FastQCFastQC Report
Tue 8 Nov 2022
H3TCYBGXN_n01_TF3-GBF4-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3TCYBGXN_n01_TF3-GBF4-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7022239
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGCGATACTTGGTGTG941881.34128160548224No Hit
CGGATATCTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGCGATACT674900.9610894758779928No Hit
CCCAGGCAGACGTGCCCTCGGCCAGAAGGCTTGGGGCGCAACTTGCGTTC613880.8741941138716582No Hit
GGCAGACGTGCCCTCGGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAG603560.8594979464526913No Hit
CGGCAACGGATATCTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGC318140.4530463859176539No Hit
CGCGAATCCTCTTGAAAGAGAGGGGTGCCTTCGGGAACGCGGACACAGGT281690.4011398643651975No Hit
CAGACGTGCCCTCGGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAGAC214500.3054581309465542No Hit
CTTACGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGTAAGCG212780.30300876971005974No Hit
CAACGGGTGAGTAAACCCGTAAGGCGCAAGGAAGCTGATTGGCGGGATCC198860.28318603226122036No Hit
CTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAA177730.2530959142803314No Hit
CTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTC170640.2429994194159441No Hit
CTCGGAGAGAGGCTCGGTGAAATAGACATGTCTGTGAAGATGCGGACTAC138600.1973729461500812No Hit
CCGATAGCGAACAAGTACCGCGAGGTAAAGATGAAAAGGACTTTGAAAAG135070.19234605942634536No Hit
CTTTCTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAGGTTGTCTCT134960.19218941423098815No Hit
AAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGCGAACCGGGAAGAG131770.18764670356562915No Hit
CAGGCAGACGTGCCCTCGGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAA127250.18121001008367843No Hit
TGAAAATAAGCGTAGATCCGGAGATTCCCGAATAGGTTAACCTTTTGAAC127160.18108184583293163No Hit
CGAATGGGGAGCCCACAGGCCGACGCCCGGAGCACGCTGATGCCGAGGCA124930.1779062205088719No Hit
CTGGCGTCGAGCTATTTTTCCGCAGGACCTCCCCTACAGTATCGTCACCG113590.16175752491477433No Hit
CCAGGCAGACGTGCCCTCGGCCAGAAGGCTTGGGGCGCAACTTGCGTTCA112540.16026227532272827No Hit
GTCTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGG99530.1417354208536622No Hit
CCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATA95560.13608195334849754No Hit
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACT94840.1350566393425231No Hit
CTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGA94350.13435885619956825No Hit
CTGCAAAGGATTCTACCCGCCGCTCGGTGGTAATTATAATTCAAGGCGGT92550.13179557118463214No Hit
CGGGTGAGTAAACCCGTAAGGCGCAAGGAAGCTGATTGGCGGGATCCCTC92030.1310550666247617No Hit
CTCGAAGACGATCAGATACCGTCCTAGTCTCAACCATAAACGATGCCGAC91310.13002975261878724No Hit
CTTACCAGGTCCAGACATAGTAAGGATTGACAGACTGAGAGCTCTTTCTT87850.12510254920118782No Hit
GGATATCTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGCGATACTT86850.12367850197066776No Hit
CACGCTTTCACGGTTCGTATTCGTACTGAAAATCAGAATCAAACGAGCTT85380.12158515254180327No Hit
GTTGATTCGCACAATTGGTCATCGCGCTTGGTTGAAAAGCCAGTGGCGCG84710.12063104089735481No Hit
CTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGC81900.11662946817959344No Hit
CAGGGTTCCAAACTCAACGGTGGCAACTAAACACGAGGGTTGCGCTCGTT81630.11624497542735301No Hit
CTCACACTCGAACCCTTCTCAGAAGATCAAGGTCGGTCGGCGGTGCACCC80110.11408042363696251No Hit
GGCCGGTCCGCCTTTGGTGTGCATTGGTCGGCTTGTCCCTTCGGTCGGCG79390.11305510963098807No Hit
CAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTAGG78980.11247125026647484No Hit
CTTTTATCTAATAAATGCGTCCCTTCCATAAGTCGGGGTTTGTTGCACGT77560.11044910319913635No Hit
AAAGGATTCTACCCGCCGCTCGGTGGTAATTATAATTCAAGGCGGTCCGA77390.11020701516994792No Hit
ATCTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGCGATACTTGGTG76990.10963739627773991No Hit
AAATAAGCGTAGATCCGGAGATTCCCGAATAGGTTAACCTTTTGAACTGC76220.10854087991023945No Hit
CTTTTCTGTTTAACAGCCTGCCCACCCTGGAAACGGCTCAGCCGGAGGTA75010.10681778276131018No Hit
CTAAACACGAGGGTTGCGCTCGTTGCGGGACTTAACCCAACACCTTACGG74840.10657569473212178No Hit
CCAAGCTTGAGTACGGTAGGGGCAGAGGGAATTTCCGGTGGAGCGGTGAA74750.10644753048137497No Hit
CAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGTGAGCTGGGTTCAGA74090.10550765930923171No Hit
CAAATATTCAAATGAGAACTTTGAAGGCCGAAGAGGGGAAAGGTTCCATG73210.10425449774637406No Hit
GTTGAAAATAAGCGTAGATCCGGAGATTCCCGAATAGGTTAACCTTTTGA72700.10352823365880881No Hit
CTCATCTTGGGGTGGGCTTACTACTTAGATGCTTTCAGCAGTTATCCGCT72490.1032291837403996No Hit
GTGGAAACTACCAAGCTTGAGTACGGTAGGGGCAGAGGGAATTTCCGGTG71930.10243171729130837No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)