FastQCFastQC Report
Mon 21 Nov 2022
H3TJ5BGXN_n01_R04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3TJ5BGXN_n01_R04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATAGCGCCGTTCTACAGGAAAGGACGAAACAACGGAGAGCATGAGGCCC13.125No Hit
ATAAACAAACTCTAGACGAAAGGACGAAACACAAAACGTGCCTCTTCACC13.125No Hit
GATAGCTCCGTTCTTGAGGAAAGGACGAAACACCGTTAAAGCCAAGTGTC13.125No Hit
GATAGCTCCGTTCTAGAGGAAAGGACGAAACACCGGTGGGACTTCCTGAG13.125No Hit
ATAAACAAACTCTAGACGAAAGGACGAAACACAGTAAGAGGAAGTCTGAC13.125No Hit
TAAGTACACTCTTCACCAAAGGACGAAACACCGTCGTATTGGTGGCGTGC13.125No Hit
ATACACTAACTCTACTCGAAAGGACGAAACACAGGAGACTCGACATATCT13.125No Hit
TAAGTACAGTCTTCAGGAAAGGACGAAACACCGGGAGTAGACGCCCCTGC13.125No Hit
ATACACGATCTCTACTCGAAAGGACGAAACACAGTCAAGCGGACGTTGTA13.125No Hit
ATACACAAACTCTACACGAAAGGACGAAACACAGGCGTATATAGAAGCAT13.125No Hit
ATAAACAAACTCTTCTCGAAAGGACGAAACACAGACCAACACACGGTTGC13.125No Hit
TAAGTACACTCTTCACGAAAGGACGAAACACCGTAACTGCCAACTGCTGA13.125No Hit
ATAAACAAACTCTACAGGAAAGGACGAAACACAGTGGATCATGGATTCCA13.125No Hit
GATAGCTCCGTTCTACAGGAAAGGACGAAACAACAGCGTTCGCGTGTGCC13.125No Hit
GATAGCGCGGTTCTACAGGAAAGGACGAAACACCGCGAAGCTCTGGAGTC13.125No Hit
ATACACGATCTCTAGACGAAAGGACGAAACACAGCAAGCCCAAATCCGAC13.125No Hit
ATAAACGATCTCTACTGGAAAGGACGAAACACAGAGGATGTTCCAGATCA13.125No Hit
GGGTGGAGCGGGGGCGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCC13.125No Hit
TAAGTAGAGTCTTCTGGAAAGGACGAAACACCGGGCCATGGAGGAGTCAC13.125No Hit
ATACACGATCTCTACACGAAAGGACGAAACACAGAGGGTAGCGATTCCTC13.125No Hit
GATAGCACGGTTCTAGAGGAAAGGACGAAACAACAAAGGCGCTGAAGCCA13.125No Hit
GATAGCACGGTTCTACAGGAAAGGACGAAACACCGCCTTAAGAAGCAGAC13.125No Hit
GATAGCACCGTTCATCTGGAAAGGACGAAACACCGGGGTGGCGCCCTCTG13.125No Hit
ATACACGAACTCTACACGAAAGGACGAAACACCGGCGTGCGGAGAGAGCA13.125No Hit
GATCGCGCGGTTCTTCAGGAAAGGACGAAACACCGGCGCTTCTACCAGAC13.125No Hit
TAAGTAGACTCTTCACGAAAGGACGAAACACCTCCAGAGACAAAAGGGAC13.125No Hit
GGGGGGAGCGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCC13.125No Hit
ATAAACTAACTCTAGTCGAAAGGACGAAACACAGAACGAGCTAGGAGCGC13.125No Hit
TAAGTAGAGTCTTCAGGAAAGGACGAAACACCTGTGCAAAACGTAGGGCA13.125No Hit
GAAGTAAACTCTTCAGGAAAGGACGAAACACCTCAGAATGGCAGCCGGCC13.125No Hit
GATAGCGCCGTTCTAGAGGAAAGGACGAAACACCGAGTATTCAGTGAAGA13.125No Hit
TAAGTACAGTCTTCACGAAAGGACGAAACACCGAAAAGATGCGCACGCCT13.125No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)