT1009450.6422454547669794No Hit
TAAGTACACTCTTCACCAAAGGACGAAACACCGAACCAATACCAACTGCT989800.629743475286895No Hit
TAAGTAGACTCTTCTCCAAAGGACGAAACACCGAACCACTACCAACTGCT962250.61221525469268No Hit
TAAGTAGACTCTTCTCCAAAGGACGAAACACCGAACCAATACCAACTGCT960060.6108219043078768No Hit
TAAGTAGAGTCTTCTCGAAAGGACGAAACACCGAACCAATACCAACTGCT894140.5688814214922452No Hit
TAAGTACACTCTTCACCAAAGGACGAAACACCGAACCACTACCAACTGCT868720.5527083772996882No Hit
TAAGTAAACTCTTCACGAAAGGACGAAACACCGAACCAATACCAACTGCT864870.550258880047865No Hit
TAAGTAGAGTCTTCTGGAAAGGACGAAACACCGAACCAATACCAACTGCT806750.513281015041122No Hit
TAAGTAGAGTCTTCACCAAAGGACGAAACACCGAACCAATACCAACTGCT781870.4974515366968727No Hit
TAAGTAGAGTCTTCACCAAAGGACGAAACACCGAACCACTACCAACTGCT778320.49519290936077603No Hit
TAAGTAAACTCTTCACGAAAGGACGAAACACCGAACCACTACCAACTGCT763430.48571939921021845No Hit
TAAGTAAACTCTTCACCAAAGGACGAAACACCGAACCAATACCAACTGCT744330.47356734790896593No Hit
TAAGTAGACTCTTCACGAAAGGACGAAACACCTAACCAATACCAACTGCT724240.4607854258858161No Hit
TAAGTAGACTCTTCACGAAAGGACGAAACACCTAACCACTACCAACTGCT705410.44880515750871747No Hit
TAAGTAGACTCTTCACGAAAGGACGAAACACCGAACCCCTACCAACTGCT620560.39482078301074514No Hit
TAAGTAAACTCTTCACCAAAGGACGAAACACCGAACCACTACCAACTGCT608730.3872941460005977No Hit
TAAGTAGACTCTTCTGCAAAGGACGAAACACCGAACCACTACCAACTGCT582840.3708220722734026No Hit
TAAGTAGACTCTTCACCAAAGGACGAAACACCTAACCAATACCAACTGCT560070.3563350456697628No Hit
TAAGTAGACTCTTCTGCAAAGGACGAAACACCGAACCAATACCAACTGCT543400.34572904068589483No Hit
TAAGTAGACTCTTCACGAAAGGACGAAACACCGAACCCATACCAACTGCT536100.3410845394032172No Hit
TAAGTACAGTCTTCACGAAAGGACGAAACACCGAACCACTACCAACTGCT534480.34005384185829424No Hit
TAAGTAGAGTCTTCAGCAAAGGACGAAACACCGAACCACTACCAACTGCT531570.3382024036757474No Hit
TAAGTACAGTCTTCACGAAAGGACGAAACACCGAACCAATACCAACTGCT511930.3257067865261873No Hit
TAAGTATACTCTTCACGAAAGGACGAAACACCGAACCAATACCAACTGCT510320.32468245131178847No Hit
TAAGTAGAGTCTTCAGCAAAGGACGAAACACCGAACCAATACCAACTGCT507330.3227801144850479No Hit
TAAGTACACTCTTCAGGAAAGGACGAAACACCGAACCAATACCAACTGCT501300.3189436291789457No Hit
TAAGTACACTCTTCAGGAAAGGACGAAACACCGAACCACTACCAACTGCT489530.3114551661519435No Hit
TAAGTATACTCTTCACGAAAGGACGAAACACCGAACCACTACCAACTGCT472520.3006328419302522No Hit
TAAGTACACTCTTCTCGAAAGGACGAAACACCGAACCAATACCAACTGCT466860.29703176285354593No Hit
