FastQCFastQC Report
Mon 21 Nov 2022
H3TJ5BGXN_n01_R13_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3TJ5BGXN_n01_R13_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16219444
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGTAGACTCTTCACGAAAGGACGAAACACCGAACCACTACCAACTGCT5725233.5298558939504954No Hit
TAAGTAGACTCTTCACGAAAGGACGAAACACCGAACCAATACCAACTGCT5088583.1373331909527846No Hit
TAAGTAGACTCTTCAGGAAAGGACGAAACACCGAACCACTACCAACTGCT3423892.110978650069632No Hit
TAAGTAGACTCTTCTCGAAAGGACGAAACACCGAACCACTACCAACTGCT3144061.9384511577585521No Hit
TAAGTAGACTCTTCACCAAAGGACGAAACACCGAACCAATACCAACTGCT2970241.8312834891257679No Hit
TAAGTAGACTCTTCACCAAAGGACGAAACACCGAACCACTACCAACTGCT2930001.8064737607528347No Hit
TAAGTAGACTCTTCAGGAAAGGACGAAACACCGAACCAATACCAACTGCT2878571.7747649056280845No Hit
TAAGTAGACTCTTCTCGAAAGGACGAAACACCGAACCAATACCAACTGCT2625771.618902596167908No Hit
TAAGTAGACTCTTCTGGAAAGGACGAAACACCGAACCACTACCAACTGCT2176011.3416057911726196No Hit
TAAGTAGAGTCTTCACGAAAGGACGAAACACCGAACCACTACCAACTGCT1711351.0551224813871547No Hit
TAAGTAGACTCTTCTGGAAAGGACGAAACACCGAACCAATACCAACTGCT1681781.036891276914301No Hit
TAAGTAGACTCTTCAGCAAAGGACGAAACACCGAACCACTACCAACTGCT1612370.9940969616467741No Hit
TAAGTAGACTCTTCAGCAAAGGACGAAACACCGAACCAATACCAACTGCT1561700.9628566799207174No Hit
TAAGTAGAGTCTTCACGAAAGGACGAAACACCGAACCAATACCAACTGCT1445980.8915102145301652No Hit
TAAGTAGAGTCTTCAGGAAAGGACGAAACACCGAACCACTACCAACTGCT1433990.8841178526218284No Hit
TAAGTAGAGTCTTCTGGAAAGGACGAAACACCGAACCACTACCAACTGCT1323950.8162733568425651No Hit
TAAGTAGAGTCTTCTCGAAAGGACGAAACACCGAACCACTACCAACTGCT1302030.8027587135539295No Hit
TAAGTACACTCTTCACGAAAGGACGAAACACCGAACCAATACCAACTGCT1164170.7177619652066989No Hit
TAAGTACACTCTTCACGAAAGGACGAAACACCGAACCACTACCAACTGCT1129860.6966083424314669No Hit
TAAGTAGAGTCTTCAGGAAAGGACGAAACACCGAACCAATACCAACTGCT1116040.6880877051025917No Hit
TAAGTAGAGTCTTCTCGAAAGGACGAAACACCGAACCAATACCAACTGCT1026940.6331536395452273No Hit
TAAGTAGACTCTTCTCCAAAGGACGAAACACCGAACCACTACCAACTGCT994850.6133687443293371No Hit
TAAGTAGACTCTTCTCCAAAGGACGAAACACCGAACCAATACCAACTGCT973250.6000513951033093No Hit
TAAGTAGAGTCTTCTGGAAAGGACGAAACACCGAACCAATACCAACTGCT947130.5839472672429462No Hit
TAAGTACACTCTTCACCAAAGGACGAAACACCGAACCAATACCAACTGCT892160.5500558465505969No Hit
TAAGTAAACTCTTCACGAAAGGACGAAACACCGAACCAATACCAACTGCT798910.49256312361878746No Hit
TAAGTAGAGTCTTCACCAAAGGACGAAACACCGAACCACTACCAACTGCT792370.48853092621424016No Hit
TAAGTACACTCTTCACCAAAGGACGAAACACCGAACCACTACCAACTGCT790260.48723001848891984No Hit
TAAGTAGAGTCTTCACCAAAGGACGAAACACCGAACCAATACCAACTGCT787150.4853125668179501No Hit
TAAGTAAACTCTTCACGAAAGGACGAAACACCGAACCACTACCAACTGCT711730.43881282243707No Hit
