FastQCFastQC Report
Mon 21 Nov 2022
H3TJ5BGXN_n01_R04rc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3TJ5BGXN_n01_R04rc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGTAGACTCTTCACCAAAGGACGAAACACCGAACCAATACCAACTGCT33.5294117647058822No Hit
TAAGTAGACTCTTCACGAAAGGACGAAACACCGAACCAATACCAACTGCT33.5294117647058822No Hit
TAAGTAGACTCTTCACCAAAGGACGAAACACCGAACCACTACCAACTGCT33.5294117647058822No Hit
TAAGTAGACTCTTCACGAAAGGACGAAACACCGAACCACTACCAACTGCT33.5294117647058822No Hit
TAAGTAGACTCTTCAGGAAAGGACGAAACACCGAACCACTACCAACTGCT22.3529411764705883No Hit
TAATTAAACTCTTCACGAAAGGACGAAACACCGAACCAATACCAACTGCT22.3529411764705883No Hit
TAAGTAGACTCTTCAGCAAAGGACGAAACACCGAACCAATACCAACTGCT11.1764705882352942No Hit
TAAGTACACTCTTCACGAAAGGACGAAACACCTAACCACTACCAACTGCA11.1764705882352942No Hit
TAAGTAGACTCTTCAGCAAAGGACGAAACACCTAACCACTACCAACTGCT11.1764705882352942No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCACTACCAACTGCT11.1764705882352942No Hit
TAAGTAGACTCTTCACCAAAGGACGAAACACCTAACCAATACCAACTGCT11.1764705882352942No Hit
TAAGTAGAGTCTTCACGAAAGGACGAAACACCGAACCACTACCAACTGCA11.1764705882352942No Hit
TAAGTATAGTCTTGAGGAAAGGACGAAACACCGAACCACTACCAACTGGT11.1764705882352942No Hit
TAAGTAGACTCTTCTCGAAAGGACGAAACACCGAACCACTACCAACTGCT11.1764705882352942No Hit
TAATTAGACTCTTCAGCAAAGGACGAAACACCGAACCACTACCAACTGCT11.1764705882352942No Hit
TAAGTAAAGTCTTCAGCAAAGGACGAAACACCGAACCACTACCAACTGCT11.1764705882352942No Hit
TAAGTACACTCTTCTGGAAAGGACGAAACACCGAACCACTACCAACTGTA11.1764705882352942No Hit
TAAGTAAACTCTTCTGCAAAGGACGAAACACCTAACCACTACCAACTGCA11.1764705882352942No Hit
TAATTAAACTCTTCACGAAAGGACGAAACACCGAACCACTACCAACTGCT11.1764705882352942No Hit
TAAGTAGACTCTTCTGCAAAGGACGAAACACCGAACCACTAGCAACTGGT11.1764705882352942No Hit
TAAGTAGAGTCTTCAGGAAAGGACGAAACACCGAACCAATACCAACTGCT11.1764705882352942No Hit
TAAGTAGAGTCTTCTGCAAAGGACGAAACACCGAACCACTACCAACTGCT11.1764705882352942No Hit
TAAGTATACTCTTCAGCAAAGGACGAAACACCGAACCCCTACCAACTGCT11.1764705882352942No Hit
TAAGTAGACTCTTCACGAAAGGACGAAACACCGAACCCATACCAACTGCT11.1764705882352942No Hit
GCTTGCACCGCTCAACTACAAAACTCAAAAAAAACAAAAACAAAACTCAA11.1764705882352942No Hit
TAAGTATAGTCTTCACGAAAGGACGAAACACCGAACCAATACCAACTGCT11.1764705882352942No Hit
TAAGTATAGTCTTCAGGAAAGGACGAAACACCGAACCACTACCAACTGCT11.1764705882352942No Hit
TAAGTACAGTCTTCAGGAAAGGACGAAACACCGAACCACTACCAACTGGT11.1764705882352942No Hit
TAAGTACAGTCTTGTCCAAAGGACGAAACACCGAACCACTACCAACTGGT11.1764705882352942No Hit
TAATTAGACTCTTCACCAAAGGACGAAACACCTAACCAATACCAACTGCA11.1764705882352942No Hit
TAATTATAGTCTTCACGAAAGGACGAAACACCGAACCAATACCAACTGCT11.1764705882352942No Hit
TAAGTAGAGTCTTGTCCAAAGGACGAAACACCTAACCCATACCAACTGCT11.1764705882352942No Hit
TAAGTACAGTCTTCAGCAAAGGACGAAACACCGAACCACTACCAACTGGT11.1764705882352942No Hit
TAAGTAGAGTCTTCAGGAAAGGACGAAACACCTAACCCATACCAACTGCT11.1764705882352942No Hit
TAAGTAGACTCTTCAGGAAAGGACGAAACACCGAACCCATACCAACTGCT11.1764705882352942No Hit
TAAGTATACTCTTCACGAAAGGACGAAACACCGAACCACTACCAACTGCT11.1764705882352942No Hit
TAAGTAGAGTCTTCTCGAAAGGACGAAACACCGAACCACTACCAACTGCT11.1764705882352942No Hit
