FastQCFastQC Report
Tue 8 Nov 2022
H3TCYBGXN_n01_carly8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3TCYBGXN_n01_carly8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6729196
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTCACTGATCTCGTAT67994010.104327470919259TruSeq Adapter, Index 27 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTCACTGAGCTCGTAT404010.6003837605562388TruSeq Adapter, Index 27 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTCACTGATGTCGTAT298000.4428463667873547TruSeq Adapter, Index 27 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTCACTGCTCTCGTAT220460.3276171477246316TruSeq Adapter, Index 27 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTCACTGATATCGTAT159580.23714571547626193TruSeq Adapter, Index 27 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)