FastQCFastQC Report
Mon 21 Nov 2022
H3TJ5BGXN_n01_R06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3TJ5BGXN_n01_R06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAAACGATCTCTTGTCGAAAGGACGAAACACAGAACACCTACCAACTGG12.941176470588235No Hit
GATCGCTCCGTTCTACAGGAAAGGACGAAACAACGCAATCTCCAGCTCTC12.941176470588235No Hit
TAAGTATACTCTTCACGAAAGGACGAAACACTTATCCAGGAAATAACAAC12.941176470588235No Hit
GATCGCGCGGTTCTAGAGGAAAGGACGAAACTACGATTGCTCGCTGGCCA12.941176470588235No Hit
GATAGCACGGTTCTACAGGAAAGGACGAAACAACGAACATGTTAAGTCGC12.941176470588235No Hit
ATACACTATCTCTTCAGGAAAAGACGAAACACCGCAAGATAGGAGACTCT12.941176470588235No Hit
TAAGTACACTCTTCACGAAAGGACGAAACACCGGACATTAAGAGGCCTTC12.941176470588235No Hit
ATACACGAACTCTACAGGAAAGGACGAAACACCGAACACCTACCAACTCG12.941176470588235No Hit
TATAGCTCCGTTCTACTGGAAAGGACGAAACACCGACGGCTTACGGGTCG12.941176470588235No Hit
ATACACGATCTCTACTGGAAAGGACGAAACACAGAACAACTACCAACTCC12.941176470588235No Hit
TAAGTACAGTCTTCACGAAAGGACGAAACACCGATAGGCGCACGGTCTAC12.941176470588235No Hit
GATAGCTCCGTTCTACAGGAAAGGACGAAACACCGGCATCGCGACGATCT12.941176470588235No Hit
ATACACGATCTCTACTCGAAAGGACGAAACACAGAACAACTACCAACTCC12.941176470588235No Hit
GATAGCCCCGTTCTACAGGAAAGGACGAAACAACGACTTCGGTGCTGCAT12.941176470588235No Hit
TAAGTAGAGTCTTCACGAAAGGACGAAACACCGGTAGAAATGGACGTTCA12.941176470588235No Hit
GATAGCACGGTTCTACAGGAAAGGACGAAACACCGACAGGGGCTCATCAT12.941176470588235No Hit
GATAGCTCCGTTCTACAGGAAAGGACGAAACACCGCGTGAGGAAGATCAT12.941176470588235No Hit
TAAGTATACTCTTCTCGAAAGGACGAAACACCGAGCCAAGGGAACCGGAG12.941176470588235No Hit
ATAAACAAACTCTAGACGAAAGGACGAAACACCGCGGACATTCGAGAGAA12.941176470588235No Hit
ATACACTAACTCTAGACGAAAGGACGAAACACAGAACAACTACCAACTGC12.941176470588235No Hit
TAAGTAAAGTCTTGACGAAAGGACGAAACACCGAAACAGTATGCAGCGAG12.941176470588235No Hit
GATAGCACCGTTCTTGAGGAAAGGACGAAACACCGGTCAGTGTTCGGGAC12.941176470588235No Hit
GATAGCACCGTTCTACAGGAAAGGACGAAACACCGTACATGCGGTTGACA12.941176470588235No Hit
ATAAACCAACTCTACACGAAAGGACGAAACACAGAACAACTACCAACTGC12.941176470588235No Hit
GATAGCTCCGTTCTACAGGAAAGGACGAAACACCGGTTCAGAAGTAAACC12.941176470588235No Hit
ATAAACTAACTCTAGACGAAAGGACGAAACACAGAACAACTACCAACTCC12.941176470588235No Hit
ATACACAAACTCTACACGAAAGGACGAAACACAGGCGTTCGCGAGTGCCT12.941176470588235No Hit
TAAGTAAACTCTTCACGAAAGGACGAAACACCGAGAGATGTGAGTCTGAT12.941176470588235No Hit
ATACACGATCTCTACACAAAAGAACGAAAACAACAAAAAACAACCAACTA12.941176470588235No Hit
GATAGCTCGGTTCTAGAGGAAAGGACGAAACACCGGTGATCCCAAAGGCA12.941176470588235No Hit
TAATTAAACTCTTCACGAAAGGACGAAACACCGGTCAAAGCACCAGGTCC12.941176470588235No Hit
TAAGTACACTCTTCACCAAAGGACGAAACACCGACGTAAGTTCGGCGTAC12.941176470588235No Hit
TAAGTAGACTCTTCACCAAAGGACGAAACACCGACGCTGTAGTGCCTTCG12.941176470588235No Hit
ATACACGATCTCTACACGAAAGGACGAAACACAGAACAACTACCAACTCC12.941176470588235No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)