TAAGTACACTCTTCACCAAAGGACGAACACCGAACCCATACCAAATGGTA11.1764705882352942No Hit
TAATTATAGTCTTCAGGAAAGGACGAAACACCGAACCACTACCAACTGCT11.1764705882352942No Hit
TAAGTAAACTCTTCACTAAAGGACGAAACACCTAACCACTACCAACTGCA11.1764705882352942No Hit
TAATTAAACTCTTCTCCAAAGGACGAAACACCTAACCCATACAAACTGCA11.1764705882352942No Hit
TAAGTAGACTCATCACGAAAGGACGAAACACCGAACCACTACCAAGTGCT11.1764705882352942No Hit
TAATTATACTCTTCACCATAGGACGAAACACCGAACCAATACCAACTGCT11.1764705882352942No Hit
TAATTACACTCTTCTGCAAAGGACGAAACACCTAACCAATACCAACTGCA11.1764705882352942No Hit
TAAGTAGAGTCTTCAGCAAAGGACGAAACACCGAACCACTACCAACTGCT11.1764705882352942No Hit
GAATTAAACTCTTGACAAAAGTACGAAAAACATAACAAATACCAACTGAT11.1764705882352942No Hit
TAAGTACACTCTTCAGCAAAGGACGAAACACCTAACCACTACCAACTGCT11.1764705882352942No Hit
TAAGTAGAGTCTTCAGGAAAGGACGAAACACCGAACCACTACCAACTGCT11.1764705882352942No Hit
TAAGTAGAGTCTTCACGAAAGGACGAAACACCGAACCCATACCAACTGCT11.1764705882352942No Hit
TAAGTATAGTCTTCTCGAAAGGACGAAACACCGAACCACTACCAACTGGT11.1764705882352942No Hit
TAATTAAACTCTTCACGAAAGGACGAAACACCTAACCACTACCAACTGCA11.1764705882352942No Hit
TAATTAGACTCTTCACGAAAGGACGAAACACCGAACCACTACCAACTGCT11.1764705882352942No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGCT11.1764705882352942No Hit
TAATTAGAGTCTTCTGGAAAGGACGAAACACCGAACCACTACCAACTGCT11.1764705882352942No Hit
TAAGTAGACTCTTCAGCAAAGGACGAAACACCGAACCACTACCAACTGGT11.1764705882352942No Hit
TAAGTAAAGTCTTCTCGAAAGGACGAAACACCGAACCACTACCAACTGCA11.1764705882352942No Hit
TAATTAAACTCTTCTCCAAAGGACGAAACACAGAACCACTACCAACTGCT11.1764705882352942No Hit
TAAGTAGAGTCTTCACCAAAGGACGAAACACCGAACCAATACAAACTGCT11.1764705882352942No Hit
TAAGTAGACTCTTCACGAAAGGACGAAACACCGTACCACTACCAACTGCT11.1764705882352942No Hit
TAATTACACTCTTCACGAAAGGACGAAACACCGAACCAATACCAACTGCT11.1764705882352942No Hit
TAATTAGAGTCTTCACCAAAGGACGAAACACCGAACCAATACCAACTGCT11.1764705882352942No Hit
TAAGTAGAGTCTTGAGGAAAGGACGAAACACCGAACCACTACCAACTGCT11.1764705882352942No Hit
TAAGTACAGTCTTCAGGAAAGGACGAAACACCGAACCAATACCAACTGCT11.1764705882352942No Hit
TAAGTAGACTCTTCACCAAAGGACGAAACACCTAACCCCTACCAACTGCT11.1764705882352942No Hit
TAAGTAGACTCTTCTGGAAAGGACGAAACACCGAACCAATACCAACTGCT11.1764705882352942No Hit
GAATTATAGTCTTCACTAAATTACGAAAAACCTAAAAAATAAAAAATGTT11.1764705882352942No Hit
TAATTACACTCTTCACCAAAGGACGAAACACCAAACCACTACCAACTGCA11.1764705882352942No Hit
TAATTAGACTCTTCTCGAAAGGACGAAAAACAGAACAAATACCAACTGCT11.1764705882352942No Hit
TAATTAAACTCTTCACCAAAGGACGAAACACCTAACCAATACCAACTGCT11.1764705882352942No Hit
TAAGTAGAGTCTTCACGAAAGGACGAAACACAGAACCAATACCAACTGGT11.1764705882352942No Hit
TAAGTACACTCTTGTGGAAAGGACGAAACACCGAACCACTACCAACTGGT11.1764705882352942No Hit
TAAGTACAGTCTTCAGGAAAGGACGAAACACCGAACCAATACCAACTGCA11.1764705882352942No Hit
TAAGTAAACTCTTCAGCAAAGGACGAAACACCGAACCCATACCAACTGCT11.1764705882352942No Hit
TAAGTAAACTCTTCACGAAAGGACGAAACACCTAACCAATACCAACTGGT11.1764705882352942No Hit
TAATTAAAGTCTTCACGAAAGGACGAAACACCTAACCAATACCAACTGGA11.1764705882352942No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)