TAAGTAGACTCTTCACCAAAGGACGAAACACCTAACCACTACCAACTGCT466010.2964909647589876No Hit
TAAGTACACTCTTCTCGAAAGGACGAAACACCGAACCACTACCAACTGCT456960.2907330556345722No Hit
TAATTAAACTCTTCACCAAAGGACGAAACACCGAACCAATACCAACTGCT410220.2609955227643868No Hit
TAAGTAGACTCTTCAGGAAAGGACGAAACACCGAACCCCTACCAACTGCT396950.25255271015875225No Hit
TAAGTAGACTCTTCACGAAAGGACGAAACACCGAACCACTACCAACTGGT386130.2456686685315506No Hit
TAAGTATACTCTTCACCAAAGGACGAAACACCGAACCAATACCAACTGCT383240.24382995501005217No Hit
TAAGTAGACTCTTCAGGAAAGGACGAAACACCTAACCACTACCAACTGCT380500.24208667644641696No Hit
TAAGTACACTCTTCAGCAAAGGACGAAACACCGAACCAATACCAACTGCT368990.23476363401304443No Hit
TAAGTAGACTCTTCAGGAAAGGACGAAACACCTAACCAATACCAACTGCT362840.23085080074065162No Hit
TAAGTAGACTCTTCTCGAAAGGACGAAACACCGAACCCCTACCAACTGCT356110.2265689522978543No Hit
TAAGTAGACTCTTCACGAAAGGACGAAACACCGAACCAATACCAACTGGT354530.22556370407502815No Hit
TAAGTAGAGTCTTCTCCAAAGGACGAAACACCGAACCACTACCAACTGCT346850.22067743423243033No Hit
TAAGTAGACTCTTCTCGAAAGGACGAAACACCTAACCACTACCAACTGCT342180.21770622587762145No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCACTACCAACTGCT342050.21762351558080664No Hit
TAAGTACACTCTTCAGCAAAGGACGAAACACCGAACCACTACCAACTGCT335600.21351981239268736No Hit
TAATTAAACTCTTCACGAAAGGACGAAACACCGAACCAATACCAACTGCT335600.21351981239268736No Hit
TAAGTACAGTCTTCAGGAAAGGACGAAACACCGAACCACTACCAACTGCT335020.21315079722228283No Hit
TAAGTAGACTCTTCTCGAAAGGACGAAACACCTAACCAATACCAACTGCT327120.20812455610815223No Hit
TAAGTATACTCTTCACCAAAGGACGAAACACCGAACCACTACCAACTGCT326990.20804184581133742No Hit
TAAGTAAAGTCTTCACGAAAGGACGAAACACCGAACCAATACCAACTGCT321880.20479069491346308No Hit
TAAGTAGAGTCTTCTCCAAAGGACGAAACACCGAACCAATACCAACTGCT320040.20362002609700733No Hit
TAAGTAGACTCTTCACCAAAGGACGAAACACCGAACCCATACCAACTGCT318780.20281837245095616No Hit
TAAGTAGACTCTTCAGGAAAGGACGAAACACCGAACCCATACCAACTGCT311310.19806571154936684No Hit
TAAGTACAGTCTTCAGGAAAGGACGAAACACCGAACCAATACCAACTGCT309460.19688868040238688No Hit
TAAGTACAGTCTTCTCGAAAGGACGAAACACCGAACCACTACCAACTGCT308590.19633515764678008No Hit
TAAGTAAAGTCTTCACGAAAGGACGAAACACCGAACCACTACCAACTGCT306130.19477002433782295No Hit
TAAGTAGACTCTTCACCAAAGGACGAAACACCGAACCCCTACCAACTGCT300050.19090172737909966No Hit
TAAGTAAACTCTTCAGGAAAGGACGAAACACCGAACCAATACCAACTGCT294840.18758695317598315No Hit
