FastQCFastQC Report
Tue 8 Nov 2022
H3TCYBGXN_n01_EV-phd-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3TCYBGXN_n01_EV-phd-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4367228
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACCGATATGCTTCGGGGAGCTGTAAGTGAGCTTTGATCCGGAGATTTCC279350.6396505975873025No Hit
CTAACACCGATATGCTTCGGGGAGCTGTAAGTGAGCTTTGATCCGGAGAT198850.45532314777245425No Hit
CTTCAATATCCTATGTATTCAGATAAAGATACTGTTCCATTACGAACAGT193680.44348497490856903No Hit
GCTTTCTCTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCGGGTCTGCCT166100.3803327877546123No Hit
CTTTCTCTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCGGGTCTGCCTT162650.3724330399054045No Hit
CTGCCTTCAATATCCTATGTATTCAGATAAAGATACTGTTCCATTACGAA131780.30174747001988445No Hit
CGGGACTAACACCGATATGCTTCGGGGAGCTGTAAGTGAGCTTTGATCCG131310.300671272486804No Hit
CGCACGGTGAATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACTAACA130160.29803802320373474No Hit
CTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGCGATACTTGGTGTG124970.2861540547001439No Hit
CGGATATCTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGCGATACT101810.23312270392111425No Hit
CCTCGATCGATTAGTATCAGTCAGCTACACATGTCGCCACGCTTCCACCT98890.2264365405241036No Hit
GGGACTAACACCGATATGCTTCGGGGAGCTGTAAGTGAGCTTTGATCCGG95350.21833071229622084No Hit
CCGATATGCTTCGGGGAGCTGTAAGTGAGCTTTGATCCGGAGATTTCCGA90900.20814118246173544No Hit
GGCAGACGTGCCCTCGGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAG88970.20372190323014963No Hit
CCCAGGCAGACGTGCCCTCGGCCAGAAGGCTTGGGGCGCAACTTGCGTTC84570.19364686249492813No Hit
GGTGAATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACTAACACCGAT77000.17631321286637655No Hit
CTCTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCGGGTCTGCCTTCAAT71600.16394839014587742No Hit
CTTGGTTTGGGCTAATTCCGTTTCGCTCGCCGCTACTCAGGAAATCGCAT66610.15252237803934213No Hit
CTTTGATCCGGAGATTTCCGAATGGGGAAACCCACTGTTCGTAATGGAAC65080.14901901160186737No Hit
CTTAGATGTTTCAGTTCCCCGGGTCTGCCTTCAATATCCTATGTATTCAG64950.14872133994378128No Hit
CGGTGAATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACTAACACCGA61700.14127954849162902No Hit
CGGGTGAGTAACACGTGGGCAACCTGCCTGTAAGACTGGGATAACTTCGG56740.12992222984465204No Hit
CTCAGTCCCAGTGTGGCCGATCACCCTCTCAGGTCGGCTATGCATCGTTG54490.12477022037777741No Hit
CGCGAATCCTCTTGAAAGAGAGGGGTGCCTTCGGGAACGCGGACACAGGT51050.11689337034842238No Hit
GACGGGACTAACACCGATATGCTTCGGGGAGCTGTAAGTGAGCTTTGATC50950.11666439214989462No Hit
CCGATAGCGAACAAGTACCGCGAGGTAAAGATGAAAAGGACTTTGAAAAG50660.11600035537416413No Hit
CTACAACCCCAAGAGGCAAGCCTCTTGGTTTGGGCTAATTCCGTTTCGCT50110.11474097528226142No Hit
TTTCTCTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCGGGTCTGCCTTC50000.11448909926388087No Hit
CGAATTCAGTCAAACTCCGAATGCCAAAGACTTATCCATGGGAGTCAGAC48880.11192454344036995No Hit
CTTACGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGTAAGCG48490.1110315284661117No Hit
CAGGAATATCAACCTGTTGGCCATCGCCTACGCTTTTCAGCCTCGGCTTA48350.1107109589881728No Hit
CTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTC48300.11059646988890895No Hit
TGAAAATAAGCGTAGATCCGGAGATTCCCGAATAGGTTAACCTTTTGAAC47740.10931419197715347No Hit
CGGCAACGGATATCTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGC47390.1085127682823063No Hit
CTGCAAAGGATTCTACCCGCCGCTCGGTGGTAATTATAATTCAAGGCGGT46930.10745946856907859No Hit
GTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGATCTTAGTTGCCAGCA46920.10743657074922583No Hit
CGATATGCTTCGGGGAGCTGTAAGTGAGCTTTGATCCGGAGATTTCCGAA46770.10709310345143419No Hit
CTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCGGGTCTGCCTTCAATAT46410.10626878193673422No Hit
CTTTTATCCGTTGAGCGATGGCCCTTCCATGCGGAACCACCGGATCACTA45310.10375002175292887No Hit
CTTGGCACTAGGAGCCGATGAAGGACGGGACTAACACCGATATGCTTCGG44880.10276541549925948No Hit
AAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGCGAACCGGGAAGAG44720.10239905038161506No Hit
CGAATGGGGAGCCCACAGGCCGACGCCCGGAGCACGCTGATGCCGAGGCA44640.10221586782279285No Hit
GTTTGGGCTAATTCCGTTTCGCTCGCCGCTACTCAGGAAATCGCATTTGC44520.10194109398455953No Hit
GGAAATTTGAGAGGAGCTGTCCTTAGTACGAGAGGACCGGGATGGACACA43780.10024665531545411No Hit
CTGGCAACGTCCTACTCTCACAGGGACAAAGTCCCAACTACCATTGGCGC43700.10006347275663188No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)