FastQCFastQC Report
Tue 8 Nov 2022
H3TCYBGXN_n01_carly14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3TCYBGXN_n01_carly14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2793988
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCATCTCGTAT1824566.530307216781174TruSeq Adapter, Index 15 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCATCGCGTAT243140.8702256416276662TruSeq Adapter, Index 15 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCATGTCGTAT115230.41242124160876853TruSeq Adapter, Index 15 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCAGCTCGTAT67540.2417333216892843TruSeq Adapter, Index 15 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCATCTCGTTT51660.18489700027344425TruSeq Adapter, Index 15 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCATCTCGGAT48700.17430282449316176TruSeq Adapter, Index 15 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCATATCGTAT29200.10451011242711135TruSeq Adapter, Index 15 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)