TAATTAAACTCTTCACCAAAGGACGAAACACCGAACCACTACCAACTGCT288480.1835405109625818No Hit
TAAGTAGAGTCTTCTGCAAAGGACGAAACACCGAACCACTACCAACTGCT288390.18348324998786386No Hit
TAAGTACAGTCTTCACCAAAGGACGAAACACCGAACCAATACCAACTGCT285260.18149184053378428No Hit
TAAGTAGAGTCTTCTGGAAAGGACGAAACACCGAACCACTACCAACTGGT282940.18001577985216616No Hit
TAAGTACAGTCTTCTCGAAAGGACGAAACACCGAACCAATACCAACTGCT281290.17896599531567053No Hit
TAAGTAGACTCTTCTCGAAAGGACGAAACACCGAACCCATACCAACTGCT280930.17873695141679874No Hit
TAAGTAGACTCTTCTGGAAAGGACGAAACACCGAACCCCTACCAACTGCT276130.1756830327651751No Hit
TAAGTACACTCTTCACGAAAGGACGAAACACCGAACCACTACCAACTGGT273560.1740479138204516No Hit
TAAGTACACTCTTCACGAAAGGACGAAACACCGAACCAATACCAACTGGT270440.17206286669689622No Hit
TAAGTAAACTCTTCTCGAAAGGACGAAACACCGAACCAATACCAACTGCT268450.17079676292257726No Hit
TAAGTAGAGTCTTGAGGAAAGGACGAAACACCGAACCACTACCAACTGCT267960.17048500872689068No Hit
TAAGTACAGTCTTCACCAAAGGACGAAACACCGAACCACTACCAACTGCT264170.1680736854582128No Hit
TAAGTAGAGTCTTCACGAAAGGACGAAACACCGAACCACTACCAACTGGT264130.16804823613611597No Hit
TAAGTAAACTCTTCAGGAAAGGACGAAACACCGAACCACTACCAACTGCT263810.16784464155934103No Hit
TAAGTAGACTCTTCAGCAAAGGACGAAACACCTAACCAATACCAACTGCT263640.16773648194042937No Hit
TAAGTAGAGTCTTCAGGAAAGGACGAAACACCGAACCACTACCAACTGGT258730.16461257765303933No Hit
TAATTAAACTCTTCACGAAAGGACGAAACACCGAACCACTACCAACTGCT258720.16460621532251513No Hit
TAAGTAGAGTCTTCTGCAAAGGACGAAACACCGAACCAATACCAACTGCT250240.15921095903798No Hit
TAAGTAGACTCTTCAGGAAAGGACGAAACACCGAACCACTACCAACTGGT248760.15826933412039604No Hit
TAAGTATAGTCTTCACGAAAGGACGAAACACCGAACCACTACCAACTGCT248560.1581420875099117No Hit
TAAGTAAACTCTTCTCGAAAGGACGAAACACCGAACCACTACCAACTGCT243040.15463008106054452No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCAATACCAACTGCT242570.15433105152590637No Hit
TAAGTATAGTCTTCACGAAAGGACGAAACACCGAACCAATACCAACTGCT240330.152905889488482No Hit
TAAGTACACTCTTCTCCAAAGGACGAAACACCGAACCAATACCAACTGCT236580.150520015541901No Hit
TAAGTAGACTCTTCAGCAAAGGACGAAACACCTAACCACTACCAACTGCT233370.14847770744362768No Hit
TAAGTAAACTCTTCAGCAAAGGACGAAACACCGAACCAATACCAACTGCT232860.14815322858689267No Hit
TAAGTAGACTCTTGACGAAAGGACGAAACACCGAACCACTACCAACTGCT228600.14544287578357668No Hit
TAATTACACTCTTCACCAAAGGACGAAACACCGAACCAATACCAACTGCT227790.1449275270111152No Hit
