FastQCFastQC Report
Tue 8 Nov 2022
H3TCYBGXN_n01_TF1-DIV1-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3TCYBGXN_n01_TF1-DIV1-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5500254
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGATATCTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGCGATACT264060.48008691962225747No Hit
CTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGCGATACTTGGTGTG213260.38772754858230185No Hit
GGCAGACGTGCCCTCGGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAG200360.3642740862512895No Hit
CCCAGGCAGACGTGCCCTCGGCCAGAAGGCTTGGGGCGCAACTTGCGTTC173070.3146581957851401No Hit
CCGATAGCGAACAAGTACCGCGAGGTAAAGATGAAAAGGACTTTGAAAAG167470.3044768477964836No Hit
GGCCGGTCCGCCTTTGGTGTGCATTGGTCGGCTTGTCCCTTCGGTCGGCG156680.28485957193976863No Hit
CGCGAATCCTCTTGAAAGAGAGGGGTGCCTTCGGGAACGCGGACACAGGT147460.2680967097155877No Hit
CGAATGGGGAGCCCACAGGCCGACGCCCGGAGCACGCTGATGCCGAGGCA136890.24887941538699848No Hit
TGAAAATAAGCGTAGATCCGGAGATTCCCGAATAGGTTAACCTTTTGAAC135090.24560683924778745No Hit
CAACGGGTGAGTAAACCCGTAAGGCGCAAGGAAGCTGATTGGCGGGATCC130860.23791628532064155No Hit
CGGCAACGGATATCTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGC123790.22506233348496268No Hit
GTTGATTCGCACAATTGGTCATCGCGCTTGGTTGAAAAGCCAGTGGCGCG116700.2121720196921815No Hit
CTTACGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGTAAGCG106460.19355469765578098No Hit
CTGCAAAGGATTCTACCCGCCGCTCGGTGGTAATTATAATTCAAGGCGGT97410.17710091206696998No Hit
GGTAATTATAATTCAAGGCGGTCCGAACGACGCTTCCGCCGAACGGACTT88780.16141072757730826No Hit
CTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTC84690.15397470734987875No Hit
CCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATA82040.14915674803381807No Hit
GTCGTCTGCAAAGGATTCTACCCGCCGCTCGGTGGTAATTATAATTCAAG76630.1393208386376338No Hit
CGGGTGAGTAAACCCGTAAGGCGCAAGGAAGCTGATTGGCGGGATCCCTC76310.1387390473239963No Hit
GTCGGCTTGTCCCTTCGGTCGGCGATACGCTCCTGGTCTTAATTGGCCGG76030.13822997992456348No Hit
GAAAATAAGCGTAGATCCGGAGATTCCCGAATAGGTTAACCTTTTGAACT74740.13588463369146225No Hit
GCTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATT74100.13472105106418722No Hit
GTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAAGCTGATTGG71900.1307212357829293No Hit
CTGCAGACCACGATCACGGCAGCGACGTCTCCACAAGCGTATCAAAAGCC70680.12850315639968626No Hit
AAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGCGAACCGGGAAGAG69620.126575972673262No Hit
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACT69230.12586691450976628No Hit
CAGACGTGCCCTCGGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAGAC68090.12379428295493262No Hit
GTTGAAAATAAGCGTAGATCCGGAGATTCCCGAATAGGTTAACCTTTTGA67260.12228526173518532No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA64560.11737639752636879No Hit
CCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAA64210.11674006327707775No Hit
AAAGGATTCTACCCGCCGCTCGGTGGTAATTATAATTCAAGGCGGTCCGA62970.11448562193673238No Hit
CTCGGAGAGAGGCTCGGTGAAATAGACATGTCTGTGAAGATGCGGACTAC61670.11212209472507997No Hit
CCGAAGGCCAACACAATAGGATCGAAATCCTATGATGTTATCCCATGCTA61210.11128576971172605No Hit
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAAC61200.11126758873317487No Hit
GGCTTACGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGTAAG61030.11095851209780494No Hit
GTCTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGG60580.11014036806300219No Hit
AAATAAGCGTAGATCCGGAGATTCCCGAATAGGTTAACCTTTTGAACTGC59170.10757685008728687No Hit
GTCGAATGGGGAGCCCACAGGCCGACGCCCGGAGCACGCTGATGCCGAGG58220.1058496571249255No Hit
GGGAAAGGTTCCATGTGAACGGCACTTGCACATGGGTTAGTCGATCCTAA58010.10546785657535088No Hit
GTCCGCCTTTGGTGTGCATTGGTCGGCTTGTCCCTTCGGTCGGCGATACG57610.10474061743330398No Hit
CTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGC57280.10414064514111529No Hit
CTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAA56980.10359521578458013No Hit
GTTCGATTAGTCTTTCGCCCCTATACCCAAGTCAGACGAACGATTTGCAC56980.10359521578458013No Hit
ATTCAACCTAGTACGAGAGGAACCGTTGATTCGCACAATTGGTCATCGCG56550.10281343370687972No Hit
GCCGGTCCGCCTTTGGTGTGCATTGGTCGGCTTGTCCCTTCGGTCGGCGA55710.10128623150858125No Hit
CTTTCTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAGGTTGTCTCT55560.10101351683031365No Hit
CGTTGATTCGCACAATTGGTCATCGCGCTTGGTTGAAAAGCCAGTGGCGC55510.1009226119375578No Hit
GGGAAACTTCGGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGC55240.10043172551667615No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)