TAAGTAGACTCTTCACGAAAGGACGAAACACCTAACCACTACCAACTGCT710960.4383380835989199No Hit
TAAGTAGACTCTTCACGAAAGGACGAAACACCTAACCAATACCAACTGCT704780.43452784201480643No Hit
TAAGTAAACTCTTCACCAAAGGACGAAACACCGAACCAATACCAACTGCT660670.40733208857221No Hit
TAAGTAGACTCTTCACGAAAGGACGAAACACCGAACCCCTACCAACTGCT659210.40643193441156183No Hit
TAAGTAGACTCTTCTGCAAAGGACGAAACACCGAACCACTACCAACTGCT631680.389458479587833No Hit
TAAGTAGACTCTTCTGCAAAGGACGAAACACCGAACCAATACCAACTGCT574480.3541921658966855No Hit
TAAGTAGAGTCTTCAGCAAAGGACGAAACACCGAACCACTACCAACTGCT573680.3536989307401659No Hit
TAAGTAAACTCTTCACCAAAGGACGAAACACCGAACCACTACCAACTGCT555100.3422435442299995No Hit
TAAGTAGACTCTTCACGAAAGGACGAAACACCGAACCCATACCAACTGCT555100.3422435442299995No Hit
TAAGTACAGTCTTCACGAAAGGACGAAACACCGAACCACTACCAACTGCT542510.33448125595427314No Hit
TAAGTAGAGTCTTCAGCAAAGGACGAAACACCGAACCAATACCAACTGCT527350.3251344497382278No Hit
TAAGTAGACTCTTCACCAAAGGACGAAACACCTAACCAATACCAACTGCT515730.31797020908978135No Hit
TAAGTACAGTCTTCACGAAAGGACGAAACACCGAACCAATACCAACTGCT510350.31465320266218744No Hit
TAAGTATACTCTTCACGAAAGGACGAAACACCGAACCAATACCAACTGCT508790.31369139410697433No Hit
TAAGTACACTCTTCAGGAAAGGACGAAACACCGAACCAATACCAACTGCT489010.3014961548620286No Hit
TAAGTACACTCTTCAGGAAAGGACGAAACACCGAACCACTACCAACTGCT485280.2991964459447562No Hit
TAAGTATACTCTTCACGAAAGGACGAAACACCGAACCACTACCAACTGCT477610.29446755388162504No Hit
TAAGTACACTCTTCTCGAAAGGACGAAACACCGAACCACTACCAACTGCT461440.2844980382804737No Hit
TAAGTACACTCTTCTCGAAAGGACGAAACACCGAACCAATACCAACTGCT458990.2829875056136326No Hit
TAAGTAGACTCTTCACCAAAGGACGAAACACCTAACCACTACCAACTGCT443970.27372701554997814No Hit
TAAGTAGACTCTTCAGGAAAGGACGAAACACCGAACCCCTACCAACTGCT440240.27142730663270576No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCACTACCAACTGCT429710.26493509888501726No Hit
TAAGTAGACTCTTCACGAAAGGACGAAACACCGAACCACTACCAACTGGT421570.2599164311674309No Hit
TAAGTAGACTCTTCTCGAAAGGACGAAACACCGAACCCCTACCAACTGCT399180.24611201222434015No Hit
TAAGTAGACTCTTCAGGAAAGGACGAAACACCTAACCACTACCAACTGCT392180.24179620460479412No Hit
TAAGTAGACTCTTCACGAAAGGACGAAACACCGAACCAATACCAACTGGT376150.23191300515603372No Hit
TAAGTAGAGTCTTCTCCAAAGGACGAAACACCGAACCACTACCAACTGCT373160.2300695387585419No Hit
TAATTAAACTCTTCACCAAAGGACGAAACACCGAACCAATACCAACTGCT371000.2287378038359391No Hit
TAAGTAGACTCTTCAGGAAAGGACGAAACACCTAACCAATACCAACTGCT368520.22720877485072855No Hit
TAAGTAGACTCTTCTCGAAAGGACGAAACACCTAACCACTACCAACTGCT361870.22310875761215984No Hit
TAAGTATACTCTTCACCAAAGGACGAAACACCGAACCAATACCAACTGCT354760.21872513015859235No Hit
TAAGTACAGTCTTCAGGAAAGGACGAAACACCGAACCACTACCAACTGCT352860.21755369666185842No Hit