TAAGTAGAGTCTTCTCGAAAGGACGAAACACCGAACCACTACCAACTGGT224260.14268162433606696No Hit
TAAGTACAGTCTTCTGGAAAGGACGAAACACCGAACCACTACCAACTGCT224080.14256710238663106No Hit
TAAGTAGAGTCTTCACGAAAGGACGAAACACCGAACCAATACCAACTGGT223710.1423316961572351No Hit
TAAGTACACTCTTCTGGAAAGGACGAAACACCGAACCACTACCAACTGCT222310.14144096988384483No Hit
TAAGTAAACTCTTCACGAAAGGACGAAACACCGAACCAATACCAACTGGT221950.14121192598497306No Hit
TAAGTATACTCTTCAGGAAAGGACGAAACACCGAACCAATACCAACTGCT215770.1372800057210076No Hit
TAAGTAGACTCTTCTCGAAAGGACGAAACACCGAACCACTACCAACTGGT214190.1362747574981815No Hit
TAAGTACACTCTTCTCCAAAGGACGAAACACCGAACCACTACCAACTGCT213640.13592482931934963No Hit
TAATTACACTCTTCACGAAAGGACGAAACACCGAACCAATACCAACTGCT213560.13587393067515588No Hit
TAAGTAAACTCTTCACGAAAGGACGAAACACCGAACCACTACCAACTGGT212580.13525042228378273No Hit
TAAGTACACTCTTCTGGAAAGGACGAAACACCGAACCAATACCAACTGCT211780.13474143584184542No Hit
TAAGTAGACTCTTCAGGAAAGGACGAAACACCGAACCAATACCAACTGGT210600.13399068083998797No Hit
TAAGTACACTCTTCACCAAAGGACGAAACACCTAACCAATACCAACTGCT209000.1329727079561134No Hit
TAAGTAGAGTCTTGAGGAAAGGACGAAACACCGAACCAATACCAACTGCT206570.13142666163872893No Hit
TAAGTATACTCTTCAGGAAAGGACGAAACACCGAACCACTACCAACTGCT206170.13117216841776028No Hit
TAAGTAGAGTCTTGACGAAAGGACGAAACACCGAACCACTACCAACTGCT205650.13084132723050107No Hit
TAAGTAGACTCTTGACGAAAGGACGAAACACCGAACCAATACCAACTGCT205020.13044050040747546No Hit
TAAGTAGAGTCTTGTCGAAAGGACGAAACACCGAACCACTACCAACTGCT204520.13012238388126465No Hit
TAAGTAGACTCTTCTGGAAAGGACGAAACACCTAACCACTACCAACTGCT203360.1293843535404556No Hit
TAAGTACACTCTTCACGAAAGGACGAAACACCTAACCAATACCAACTGCT199680.12704301590754413No Hit
TAAGTATACTCTTCTCGAAAGGACGAAACACCGAACCAATACCAACTGCT196900.12527428802181212No Hit
TAAGTAAACTCTTCAGCAAAGGACGAAACACCGAACCACTACCAACTGCT194860.12397637259487204No Hit
TAAGTAGAGTCTTCAGGAAAGGACGAAACACCGAACCAATACCAACTGGT194010.1234355745003137No Hit
TAAGTAAAGTCTTCACCAAAGGACGAAACACCGAACCAATACCAACTGCT193000.12279297911736789No Hit
TAAGTAGACTCTTCTGGAAAGGACGAAACACCGAACCCATACCAACTGCT192640.12256393521849611No Hit
TAAGTACAGTCTTCTGGAAAGGACGAAACACCGAACCAATACCAACTGCT191510.12184499186925969No Hit
TAAGTAGACTCTTGAGGAAAGGACGAAACACCGAACCACTACCAACTGCT189950.12085246830748202No Hit
TAAGTATACTCTTCTCGAAAGGACGAAACACCGAACCACTACCAACTGCT187370.1192109870322343No Hit
TAAGTAGAGTCTTCTGGAAAGGACGAAACACCGAACCAATACCAACTGGT185990.1183329854198925No Hit
