FastQCFastQC Report
Tue 8 Nov 2022
H3TCYBGXN_n01_TF1-DIV1-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3TCYBGXN_n01_TF1-DIV1-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12075381
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGATATCTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGCGATACT368000.3047522889753955No Hit
TGAAAATAAGCGTAGATCCGGAGATTCCCGAATAGGTTAACCTTTTGAAC310660.2572672448181966No Hit
CTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGCGATACTTGGTGTG292680.24237744548184442No Hit
AAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGCGAACCGGGAAGAG289840.2400255528169256No Hit
GGCCGGTCCGCCTTTGGTGTGCATTGGTCGGCTTGTCCCTTCGGTCGGCG282440.23389738178861602No Hit
CCGATAGCGAACAAGTACCGCGAGGTAAAGATGAAAAGGACTTTGAAAAG265100.21953758643309063No Hit
CGAATGGGGAGCCCACAGGCCGACGCCCGGAGCACGCTGATGCCGAGGCA264390.2189496132668609No Hit
CGCGAATCCTCTTGAAAGAGAGGGGTGCCTTCGGGAACGCGGACACAGGT263520.2182291391054245No Hit
GGCAGACGTGCCCTCGGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAG255210.21134736866687687No Hit
GTTGATTCGCACAATTGGTCATCGCGCTTGGTTGAAAAGCCAGTGGCGCG228760.1894432978967703No Hit
CCCAGGCAGACGTGCCCTCGGCCAGAAGGCTTGGGGCGCAACTTGCGTTC226110.18724875016365944No Hit
GAAAATAAGCGTAGATCCGGAGATTCCCGAATAGGTTAACCTTTTGAACT184290.15261630254150987No Hit
CAACGGGTGAGTAAACCCGTAAGGCGCAAGGAAGCTGATTGGCGGGATCC179940.1490139317343279No Hit
CTGCAAAGGATTCTACCCGCCGCTCGGTGGTAATTATAATTCAAGGCGGT174250.14430186509228984No Hit
CCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATA172740.14305138695002667No Hit
GGTAATTATAATTCAAGGCGGTCCGAACGACGCTTCCGCCGAACGGACTT170430.14113840383173004No Hit
GAAACTAACAAGGATTCCCTTAGTAACGGCGAGCGAACCGGGAAGAGCCC169140.14007011455787607No Hit
GCTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATT167300.1385463531129991No Hit
GTTGAAAATAAGCGTAGATCCGGAGATTCCCGAATAGGTTAACCTTTTGA152720.1264721999247891No Hit
CTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTC151200.12521344047032554No Hit
GTCGTCTGCAAAGGATTCTACCCGCCGCTCGGTGGTAATTATAATTCAAG149180.12354061540584102No Hit
CCGGGCACGACGGGGCTCTCACCCTCTCTGGCGCCCCTTTCCAGGGAACT147810.1224060756343837No Hit
CGGCAACGGATATCTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGC147440.12209966708296824No Hit
GGAAAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGCGAACCGGGAA145950.12086575156510589No Hit
CTTACGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGTAAGCG144380.11956558554964021No Hit
GTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAAGCTGATTGG129330.1071022106880106No Hit
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAAC129010.10683720869759719No Hit
CTTTCTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAGGTTGTCTCT126790.10499875738910433No Hit
AAACTAACAAGGATTCCCTTAGTAACGGCGAGCGAACCGGGAAGAGCCCA126660.1048911003304989No Hit
CGTTGATTCGCACAATTGGTCATCGCGCTTGGTTGAAAAGCCAGTGGCGC126400.10467578621328802No Hit
CGCTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGAT124650.1032265565782148No Hit
GTTCGATTAGTCTTTCGCCCCTATACCCAAGTCAGACGAACGATTTGCAC123400.10219139255316251No Hit
GTCGAATGGGGAGCCCACAGGCCGACGCCCGGAGCACGCTGATGCCGAGG121630.10072560029368845No Hit
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACT121450.10057653667408092No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)