TAAGTACACTCTTCAGCAAAGGACGAAACACCGAACCAATACCAACTGCT347590.21430451006828594No Hit
TAAGTAGAGTCTTCTGGAAAGGACGAAACACCGAACCACTACCAACTGGT344490.21239322383677267No Hit
TAAGTAGAGTCTTCTCCAAAGGACGAAACACCGAACCAATACCAACTGCT342700.2112896101740602No Hit
TAAGTAGACTCTTCAGGAAAGGACGAAACACCGAACCCATACCAACTGCT338990.20900223213570082No Hit
TAAGTAGACTCTTCTCGAAAGGACGAAACACCTAACCAATACCAACTGCT338130.20847200434244234No Hit
TAAGTACAGTCTTCTCGAAAGGACGAAACACCGAACCACTACCAACTGCT333670.2057222183448459No Hit
TAAGTAGAGTCTTCTGCAAAGGACGAAACACCGAACCACTACCAACTGCT328390.20246686631181687No Hit
TAAGTAGAGTCTTGAGGAAAGGACGAAACACCGAACCACTACCAACTGCT320070.19733722068401358No Hit
TAAGTAGACTCTTCTGGAAAGGACGAAACACCGAACCCCTACCAACTGCT319180.1967884965723856No Hit
TAAGTAAAGTCTTCACGAAAGGACGAAACACCGAACCAATACCAACTGCT317980.19604864383760628No Hit
TAAGTACACTCTTCAGCAAAGGACGAAACACCGAACCACTACCAACTGCT315410.19446412589728723No Hit
TAAGTATACTCTTCACCAAAGGACGAAACACCGAACCACTACCAACTGCT315240.19435931342652685No Hit
TAAGTACAGTCTTCAGGAAAGGACGAAACACCGAACCAATACCAACTGCT313440.19324953432435785No Hit
TAAGTAGACTCTTCACCAAAGGACGAAACACCGAACCCATACCAACTGCT311050.1917759942942557No Hit
TAATTAAACTCTTCACGAAAGGACGAAACACCGAACCAATACCAACTGCT306790.18914951708578914No Hit
TAAGTAGACTCTTCTCGAAAGGACGAAACACCGAACCCATACCAACTGCT306660.1890693663728547No Hit
TAAGTAGACTCTTCACCAAAGGACGAAACACCGAACCCCTACCAACTGCT304610.18780545128427337No Hit
TAAGTAGAGTCTTCAGGAAAGGACGAAACACCGAACCACTACCAACTGGT303940.18739236684068825No Hit
TAAGTAAAGTCTTCACGAAAGGACGAAACACCGAACCACTACCAACTGCT303620.18719507277808045No Hit
TAAGTAGAGTCTTCACGAAAGGACGAAACACCGAACCACTACCAACTGGT298360.1839520516239644No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCAATACCAACTGCT297310.18330468048103252No Hit
TAAGTACAGTCTTCTCGAAAGGACGAAACACCGAACCAATACCAACTGCT296180.18260798582244867No Hit
TAAGTAAACTCTTCAGGAAAGGACGAAACACCGAACCAATACCAACTGCT280840.1731502016961864No Hit
TAAGTAGAGTCTTCTGCAAAGGACGAAACACCGAACCAATACCAACTGCT279880.17255831950836292No Hit
TAAGTAGACTCTTCAGGAAAGGACGAAACACCGAACCACTACCAACTGGT274710.16937078730935537No Hit
TAAGTAGAGTCTTCTCGAAAGGACGAAACACCGAACCACTACCAACTGGT272810.16819935381262144No Hit
TAAGTACAGTCTTCACCAAAGGACGAAACACCGAACCAATACCAACTGCT271520.16740401212273368No Hit
TAAGTACACTCTTCACGAAAGGACGAAACACCGAACCACTACCAACTGGT271360.16730536509142976No Hit
TAAGTACACTCTTCACGAAAGGACGAAACACCGAACCAATACCAACTGGT266230.1641424946502482No Hit
TAAGTATAGTCTTCACGAAAGGACGAAACACCGAACCACTACCAACTGCT266030.1640191858611183No Hit
TAAGTAAACTCTTCTCGAAAGGACGAAACACCGAACCAATACCAACTGCT264410.1630203846691662No Hit
TAATTAAACTCTTCACCAAAGGACGAAACACCGAACCACTACCAACTGCT262430.16179962765678035No Hit
TAAGTATAGTCTTCACGAAAGGACGAAACACCGAACCAATACCAACTGCT261120.1609919550879796No Hit
