FastQCFastQC Report
Tue 8 Nov 2022
H3TCYBGXN_n01_TF2-ABF2-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3TCYBGXN_n01_TF2-ABF2-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4722514
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGATATCTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGCGATACT308400.6530420026282611No Hit
CTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGCGATACTTGGTGTG274830.5819569830814689No Hit
GGCAGACGTGCCCTCGGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAG248010.5251651980279994No Hit
CCCAGGCAGACGTGCCCTCGGCCAGAAGGCTTGGGGCGCAACTTGCGTTC195050.4130215389514991No Hit
CGCGAATCCTCTTGAAAGAGAGGGGTGCCTTCGGGAACGCGGACACAGGT155390.3290408456173979No Hit
CGGCAACGGATATCTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGC142210.3011319818215467No Hit
TGAAAATAAGCGTAGATCCGGAGATTCCCGAATAGGTTAACCTTTTGAAC109740.23237623011811084No Hit
CAACGGGTGAGTAAACCCGTAAGGCGCAAGGAAGCTGATTGGCGGGATCC98180.20789774260065721No Hit
GGCCGGTCCGCCTTTGGTGTGCATTGGTCGGCTTGTCCCTTCGGTCGGCG96370.20406503824022545No Hit
CCGATAGCGAACAAGTACCGCGAGGTAAAGATGAAAAGGACTTTGAAAAG91220.19315982970087542No Hit
CAGACGTGCCCTCGGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAGAC86070.1822546211615254No Hit
AAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGCGAACCGGGAAGAG84580.17909952199188822No Hit
GTTGATTCGCACAATTGGTCATCGCGCTTGGTTGAAAAGCCAGTGGCGCG84090.17806193904348405No Hit
CGAATGGGGAGCCCACAGGCCGACGCCCGGAGCACGCTGATGCCGAGGCA82580.1748644895494222No Hit
CTTACGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGTAAGCG81800.17321282689686043No Hit
CTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTC66930.14172536068712555No Hit
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACT63760.1350128342658169No Hit
CTCGGAGAGAGGCTCGGTGAAATAGACATGTCTGTGAAGATGCGGACTAC62170.1316459834740564No Hit
GAAAATAAGCGTAGATCCGGAGATTCCCGAATAGGTTAACCTTTTGAACT61230.12965551822609736No Hit
CCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATA61040.12925319014406308No Hit
CGGGTGAGTAAACCCGTAAGGCGCAAGGAAGCTGATTGGCGGGATCCCTC59190.12533578513478202No Hit
GCTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATT56780.12023257104161046No Hit
CAGGCAGACGTGCCCTCGGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAA54680.11578578697702113No Hit
GTTGAAAATAAGCGTAGATCCGGAGATTCCCGAATAGGTTAACCTTTTGA54170.1147058537041923No Hit
GTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAAGCTGATTGG53980.11430352562215804No Hit
GGTAATTATAATTCAAGGCGGTCCGAACGACGCTTCCGCCGAACGGACTT51450.10894620958243852No Hit
GTCTGATAGCGCTTAAGCGCGAACTTCGAAAGGGGATCCGGTTAAAATTC50620.10718867111881511No Hit
CTGCAAAGGATTCTACCCGCCGCTCGGTGGTAATTATAATTCAAGGCGGT50530.10699809465890414No Hit
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAAC50170.10623578881926024No Hit
AAATAAGCGTAGATCCGGAGATTCCCGAATAGGTTAACCTTTTGAACTGC49380.10456295100448618No Hit
CTTTCTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAGGTTGTCTCT48680.10308068964962305No Hit
GGCTTACGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGTAAG47530.10064554599520509No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)