FastQCFastQC Report
Mon 21 Nov 2022
H3TJ5BGXN_n01_R10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3TJ5BGXN_n01_R10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGTAGAGTCTTCTCCAAAGGACGAAACACCTGGGCAGCAAGAGCGCGG12.941176470588235No Hit
ATAAACGAACTCTAGACGAAAGGACGAAACACAGCAGAATAGCTTATTGA12.941176470588235No Hit
GATCGCTCGGTTCTAGAGGAAAGGACGAAACACCGGGTCGCAAACGAGAC12.941176470588235No Hit
ATACACCAACTCTACAGGAAAGGAGGAAACACCGAAGTGGTGAAGGATAT12.941176470588235No Hit
ATACACGATCTCTACTCGAAGGGACGAAACACAGTCGGAGTCAAAGACTT12.941176470588235No Hit
GATAGCTCGGTTCTACAGGAAAGGACGAAACACCGGACAAACGTGGGCGA12.941176470588235No Hit
GATAGCACCGTTCAACAGGAAAGGACGAAACACCGGCAGCAACCGGTTTA12.941176470588235No Hit
TAAGTAGACTCTTCACGAAAGGACGAAACACCGAGACAAACAAGGTGCCA12.941176470588235No Hit
GATAGCACCGTTCTTCAGGAAAGGACGAAACACCGGCTGTACGGAGTCGT12.941176470588235No Hit
GATAGCGCCGTTCTAGAGGAAAGGACGAAACACCGTCTACCTGCTGATAT12.941176470588235No Hit
CTAAACCAACTCTAGACGAAAGGACGAAACACAGACTAACCTAGCACGAT12.941176470588235No Hit
TAAGTAGACTCTTCAGGAAAGGACGAAACACCTACTGAAATTGAGAACGC12.941176470588235No Hit
TAAGTACAGTCTTCTGCAAAGGACGAAACACCGGGGCAGGTATGTCACAC12.941176470588235No Hit
TAAGTAGACTCTTGTGCAAAGGACGAAACACCGCAGCTTGCTGCAGAGAA12.941176470588235No Hit
TAAGTAGACTCTTCACCAAAGGACGAAACACCGGCGAATTATATTTAAGG12.941176470588235No Hit
ATACACGATCTCTACACGAAAGGACGAAACACAGGACTGGGACAGGAGAG12.941176470588235No Hit
TAAGTATACTCTTCAGGAAAGGACGAAACACCGCTCCTTGGGAGAGTCCT12.941176470588235No Hit
ATACACTATCTCTTCTCGAAAGGACGAAACTCAGCCTGGCTCAGAGGGAG12.941176470588235No Hit
TAATTATACTCTTGACCAAAGGACGAAACACCGTCTTCTCACTTTAATCT12.941176470588235No Hit
TAAGTAGACTCTTCACGAAAGGACGAAACACCGCCACAAATTGGCTGTCC12.941176470588235No Hit
GATAGCTCCGTTCTACAGGAAAGGACGAAACAACGCAGAATCTGGACTCA12.941176470588235No Hit
TAAGTAGACTCTTCACGAAAGGACGAAACACCGAGGATATCAGGATTCCA12.941176470588235No Hit
TAAGTACACTCTTCACGAAAGGACGAAACACCGCTAAAAGAGCCCCTGCA12.941176470588235No Hit
GATATCTCGGTTCTACAGGAAAGGACGAAACACCGTGTACACGTACTGTC12.941176470588235No Hit
TAAGTACACTCTTCACGAAAGGACGAAACACCTCTAAACGAGCCCCTGCA12.941176470588235No Hit
ATACACGAACTCTACAGGAAAGGACGAAACACAGGGATGAGGGACCTGAA12.941176470588235No Hit
ATACACTATCTCTACACGAAAGGACGAAACACAGCACATCATCCTCAGCT12.941176470588235No Hit
TAAGTACACTCTTGAGCAAAGGACGAAACACCGAGAATAGCTTATTGAGC12.941176470588235No Hit
ATACACGAACTCTACAGGAAAGGACGAAACACAGCGCTGCAGTGTGTGAC12.941176470588235No Hit
TAATTACACTCTTCACGAAAGGACGAAACACCTCCAAAGTAGCGCCAGCC12.941176470588235No Hit
TAAGTACAGTCTTCACCAAAGGACGAAACACCGACAACTTAACCTCGAGT12.941176470588235No Hit
TAAGTACACTCTTCACCAAAGGACGAAACACCGGCTGGCTTCATGATGGC12.941176470588235No Hit
TAAGTAGACTCTTCAGCAAAGGACGAAACACCTCCACAACATAACATCAA12.941176470588235No Hit
TAAGTAGAGTCTTCAGGAAAGGACGAAACACCGGAACTCCGAAGGCTTCC12.941176470588235No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)