TAAGTAAACTCTTCAGGAAAGGACGAAACACCGAACCACTACCAACTGCT259180.15979585983341968No Hit
TAAGTAGACTCTTCAGCAAAGGACGAAACACCTAACCAATACCAACTGCT258360.15929029379798715No Hit
TAAGTACAGTCTTCACCAAAGGACGAAACACCGAACCACTACCAACTGCT256050.15786607728353697No Hit
TAAGTAGAGTCTTGTCGAAAGGACGAAACACCGAACCACTACCAACTGCT251790.1552396000750704No Hit
TAAGTACAGTCTTCTGGAAAGGACGAAACACCGAACCACTACCAACTGCT250370.1543641076722482No Hit
TAAGTAGACTCTTGACGAAAGGACGAAACACCGAACCACTACCAACTGCT247970.15288440220268956No Hit
TAAGTAGAGTCTTCACGAAAGGACGAAACACCGAACCAATACCAACTGGT247690.15271176989790772No Hit
TAAGTAAACTCTTCTCGAAAGGACGAAACACCGAACCACTACCAACTGCT245980.1516574797508472No Hit
TAAGTAGACTCTTCTCGAAAGGACGAAACACCGAACCACTACCAACTGGT245900.15160815623519525No Hit
TAAGTAGAGTCTTGAGGAAAGGACGAAACACCGAACCAATACCAACTGCT245890.15160199079573874No Hit
TAATTAAACTCTTCACGAAAGGACGAAACACCGAACCACTACCAACTGCT242970.1498016824744424No Hit
TAAGTAGAGTCTTGACGAAAGGACGAAACACCGAACCACTACCAACTGCT240560.14831581156542728No Hit
TAAGTACACTCTTCTGGAAAGGACGAAACACCGAACCACTACCAACTGCT232710.14347594159207924No Hit
TAAGTAGACTCTTCTGGAAAGGACGAAACACCTAACCACTACCAACTGCT230380.14203939419871606No Hit
TAAGTAGAGTCTTCTGGAAAGGACGAAACACCGAACCAATACCAACTGGT226910.13989998670731252No Hit
TAAGTAGACTCTTCAGCAAAGGACGAAACACCTAACCACTACCAACTGCT226280.1395115640215534No Hit
TAAGTACACTCTTCTCCAAAGGACGAAACACCGAACCAATACCAACTGCT226280.1395115640215534No Hit
TAAGTAGAGTCTTCAGGAAAGGACGAAACACCGAACCAATACCAACTGGT225970.13932043539840205No Hit
TAAGTAGACTCTTCAGGAAAGGACGAAACACCGAACCAATACCAACTGGT225730.1391724648514462No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCACTACCAACTGGT223580.13784689536829992No Hit
TAAGTAGACTCTTGACGAAAGGACGAAACACCGAACCAATACCAACTGCT223400.13773591745808303No Hit
TAAGTAGACTCTTCTGGAAAGGACGAAACACCGAACCCATACCAACTGCT221110.13632403182254582No Hit
TAAGTACACTCTTCTGGAAAGGACGAAACACCGAACCAATACCAACTGCT218170.1345113926223365No Hit
TAAGTAGACTCTTGAGGAAAGGACGAAACACCGAACCACTACCAACTGCT217760.13425860960462022No Hit
TAAGTATACTCTTCAGGAAAGGACGAAACACCGAACCAATACCAACTGCT215780.13303785259223436No Hit
TAAGTATACTCTTCAGGAAAGGACGAAACACCGAACCACTACCAACTGCT215500.1328652202874525No Hit
TAAGTAAACTCTTCACGAAAGGACGAAACACCGAACCAATACCAACTGGT213850.13184792277713098No Hit
TAAGTAAACTCTTCAGCAAAGGACGAAACACCGAACCAATACCAACTGCT213810.131823261019305No Hit
TAAGTACAGTCTTCTGGAAAGGACGAAACACCGAACCAATACCAACTGCT211600.13046069889941975No Hit
TAAGTACACTCTTCTCCAAAGGACGAAACACCGAACCACTACCAACTGCT208080.12829046421073376No Hit
TAATTACACTCTTCACCAAAGGACGAAACACCGAACCAATACCAACTGCT206420.12726700126095566No Hit
TAAGTAAACTCTTCACGAAAGGACGAAACACCGAACCACTACCAACTGGT205780.12687241313574005No Hit
