FastQCFastQC Report
Tue 8 Nov 2022
H3TCYBGXN_n01_TF3-GBF4-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3TCYBGXN_n01_TF3-GBF4-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5964043
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGCGATACTTGGTGTG528150.8855569954810856No Hit
CGGATATCTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGCGATACT470940.789632133772342No Hit
GGCAGACGTGCCCTCGGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAG431820.7240390453254613No Hit
CCCAGGCAGACGTGCCCTCGGCCAGAAGGCTTGGGGCGCAACTTGCGTTC344590.5777792011224601No Hit
CGGCAACGGATATCTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGC277300.46495305282004173No Hit
CGCGAATCCTCTTGAAAGAGAGGGGTGCCTTCGGGAACGCGGACACAGGT195200.32729475625846427No Hit
CCGATAGCGAACAAGTACCGCGAGGTAAAGATGAAAAGGACTTTGAAAAG155200.26022615866451665No Hit
CAGACGTGCCCTCGGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAGAC144160.24171522572858714No Hit
AAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGCGAACCGGGAAGAG135050.22644035262656556No Hit
CAACGGGTGAGTAAACCCGTAAGGCGCAAGGAAGCTGATTGGCGGGATCC131630.22070598753228307No Hit
CTTACGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGTAAGCG114990.19280545093320084No Hit
CTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTC112440.1885298278365867No Hit
CTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAA110180.18474045207252865No Hit
CGAATGGGGAGCCCACAGGCCGACGCCCGGAGCACGCTGATGCCGAGGCA109320.18329847722425877No Hit
TGAAAATAAGCGTAGATCCGGAGATTCCCGAATAGGTTAACCTTTTGAAC98910.1658438747004339No Hit
CCGGGCACGACGGGGCTCTCACCCTCTCTGGCGCCCCTTTCCAGGGAACT90870.15236308658405046No Hit
GTTGATTCGCACAATTGGTCATCGCGCTTGGTTGAAAAGCCAGTGGCGCG89100.14939530114051827No Hit
CTCGGAGAGAGGCTCGGTGAAATAGACATGTCTGTGAAGATGCGGACTAC89070.1493449996923228No Hit
CCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATA86900.14570652827285116No Hit
CAGGCAGACGTGCCCTCGGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAA86560.1451364451933026No Hit
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACT86180.1444992935161601No Hit
GGCCGGTCCGCCTTTGGTGTGCATTGGTCGGCTTGTCCCTTCGGTCGGCG81090.13596481447233025No Hit
CCAGGCAGACGTGCCCTCGGCCAGAAGGCTTGGGGCGCAACTTGCGTTCA79780.13376831790112848No Hit
CTGGCGTCGAGCTATTTTTCCGCAGGACCTCCCCTACAGTATCGTCACCG79300.1329634947300011No Hit
CTGCAAAGGATTCTACCCGCCGCTCGGTGGTAATTATAATTCAAGGCGGT78680.13192393146729492No Hit
GTCTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGG77080.12924118756353703No Hit
CTTTCTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAGGTTGTCTCT75850.12717882818752313No Hit
CGGGTGAGTAAACCCGTAAGGCGCAAGGAAGCTGATTGGCGGGATCCCTC69050.11577716659655203No Hit
GGTAATTATAATTCAAGGCGGTCCGAACGACGCTTCCGCCGAACGGACTT64620.10834931941302234No Hit
AAAGGATTCTACCCGCCGCTCGGTGGTAATTATAATTCAAGGCGGTCCGA64530.10819841506843596No Hit
CAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGTGAGCTGGGTTCAGA64230.10769540058648136No Hit
GGCATGATGACTTGACGTCATCCTCACCTTCCTCCGGCTTATCACCGGCA63350.10621989143941452No Hit
GCTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATT63110.10581747985385082No Hit
GGATATCTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGCGATACTT63030.10568334265866292No Hit
GTGGAAACTACCAAGCTTGAGTACGGTAGGGGCAGAGGGAATTTCCGGTG60010.10061966354031988No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)