FastQCFastQC Report
Wed 25 Jan 2023
H3TCYAFX5_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3TCYAFX5_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences188816695
Sequences flagged as poor quality0
Sequence length91
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA53085122.8114632554075794Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4947480.2620255587039059No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAAAAAAAAAAAAAAAAAAAA4476390.23707596407192702Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA3833240.20301382777619317Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAA3830110.20284805853634924Clontech SMARTer II A Oligonucleotide (100% over 24bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTAAAAAAAAAAAAAAAAAAA2851860.15103855090779975Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)