FastQCFastQC Report
Tue 8 Nov 2022
H3TCYBGXN_n01_EV-phd-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3TCYBGXN_n01_EV-phd-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6441575
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGATATCTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGCGATACT166040.25776304708087694No Hit
CGAATGGGGAGCCCACAGGCCGACGCCCGGAGCACGCTGATGCCGAGGCA159250.24722214675758644No Hit
GGCCGGTCCGCCTTTGGTGTGCATTGGTCGGCTTGTCCCTTCGGTCGGCG147710.22930727345408536No Hit
CTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGCGATACTTGGTGTG132330.20543112515184564No Hit
GGCAGACGTGCCCTCGGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAG115740.1796765542588575No Hit
CCGATAGCGAACAAGTACCGCGAGGTAAAGATGAAAAGGACTTTGAAAAG109120.16939956454749033No Hit
TGAAAATAAGCGTAGATCCGGAGATTCCCGAATAGGTTAACCTTTTGAAC105430.16367115185338987No Hit
GTTGATTCGCACAATTGGTCATCGCGCTTGGTTGAAAAGCCAGTGGCGCG101270.15721310393808968No Hit
CAACGGGTGAGTAAACCCGTAAGGCGCAAGGAAGCTGATTGGCGGGATCC98870.153487307063878No Hit
AAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGCGAACCGGGAAGAG97140.15080162848371712No Hit
CTGCAAAGGATTCTACCCGCCGCTCGGTGGTAATTATAATTCAAGGCGGT93630.14535265055518254No Hit
CGCGAATCCTCTTGAAAGAGAGGGGTGCCTTCGGGAACGCGGACACAGGT88810.13787000849947412No Hit
CCCAGGCAGACGTGCCCTCGGCCAGAAGGCTTGGGGCGCAACTTGCGTTC88510.13740428389019765No Hit
CCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATA87550.13591396514051302No Hit
GGTAATTATAATTCAAGGCGGTCCGAACGACGCTTCCGCCGAACGGACTT85310.1324365547245821No Hit
GTCGGCTTGTCCCTTCGGTCGGCGATACGCTCCTGGTCTTAATTGGCCGG79950.12411560837217606No Hit
CTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTC79350.12318415915362314No Hit
GTCGTCTGCAAAGGATTCTACCCGCCGCTCGGTGGTAATTATAATTCAAG73030.11337289405153243No Hit
GCTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATT71640.11121503669521816No Hit
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAAC70840.10997310440381428No Hit
CTTTTCTGTTTAACAGCCTGCCCACCCTGGAAACGGCTCAGCCGGAGGTA68010.10557976892297304No Hit
CGGCAACGGATATCTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGC67980.10553319646204538No Hit
GTTCGATTAGTCTTTCGCCCCTATACCCAAGTCAGACGAACGATTTGCAC67860.1053469066183348No Hit
TCCAGCTCCAATAGCGTATATTTAAGTTGTTGCAGTTAAAAAGCTCGTAG67480.10475698877991796No Hit
GTCGAATGGGGAGCCCACAGGCCGACGCCCGGAGCACGCTGATGCCGAGG67280.10444650570706698No Hit
GTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAAGCTGATTGG66590.10337533910573111No Hit
CTTACGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGTAAGCG65790.10213340681432723No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA65010.10092252283020846No Hit
CTGCAGACCACGATCACGGCAGCGACGTCTCCACAAGCGTATCAAAAGCC64720.10047232237457454No Hit
CCGAAGGCCAACACAATAGGATCGAAATCCTATGATGTTATCCCATGCTA64620.10031708083814905No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)