TAAGTAGACTCTTCTGGAAAGGACGAAACACCTAACCAATACCAACTGCT184280.11724502690025158No Hit
TAAGTAGACTCTTCTCGAAAGGACGAAACACCGAACCAATACCAACTGGT182390.11604254643117476No Hit
TAAGTAGACTCTTCACCAAAGGACGAAACACCGAACCAATACCAACTGGT178990.11387935405294132No Hit
TAAGTAGACTCTTCACCAAAGGACGAAACACCGAACCACTACCAACTGGT178820.11377119443402968No Hit
TAAGTAGAGTCTTGACGAAAGGACGAAACACCGAACCAATACCAACTGCT177180.11272777222805824No Hit
TAATTACACTCTTCACGAAAGGACGAAACACCGAACCACTACCAACTGCT174170.11081271074026924No Hit
TAAGTAGACTCTTCAGCAAAGGACGAAACACCGAACCCCTACCAACTGCT172210.10956569395752293No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCACTACCAACTGGT171630.10919667878711839No Hit
TAAGTAGAGTCTTCACGAAAGGACGAAACACCGAACCCCTACCAACTGCT171120.10887219993038338No Hit
TAAGTAGAGTCTTCACGAAAGGACGAAACACCTAACCAATACCAACTGCT171070.10884038827776231No Hit
TAAGTAGACTCTTCAGCAAAGGACGAAACACCGAACCCATACCAACTGCT170650.10857317039574524No Hit
TAAGTAGAGTCTTCTCGAAAGGACGAAACACCGAACCAATACCAACTGGT170350.10838230048001873No Hit
TAATTACACTCTTCACCAAAGGACGAAACACCGAACCACTACCAACTGCT169090.10758064683396754No Hit
TAAGTAGACTCTTCTGGAAAGGACGAAACACCGAACCACTACCAACTGGT167500.1065690362806172No Hit
TAAGTAGAGTCTTCTGGAAAGGACGAAACACCGAACCCCTACCAACTGCT166780.10611094848287365No Hit
TAAGTAGACTCTTGAGGAAAGGACGAAACACCGAACCAATACCAACTGCT166540.10595825255029247No Hit
TAAGTAGACTCTTCTCCAAAGGACGAAACACCTAACCAATACCAACTGCT165720.10543654144730677No Hit
TAAGTATAGTCTTCAGGAAAGGACGAAACACCGAACCACTACCAACTGCT162590.10344513199322716No Hit
TAAGTAAAGTCTTCACCAAAGGACGAAACACCGAACCACTACCAACTGCT162440.10334969703536392No Hit
TAAGTAAAGTCTTCAGGAAAGGACGAAACACCGAACCAATACCAACTGCT161000.10243352143987684No Hit
TAAGTACACTCTTCACCAAAGGACGAAACACCGAACCAATACCAACTGGT160860.1023444488125378No Hit
TAAGTAAAGTCTTCAGGAAAGGACGAAACACCGAACCACTACCAACTGCT160330.10200724529475437No Hit
TAAGTAAACTCTTCACCAAAGGACGAAACACCTAACCAATACCAACTGCT159360.1013900992339054No Hit
TAAGTAGAGTCTTCACGAAAGGACGAAACACCTAACCACTACCAACTGCT158420.10079204016462912No Hit
TAAGTAGAGTCTTCAGGAAAGGACGAAACACCGAACCCCTACCAACTGCT158060.10056299626575735No Hit
TAAGTAGAGTCTTGTCGAAAGGACGAAACACCGAACCAATACCAACTGCT158030.10054390927418469No Hit
TAAGTACACTCTTCACGAAAGGACGAAACACCTAACCACTACCAACTGCT157580.10025760440059497No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)