TAAGTATACTCTTCTCGAAAGGACGAAACACCGAACCAATACCAACTGCT205690.1268169241806316No Hit
TAAGTAGACTCTTCTCGAAAGGACGAAACACCGAACCAATACCAACTGGT201960.12451721526335921No Hit
TAAGTAGAGTCTTCTGGAAAGGACGAAACACCGAACCCCTACCAACTGCT201080.12397465659118771No Hit
TAAGTATACTCTTCTCGAAAGGACGAAACACCGAACCACTACCAACTGCT200580.12366638461836299No Hit
TAAGTAGACTCTTCTGGAAAGGACGAAACACCTAACCAATACCAACTGCT200090.12336427808499477No Hit
TAAGTAGAGTCTTCTCGAAAGGACGAAACACCGAACCAATACCAACTGGT199980.12329645825097334No Hit
TAAGTAGAGTCTTGACGAAAGGACGAAACACCGAACCAATACCAACTGCT199810.12319164578021294No Hit
TAATTACACTCTTCACGAAAGGACGAAACACCGAACCAATACCAACTGCT197850.12198321964674004No Hit
TAAGTAGACTCTTCTGGAAAGGACGAAACACCGAACCACTACCAACTGGT197400.1217057748711978No Hit
TAAGTAGAGTCTTCACGAAAGGACGAAACACCGAACCCCTACCAACTGCT193850.11951704386414233No Hit
TAAGTAGAGTCTTGTCGAAAGGACGAAACACCGAACCAATACCAACTGCT190370.11737147093328229No Hit
TAAGTAGACTCTTGAGGAAAGGACGAAACACCGAACCAATACCAACTGCT182980.112815211174933No Hit
TAAGTAGACTCTTCAGCAAAGGACGAAACACCGAACCCCTACCAACTGCT182970.11280904573547651No Hit
TAAGTACACTCTTCACGAAAGGACGAAACACCTAACCAATACCAACTGCT182770.1126857369463466No Hit
TAAGTACACTCTTCACCAAAGGACGAAACACCTAACCAATACCAACTGCT182500.11251927008102128No Hit
TAAGTAAACTCTTCAGCAAAGGACGAAACACCGAACCACTACCAACTGCT181430.11185956805917638No Hit
TAAGTAGAGTCTTCAGGAAAGGACGAAACACCGAACCCCTACCAACTGCT181360.11181640998298092No Hit
TAAGTAGACTCTTCACCAAAGGACGAAACACCGAACCACTACCAACTGGT179660.11076828527537688No Hit
TAAGTAAAGTCTTCACCAAAGGACGAAACACCGAACCAATACCAACTGCT178790.11023189204266187No Hit
TAAGTAGACTCTTGTGGAAAGGACGAAACACCGAACCACTACCAACTGCT178540.11007775605624953No Hit
TAAGTATAGTCTTCAGGAAAGGACGAAACACCGAACCACTACCAACTGCT178510.11005925973788004No Hit
TAAGTAGACTCTTCACCAAAGGACGAAACACCGAACCAATACCAACTGGT177290.10930707612418773No Hit
TAAGTAGACTCTTCAGCAAAGGACGAAACACCGAACCCATACCAACTGCT172450.10632300342724448No Hit
TAAGTAGAGTCTTCACGAAAGGACGAAACACCTAACCAATACCAACTGCT172390.10628601079050552No Hit
TAAGTATAGTCTTCTCGAAAGGACGAAACACCGAACCACTACCAACTGCT170950.10539818750877034No Hit
TAAGTAGAGTCTTCTCGAAAGGACGAAACACCGAACCCCTACCAACTGCT169030.10421442313312344No Hit
TAAGTAGAGTCTTCACGAAAGGACGAAACACCTAACCACTACCAACTGCT166560.10269155958736932No Hit
TAAGTATAGTCTTCAGGAAAGGACGAAACACCGAACCAATACCAACTGCT166330.10254975447986997No Hit
TAAGTAAAGTCTTCAGGAAAGGACGAAACACCGAACCACTACCAACTGCT166170.10245110744856606No Hit
TAAGTAAAGTCTTCAGGAAAGGACGAAACACCGAACCAATACCAACTGCT164600.10148313345389645No Hit
TAAGTAGACTCTTCTCCAAAGGACGAAACACCTAACCAATACCAACTGCT164490.101415313619875No Hit
TAATTACACTCTTCACGAAAGGACGAAACACCGAACCACTACCAACTGCT164030.10113170340487627No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)