FastQCFastQC Report
Tue 8 Nov 2022
H3TCYBGXN_n01_TF4-MYB33-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3TCYBGXN_n01_TF4-MYB33-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6280384
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGATATCTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGCGATACT318340.5068798340993161No Hit
GGCAGACGTGCCCTCGGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAG249860.39784191539880365No Hit
CTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGCGATACTTGGTGTG245110.39027868359641704No Hit
CCCAGGCAGACGTGCCCTCGGCCAGAAGGCTTGGGGCGCAACTTGCGTTC198020.3152991918965464No Hit
CGCGAATCCTCTTGAAAGAGAGGGGTGCCTTCGGGAACGCGGACACAGGT183730.29254580611631387No Hit
CGGCAACGGATATCTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGC170590.2716235185619223No Hit
CCGATAGCGAACAAGTACCGCGAGGTAAAGATGAAAAGGACTTTGAAAAG156590.24933188798646708No Hit
GGCCGGTCCGCCTTTGGTGTGCATTGGTCGGCTTGTCCCTTCGGTCGGCG148880.23705556857669846No Hit
TGAAAATAAGCGTAGATCCGGAGATTCCCGAATAGGTTAACCTTTTGAAC145570.23178519020493013No Hit
CGAATGGGGAGCCCACAGGCCGACGCCCGGAGCACGCTGATGCCGAGGCA139060.22141958198734343No Hit
CAACGGGTGAGTAAACCCGTAAGGCGCAAGGAAGCTGATTGGCGGGATCC129850.2067548735873475No Hit
GTTGATTCGCACAATTGGTCATCGCGCTTGGTTGAAAAGCCAGTGGCGCG121860.19403272156606985No Hit
CTTACGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGTAAGCG99680.15871640969724143No Hit
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACT97970.15599364624838225No Hit
CAGACGTGCCCTCGGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAGAC84660.13480067460843156No Hit
GAAAATAAGCGTAGATCCGGAGATTCCCGAATAGGTTAACCTTTTGAACT82890.13198237559996331No Hit
CTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTC81180.12925961215110413No Hit
CTGCAAAGGATTCTACCCGCCGCTCGGTGGTAATTATAATTCAAGGCGGT81000.12897300547227686No Hit
CGGGTGAGTAAACCCGTAAGGCGCAAGGAAGCTGATTGGCGGGATCCCTC79780.12703044909355862No Hit
GCTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATT79500.1265846164820495No Hit
CCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATA78740.12537449939366765No Hit
AAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGCGAACCGGGAAGAG78380.12480128603601308No Hit
GTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAAGCTGATTGG77680.12368670450724033No Hit
GGTAATTATAATTCAAGGCGGTCCGAACGACGCTTCCGCCGAACGGACTT75530.12026334695458112No Hit
GTCGGCTTGTCCCTTCGGTCGGCGATACGCTCCTGGTCTTAATTGGCCGG72850.11599609195870825No Hit
GGGAAACTTCGGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGC71510.11386246446077183No Hit
GGCTTACGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGTAAG69470.11061425543406261No Hit
GTTGAAAATAAGCGTAGATCCGGAGATTCCCGAATAGGTTAACCTTTTGA68430.10895830573417167No Hit
CGGATGGGGGCCGGCGATGCGTCCTGGTCGGATGCGGAACGGAGCAATCC68290.10873538942841711No Hit
GTCGTCTGCAAAGGATTCTACCCGCCGCTCGGTGGTAATTATAATTCAAG67920.10814625347749438No Hit
CTCGGAGAGAGGCTCGGTGAAATAGACATGTCTGTGAAGATGCGGACTAC66050.10516872853634426No Hit
CCGAAGGCCAACACAATAGGATCGAAATCCTATGATGTTATCCCATGCTA64860.10327393993743057No Hit
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAAC63710.1014428417115896No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)