FastQCFastQC Report
Mon 21 Nov 2022
H3TJ5BGXN_n01_R03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3TJ5BGXN_n01_R03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAATTAAACTCTTCAGGAAAGGACGAAACACCGAACCACTACCAACTGCT12.631578947368421No Hit
GATAGCTCGGTTCTTGAGGAAAGGACGAAACAACGTGACTACTGTCGTTC12.631578947368421No Hit
TAAGTAGACTCTTCTCGAAAGGACGAAACACCGAACCACTACCAACTGCA12.631578947368421No Hit
ATACACGATCTCTAGTCGAAAGACGAAACACCTCATAGGGCGTCTTCTGA12.631578947368421No Hit
ATACACGATCTCTAGAGGAAAGGACGAAACACAGACAAGTCCAGCGGTGC12.631578947368421No Hit
TAATTATACTCTTGACGAAAGGACGAAACACCGAACCACTACCAACTGGT12.631578947368421No Hit
TAAGTAAAGTCTTCACGAAAGGACGAAACACCTAAGAATGAGTCTGTTCT12.631578947368421No Hit
TAATTAAACTCTTCTCGAAAGGACGAAACACCTAACCAATACCAACTGCA12.631578947368421No Hit
GGGTGGAGCGGGGCGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCC12.631578947368421No Hit
TAATTAAACTCTTCACGAAAGAAGAAAAACCGTACCCATACCAACTGGAA12.631578947368421No Hit
GATAGCTCCGTTCTTGAGGAAAGGACGAAACACCGCGTAGTGGATGAGAC12.631578947368421No Hit
TAAGTAAACTCTTCACGAAAGGACGAAACACCTCTGAAGGAAGGGCGGAT12.631578947368421No Hit
GATAGCTCGGTTCTTCAGGAAAGGACGAAACACCGGGAAGTTACAGTCAC12.631578947368421No Hit
TAAGTAGACTCTTCTCGAAAGGACGAAACACCGCGCCAATGGTGGCCGAC12.631578947368421No Hit
GATAGCGCCGTTCAACAGGAAAGGACGAAACACCAGGGAATGGAAAGCAG12.631578947368421No Hit
TAAGTAGACTCTTCTGGAAAGGACGAAACACCGAACCCCTACCAACTGCT12.631578947368421No Hit
TAATTAGACTCTTCTGGAATGCAGAAAACACCAAACCAAAACAAAATGGA12.631578947368421No Hit
GAATTAAACTCTTCAGCAAAGGACGAAACACCGAACCACTACCAACTGCT12.631578947368421No Hit
TAAGTACACTCTTCAGGAAAGGACGAAACACCGAACCACTACCAACTGCT12.631578947368421No Hit
TAAGTAGAGTCTTCTGGAAAGGACGAAACACCGAACCACTACCAACTGCT12.631578947368421No Hit
GATAGCTCCGTTCTAGAGGAAAGGACGAAACAACGACTTCTCCAGCCCTG12.631578947368421No Hit
GATAGCTCCGTTCTACTGGAAAGGACGAAACAACGGACAGTTTGCGGCTG12.631578947368421No Hit
TAATTATACTCTTCTGCAAAGGACGAAACACCGAACCAATCCCAACTGCA12.631578947368421No Hit
TAAGTAGACTCTTCACGAAAGGACGAAACACCGAACCACTACCAACTGCA12.631578947368421No Hit
GATAGCTCCGTTCTTCTGGAAAGGACGAAACAACGATTACGAGCAAGGGC12.631578947368421No Hit
TAAGTACAGTCTTCAGGAAAGGACGAAACACCGAACCAATACCAACTGCT12.631578947368421No Hit
TAAGTACACTCTTCAGCAAAGGACGAAACACCTAACCAATACCAACTGCT12.631578947368421No Hit
TAAGTAGACTCTTCAGGAAAGGACGAAACACCGAACCACTACCAACTGCT12.631578947368421No Hit
GATAGCACCGTTCTACAGGAAAGGACGAAACACCGGTTTCAGTGCGGGGC12.631578947368421No Hit
TAATTAGACTCTTCACCAAAGGACGAAACACCGAACCACTACCAACTGCT12.631578947368421No Hit
TAATTAAACTCTTCACCAAAGGACGAAACACCTAACCAATACCAACTGCT12.631578947368421No Hit
ATACACCAACTCTACACGAAAGGACGAAACACAGGGAAATGGGGCGCTCC12.631578947368421No Hit
TAATTAAACTCTTCACGAAAGGACGAAACACCGAACCAATACCAACTGCA12.631578947368421No Hit
TAATTACACTCTTCACCAAAGGACGAAACACCGAACCACTACCAACTGCT12.631578947368421No Hit
GATCGCGCGGTTCTAGTGGAAAGGACGAAACACCGCAATTTGTCTTTGCA12.631578947368421No Hit
TAATTAAACTCTTCTGCAAAGGACGAAACACCGAACCACTACCAACTGCA12.631578947368421No Hit
ATAAACTATCTCTAGTCGAAAGGACGAAACACAGATATAGAATGATGTCC12.631578947368421No Hit
TAATTACACTCTTCACCAAAGGACGAAACACCTAGCGATTACCGTCCTCC12.631578947368421No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)