FastQCFastQC Report
Tue 8 Nov 2022
H3TCYBGXN_n01_TF2-ABF2-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3TCYBGXN_n01_TF2-ABF2-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4149229
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGATATCTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGCGATACT234690.5656231555308227No Hit
CTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGCGATACTTGGTGTG209760.5055397038823357No Hit
GGCAGACGTGCCCTCGGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAG196210.4728830344143454No Hit
CCCAGGCAGACGTGCCCTCGGCCAGAAGGCTTGGGGCGCAACTTGCGTTC161850.3900724688851832No Hit
CGCGAATCCTCTTGAAAGAGAGGGGTGCCTTCGGGAACGCGGACACAGGT145920.35167979400510313No Hit
CGGCAACGGATATCTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGC122480.29518737095494124No Hit
CCGATAGCGAACAAGTACCGCGAGGTAAAGATGAAAAGGACTTTGAAAAG101390.2443586507276412No Hit
GGCCGGTCCGCCTTTGGTGTGCATTGGTCGGCTTGTCCCTTCGGTCGGCG88720.21382285721033958No Hit
TGAAAATAAGCGTAGATCCGGAGATTCCCGAATAGGTTAACCTTTTGAAC87940.21194298989041094No Hit
CGAATGGGGAGCCCACAGGCCGACGCCCGGAGCACGCTGATGCCGAGGCA82670.19924183504935497No Hit
CAACGGGTGAGTAAACCCGTAAGGCGCAAGGAAGCTGATTGGCGGGATCC82210.1981331953478586No Hit
CTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTC81230.19577131076640983No Hit
AAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGCGAACCGGGAAGAG76660.18475721634067438No Hit
CTTACGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGTAAGCG76330.18396188785916615No Hit
GTTGATTCGCACAATTGGTCATCGCGCTTGGTTGAAAAGCCAGTGGCGCG72770.17538198060410742No Hit
CCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATA71750.17292369257035464No Hit
CAGACGTGCCCTCGGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAGAC62920.1516426304742399No Hit
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACT60640.14614763369291017No Hit
GCTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATT56170.13537454789793477No Hit
GGTAATTATAATTCAAGGCGGTCCGAACGACGCTTCCGCCGAACGGACTT53590.1291565252243248No Hit
CTGCAAAGGATTCTACCCGCCGCTCGGTGGTAATTATAATTCAAGGCGGT52340.12614391733982386No Hit
CTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAA52150.12568600094137972No Hit
GTCTGATAGCGCTTAAGCGCGAACTTCGAAAGGGGATCCGGTTAAAATTC50860.12257698960457473No Hit
CTCGGAGAGAGGCTCGGTGAAATAGACATGTCTGTGAAGATGCGGACTAC49920.12031150847543001No Hit
GTCGGCTTGTCCCTTCGGTCGGCGATACGCTCCTGGTCTTAATTGGCCGG49540.11939567567854173No Hit
CGGGTGAGTAAACCCGTAAGGCGCAAGGAAGCTGATTGGCGGGATCCCTC49260.11872085151241352No Hit
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAAC48830.11768451440014517No Hit
GAAAATAAGCGTAGATCCGGAGATTCCCGAATAGGTTAACCTTTTGAACT47890.11541903327100048No Hit
GTTGAAAATAAGCGTAGATCCGGAGATTCCCGAATAGGTTAACCTTTTGA44520.10729704241438591No Hit
GTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAAGCTGATTGG43690.10529667077907726No Hit
AAAGGATTCTACCCGCCGCTCGGTGGTAATTATAATTCAAGGCGGTCCGA43580.10503156128524119No Hit
CTTTCTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAGGTTGTCTCT43280.10430853539296096No Hit
AAATAAGCGTAGATCCGGAGATTCCCGAATAGGTTAACCTTTTGAACTGC43200.10411572848835289No Hit
GTCTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGG42640.10276608015609646No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA42020.101271826645384No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)